LOCUS       V030265                13531 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030265
VERSION     V030265
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..8227
                     /label="TET3(NM_001287491)"
                     /note="TET3(NM_001287491)"
                     /gene="TET3"
     CDS             8234..8299
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8319..8818
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             8839..9438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9452..10040
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10247..10880
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(11009..11025)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11033..11049
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11057..11087)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11102..11123
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11411..11999)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12170..13030)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13031..13135)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    13183..13317
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgagcca gtttcaggtg cccctggccg tccagccgga
     2881 cctgccaggc ctttatgact tccctcagcg ccaggtgatg gtagggagct tcccggggtc
     2941 tgggctctcc atggctggga gtgagtccca actccgaggg ggtggagatg gtcgaaagaa
     3001 acggaaacgg tgtggtactt gtgagccctg ccggcggctg gaaaactgtg gcgcttgcac
     3061 tagctgtacc aaccgccgca cgcaccagat ctgcaaactg cgaaaatgtg aggtgctgaa
     3121 gaaaaaagta gggcttctca aggaggtgga aataaaggct ggtgaaggag ccgggccgtg
     3181 gggacaagga gcggctgtca agacaggctc agagctcagc ccagttgatg gacctgttcc
     3241 aggtcagatg gactcagggc cagtgtacca tggggactca cggcagctaa gcgcctcagg
     3301 ggtgccggtc aatggtgcta gagagcccgc tggacccagt ctgctgggga ctgggggtcc
     3361 ttggcgggta gaccaaaagc ccgactggga ggctgcccca ggcccagctc atactgctcg
     3421 cctggaagat gcccacgatc tggtggcctt ttcggctgtg gccgaagctg tgtcctctta
     3481 tggggccctt agcacccggc tctatgaaac cttcaaccgt gagatgagtc gtgaggctgg
     3541 gaacaacagc aggggacccc ggccagggcc tgagggctgc tctgctggca gcgaagacct
     3601 tgacacactg cagacggccc tggccctcgc gcggcatggt atgaaaccac ccaactgcaa
     3661 ctgcgatggc ccagaatgcc ctgactacct cgagtggctg gaggggaaga tcaagtctgt
     3721 ggtcatggaa ggaggggagg agcggcccag gctcccaggg cctctgcctc ctggtgaggc
     3781 cggcctccca gcaccaagca ccaggccact cctcagctca gaggtgcccc agatctctcc
     3841 ccaagagggc ctgcccctgt cccagagtgc cctgagcatt gccaaggaaa aaaacatcag
     3901 cttgcagacc gccattgcca ttgaggccct cacacagctc tcctctgccc tcccgcagcc
     3961 ttctcattcc accccccagg cttcttgccc ccttcctgag gccttgtcac ctcctgcccc
     4021 tttcagatct ccccagtctt acctccgggc tccctcatgg cctgtggttc ctcctgaaga
     4081 gcactcatct tttgctcctg atagctctgc cttccctcca gcaactccta gaactgagtt
     4141 ccctgaagcc tggggcactg acacccctcc agcaacgccc cggagctcct ggcccatgcc
     4201 tcgcccaagc cccgatccca tggctgaact ggagcagttg ttgggcagcg ccagtgatta
     4261 catccagtca gtattcaagc ggcctgaggc cctgcctacc aagcccaagg tcaaggtgga
     4321 ggcaccctct tcctccccgg ccccggcccc atcccctgta cttcagaggg aggctcccac
     4381 gccatcctcg gagcccgaca cccaccagaa ggcccagacc gccctgcagc agcacctcca
     4441 ccacaagcgc agcctcttcc tagaacaggt gcacgacacc tccttccctg ctccttcaga
     4501 gccttctgct cctggctggt ggcccccacc aagttcacct gtcccacggc ttccagacag
     4561 accacccaag gagaagaaga agaagctccc aacaccagct ggaggtcccg tgggaacgga
     4621 gaaagctgcc cctgggatca agcccagtgt ccgaaagccc attcagatca agaagtccag
     4681 gccccgggaa gcacagcccc tcttcccacc tgtccgacag attgtcctgg aagggcttag
     4741 gtccccagcc tcccaggaag tgcaggctca tccaccggcc cctctgcctg cctcacaggg
     4801 ctctgctgtg cccctgcccc cagaaccttc tcttgcgcta tttgcaccta gtccctccag
     4861 ggacagcctg ctgcccccta ctcaggaaat gaggtccccc agccccatga cagccttgca
     4921 gccaggctcc actggccctc ttccccctgc cgatgacaag ctggaagagc tcatccggca
     4981 gtttgaggct gaatttggag atagctttgg gcttcccggc cccccttctg tgcccattca
     5041 ggaccccgag aaccagcaaa catgtctccc agcccctgag agcccctttg ctacccgttc
     5101 ccccaagcaa atcaagattg agtcttcggg ggctgtgact gtgctctcaa ccacctgctt
     5161 ccattcagag gagggaggac aggaggccac acccaccaag gctgagaacc cactcacacc
     5221 caccctcagt ggcttcttgg agtcacctct taagtacctg gacacaccca ccaagagtct
     5281 gctggacaca cctgccaaga gagcccaggc cgagttcccc acctgcgatt gcgtcgaaca
     5341 aatagtggag aaagatgaag gtccatatta tactcacttg ggatctggcc ccacggtcgc
     5401 ctctatccgg gaactcatgg aggagcggta tggagagaag gggaaagcca tccggatcga
