LOCUS       V024217                10633 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024217
VERSION     V024217
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..5330
                     /label="TRIM28(NM_005762)"
                     /note="TRIM28(NM_005762)"
                     /gene="TRIM28"
     CDS             5331..5360
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     promoter        5421..5920
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5941..6540
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6554..7142
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7349..7982
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8111..8127)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8135..8151
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8159..8189)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8204..8225
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8513..9101)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9272..10132)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10133..10237)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10285..10419
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatggc ggcctccgcg gcggcagcct cggcagcagc ggcctcggcc gcctctggca
     2881 gcccgggccc gggcgagggc tccgctggcg gcgaaaagcg ctccaccgcc ccttcggccg
     2941 cagcctcggc ctctgcctca gccgcggcgt cgtcgcccgc ggggggcggc gccgaggcgc
     3001 tggagctgct ggagcactgc ggcgtgtgca gagagcgcct gcgacccgag agggagcccc
     3061 gcctgctgcc ctgtttgcac tcggcctgta gtgcctgctt agggcccgcg gcccccgccg
     3121 ccgccaacag ctcgggggac ggcggggcgg cgggcgacgg caccgtggtg gactgtcccg
     3181 tgtgcaagca acagtgcttc tccaaagaca tcgtggagaa ttatttcatg cgtgatagtg
     3241 gcagcaaggc tgccaccgac gcccaggatg cgaaccagtg ctgcactagc tgtgaggata
     3301 atgccccagc caccagctac tgtgtggagt gctcggagcc tctgtgtgag acctgtgtag
     3361 aggcgcacca gcgggtgaag tacaccaagg accatactgt gcgctctact gggccagcca
     3421 agtctcggga tggtgaacgt actgtctatt gcaacgtaca caagcatgaa ccccttgtgc
     3481 tgttttgtga gagctgtgat actctcacct gccgagactg ccagctcaat gcccacaagg
     3541 accaccagta ccagttctta gaggatgcag tgaggaacca gcgcaagctc ctggcctcac
     3601 tggtgaagcg ccttggggac aaacatgcaa cattgcagaa gagcaccaag gaggttcgca
     3661 gctcaatccg ccaggtgtct gacgtacaga agcgtgtgca agtggatgtc aagatggcca
     3721 tcctgcagat catgaaggag ctgaataagc ggggccgtgt gctggtcaat gatgcccaga
     3781 aggtgactga ggggcagcag gagcgcctgg agcggcagca ctggaccatg accaagatcc
     3841 agaagcacca ggagcacatt ctgcgctttg cctcttgggc tctggagagt gacaacaaca
     3901 cagccctttt gctttctaag aagttgatct acttccagct gcaccgggcc ctcaagatga
     3961 ttgtggatcc cgtggagcca catggcgaga tgaagtttca gtgggacctc aatgcctgga
     4021 ccaagagtgc cgaggccttt ggcaagattg tggcagagcg tcctggcact aactcaacag
     4081 gccctgcacc catggcccct ccaagagccc cagggcccct gagcaagcag ggctctggca
     4141 gcagccagcc catggaggtg caggaaggct atggctttgg gtcaggagat gatccctact
     4201 caagtgcaga gccccatgtg tcaggtgtga aacggtcccg ctcaggtgag ggcgaggtga
     4261 gcggccttat gcgcaaggtg ccacgagtga gccttgaacg cctggacctg gacctcacag
     4321 ctgacagcca gccacccgtc ttcaaggtct tcccaggcag taccactgag gactacaacc
     4381 ttattgttat tgaacgtggc gctgccgctg cagctaccgg ccagccaggg actgcgcctg
     4441 caggaacccc tggtgcccca cccctggctg gcatggccat tgtcaaggag gaggagacgg
     4501 aggctgccat tggagcccct cctactgcca ctgagggccc tgagaccaaa cctgtgctta
     4561 tggctcttgc ggagggtcct ggtgctgagg gtccccgcct ggcctcacct agtggcagca
     4621 ccagctcagg gctggaggtg gtggctcctg agggtacctc agccccaggt ggtggcccgg
     4681 gaaccctgga tgacagtgcc accatttgcc gtgtctgcca gaagccaggc gatctggtta
     4741 tgtgcaacca gtgtgagttt tgtttccacc tggactgtca cctgccggcc ctgcaggatg
     4801 taccagggga ggagtggagc tgctcactct gccatgtgct ccctgacctg aaggaggagg
     4861 atggcagcct cagcctggat ggtgcagaca gcactggcgt ggtggccaag ctctcaccag
     4921 ccaaccagcg gaaatgtgag cgtgtactgc tggccctatt ctgtcacgaa ccctgccgcc
     4981 ccctgcatca gctggctacc gactccacct tctccctgga ccagcccggt ggcaccctgg
     5041 atctgaccct gatccgtgcc cgcctccagg agaagttgtc acctccctac agctccccac
     5101 aggagtttgc ccaggatgtg ggccgcatgt tcaagcaatt caacaagtta actgaggaca
     5161 aggcagacgt gcagtccatc atcggcctgc agcgcttctt cgagacgcgc atgaacgagg
     5221 ccttcggtga caccaagttc tctgctgtgc tggtggagcc cccgccgatg agcctgcctg
     5281 gtgctggcct gagttcccag gagctgtctg gtggccctgg tgatggcccc gagcagaagc
     5341 tgatctcaga ggaggacctg tgaattctgc agtcgacggt accgcgggcc cgggatcccg
     5401 cgactctaga taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     5461 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     5521 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     5581 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     5641 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     5701 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     5761 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     5821 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     5881 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     5941 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6001 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6061 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6121 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6181 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6241 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6301 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6361 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6421 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6481 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6541 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6601 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6661 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6721 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6781 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6841 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6901 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     6961 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7021 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7081 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7141 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     7201 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     7261 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7321 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     7381 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     7441 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     7501 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     7561 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     7621 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     7681 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     7741 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     7801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7981 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     8041 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg
     8101 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8161 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8221 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8281 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8341 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8401 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8701 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8941 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9001 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9061 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9121 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9181 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9241 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10261 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
    10321 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10381 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    10441 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    10501 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    10561 taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta
    10621 caaacttagt agt
//