     5461 gaaggtcatc tacacgggga aggagggaaa gagctcccgc ggttgcccca ttgcaaagtg
     5521 ggtgatccgc aggcacacgc tggaggagaa gctactctgc ctggtgcggc accgggcagg
     5581 ccaccactgc cagaacgctg tgatcgtcat cctcatcctg gcctgggagg gcattccccg
     5641 tagcctcgga gacaccctct accaggagct caccgacacc ctccggaagt atgggaaccc
     5701 caccagccgg agatgcggcc tcaacgatga ccggacctgc gcttgccaag gcaaagaccc
     5761 caacacctgt ggtgcctcct tctcctttgg ttgttcctgg agcatgtact tcaacggctg
     5821 caagtatgct cggagcaaga cacctcgcaa gttccgcctc gcaggggaca atcccaaaga
     5881 ggaagaagtg ctccggaaga gtttccagga cctggccacc gaagtcgctc ccctgtacaa
     5941 gcgactggcc cctcaggcct atcagaacca ggtgaccaac gaggaaatag cgattgactg
     6001 ccgtctgggg ctgaaggaag gacggccctt cgcgggggtc acggcctgca tggacttctg
     6061 tgcccacgcc cacaaggacc agcataacct ctacaatggg tgcaccgtgg tctgcaccct
     6121 gaccaaggaa gacaatcgct gcgtgggcaa gattcccgag gatgagcagc tgcatgttct
     6181 ccccctgtac aagatggcca acacggatga gtttggtagc gaggagaacc agaatgcaaa
     6241 ggtgggcagc ggagccatcc aggtgctcac cgccttcccc cgcgaggtcc gacgcctgcc
     6301 cgagcctgcc aagtcctgcc gccagcggca gctggaagcc agaaaggcag cagccgagaa
     6361 gaagaagatt cagaaggaga agctgagcac tccggagaag atcaagcagg aggccctgga
     6421 gctggcgggc attacgtcgg acccaggcct gtctctgaag ggtggattgt cccagcaagg
     6481 cctgaagccc tccctcaagg tggagccgca gaaccacttc agctccttca agtacagcgg
     6541 caacgcggtg gtggagagct actcggtgct gggcaactgc cggccctccg acccttacag
     6601 catgaacagc gtgtactcct accactccta ctatgcacag cccagcctga cctccgtcaa
     6661 tggcttccac tccaagtacg ctctcccgtc ttttagctac tatggctttc catccagcaa
     6721 ccccgtcttc ccctctcagt tcctgggtcc tggtgcctgg gggcacagtg gcagcagtgg
     6781 cagttttgag aagaagccag acctccacgc tctgcacaac agcctgagcc cggcctacgg
     6841 tggtgctgag tttgccgagc tgcccagcca ggctgttccc acagacgccc accaccccac
     6901 tcctcaccac cagcagcctg cgtacccagg ccccaaggag tatctgcttc ccaaggcccc
     6961 cctactccac tcagtgtcca gggacccctc cccctttgcc cagagctcca actgctacaa
     7021 cagatccatc aagcaagagc cagtagaccc gctgacccag gctgagcctg tgcccagaga
     7081 cgctggcaag atgggcaaga cacctctgtc cgaggtgtct cagaatggag gacccagtca
     7141 cctttgggga cagtactcag gaggcccaag catgtccccc aagaggacta acggtgtggg
     7201 tggcagctgg ggtgtgttct cgtctgggga gagtcctgcc atcgtccctg acaagctcag
     7261 ttcctttggg gccagctgcc tggccccttc ccacttcaca gatggccagt gggggctgtt
     7321 ccccggtgag gggcagcagg cagcttccca ctctggagga cggctgcgag gcaaaccgtg
     7381 gagcccctgc aagtttggga acagcacctc ggccttggct gggcccagcc tgactgagaa
     7441 gccgtgggcg ctgggggcag gggatttcaa ctcggccctg aaaggtagtc ctgggttcca
     7501 agacaagctg tggaacccca tgaaaggaga ggagggcagg attccagccg caggggccag
     7561 ccagctggac agggcctggc agtcctttgg tctgcccctg ggatccagcg agaagctgtt
     7621 tggggctctg aagtcagagg agaagctgtg ggaccccttc agcctggagg aggggccggc
     7681 tgaggagccc cccagcaagg gagcggtgaa ggaggagaag ggcggtggtg gtgcggagga
     7741 ggaagaggag gagctgtggt cggacagtga acacaacttc ctggacgaga acatcggcgg
     7801 cgtggccgtg gccccagccc acggctccat cctcatcgag tgtgcccggc gggagctgca
     7861 cgccaccacg ccgcttaaga agcccaaccg ctgccacccc acccgcatct cgctggtctt
     7921 ctaccagcac aagaacctca accagcccaa ccacgggctg gccctctggg aagccaagat
     7981 gaagcagctg gcggagaggg cacgggcacg gcaggaggag gctgcccggc tgggcctggg
     8041 ccagcaggag gccaagctct acgggaagaa gcgcaagtgg gggggcactg tggttgctga
     8101 gccccagcag aaagagaaga agggggtcgt ccccacccgg caggcactgg ctgtgcccac
     8161 agactcggcg gtcaccgtgt cctcctatgc ctacacgaag gtcactggcc cctacagccg
     8221 ctggatcgga tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     8281 caaggatgac gatgacaagt agtctagata attctaccgg gtaggggagg cgcttttccc
     8341 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     8401 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     8461 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     8521 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     8581 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     8641 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     8701 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     8761 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     8821 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     8881 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     8941 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     9001 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     9061 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     9121 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     9181 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     9241 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     9301 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     9361 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     9421 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     9481 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     9541 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     9601 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     9661 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     9721 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     9781 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     9841 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     9901 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     9961 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
    10021 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
    10081 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
    10141 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
    10201 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
    10261 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    10321 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    10381 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    10441 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    10501 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    10561 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    10621 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    10681 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10741 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10801 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10861 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
    10921 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
    10981 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    11041 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    11101 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    11161 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    11221 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    11281 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    11341 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    11401 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    11461 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    11521 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    11581 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    11641 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    11701 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    11761 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    11821 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    11881 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    11941 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    12001 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    12061 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    12121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    12181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    12241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    12301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    12361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    12421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    12481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    12541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    12601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    12661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    12721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    12781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    12841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    12901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    12961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    13021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    13081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    13141 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
    13201 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    13261 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    13321 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    13381 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    13441 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta
    13501 tttgttaaat atgtactaca aacttagtag t
//
