LOCUS       V040496                10303 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040496
VERSION     V040496
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4978
                     /label="TAB3(NM_152787)"
                     /note="TAB3(NM_152787)"
                     /gene="TAB3"
     CDS             4985..5011
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5015..5041
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5045..5071
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5091..5590
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5611..6210
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6224..6812
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7019..7652
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7781..7797)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7805..7821
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7829..7859)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7874..7895
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8183..8771)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8942..9802)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9803..9907)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9955..10089
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggcgca aagcagccca cagcttgata ttcaggttct
     2881 ccatgatctt cgacaacgtt tccctgaaat tccagagggc gtggtgtctc agtgcatgtt
     2941 acagaataac aacaatcttg aagcctgttg ccgagccctt tcccaggaga gtagcaaata
     3001 cttatatatg gaataccata gtccagatga caataggatg aatagaaatc gccttttaca
     3061 tattaacctg ggtatccatt ctcctagtag ctatcaccca ggagatggag cccaacttaa
     3121 tggtggtcga acactggtac atagctcaag tgatggacat attgatcctc agcatgcagc
     3181 aggtaaacag ctgatatgtt tagttcaaga accacactca gctccagctg ttgttgctgc
     3241 tactcccaac tacaatccat tttttatgaa cgaacagaac agaagtgcag ctactcctcc
     3301 ttcacaacca cctcaacagc catcttccat gcaaacagga atgaatccgt ctgctatgca
     3361 agggccttca ccaccaccgc cacctccttc atacatgcac atacctcggt atagtacaaa
     3421 tccaattact gttacagtat cccagaacct cccttctgga cagactgtac caagagcttt
     3481 acaaattctt ccacaaattc caagcaatct ctatgggtct cctggttcta tttatattag
     3541 acagacatct cagagttcat caggaagaca aactcctcag agtacgccgt ggcagtcctc
     3601 accacagggc ccagtgcctc actatagcca gcgtccttta cctgtttatc cacaccaaca
     3661 gaactatcag ccttctcagt attctcccaa acagcagcag atccctcagt ctgcttacca
     3721 ttcaccacct ccttctcaat gtccttcacc cttcagctct ccacagcatc aagtgcaacc
     3781 ttcccagttg ggccacatct ttatgccacc tagtccttca actactccac cccatccata
     3841 tcaacaagga cctcctagct atcagaaaca gggaagccat tcagtagcct atcttccata
     3901 cacagcatct agtttatcca aaggttccat gaagaagata gaaattacag ttgaaccttc
     3961 tcaaagacct gggacagcaa ttaataggag tccttcaccc atcagtaatc aaccatctcc
     4021 acggaatcaa cactcactgt acacagccac cacgccacct tcaagttctc cttcaagagg
     4081 gatatctagt caaccaaaac ctccatttag tgttaatcct gtgtatatta catatacaca
     4141 gccaactgga ccttcttgta ctccatcacc atctcctcga gtgataccaa acccaactac
     4201 agtttttaaa attaccgtag gccgagcaac gactgaaaat cttttaaatt tagtggacca
     4261 agaagagcgc tctgcagcac cagaacctat tcagcccatt tcagtgatac caggctctgg
     4321 gggagaaaag ggaagccata aatatcagag aagttctagt tctggatcag atgactatgc
     4381 ctacacacaa gccttgctgt tacatcaacg agcaaggatg gagaggttag caaagcaact
     4441 gaaacttgag aaagaggagc tagagcggtt gaagtctgaa gttaatggta tggagcatga
     4501 cctgatgcag agacggctca gaagagtcag ctgcaccact gcgatcccta cgcctgagga
     4561 aatgacaaga ttgagaagca tgaacagaca actccagata aatgttgact gtacactgaa
     4621 agaagttgac ctccttcaat ctagaggaaa ctttgatcca aaagccatga ataattttta
     4681 tgacaacata gaacctggcc cagttgtacc acccaagcca tctaaaaaag actcctcaga
     4741 cccctgcaca attgagagaa aagcccgaag aattagcgtg acctccaaag tacaggcaga
     4801 catccatgac acccaggcag cagctgcaga tgagcatcga actggctcca cacaaagtcc
     4861 tcggacacaa cctcgagatg aagactacga aggggctcca tggaattgtg atagctgcac
     4921 ctttcttaac cacccagcac taaatcgctg tgagcagtgc gagatgccac ggtacaccgg
     4981 atcctaccca tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc
     5041 tagctatcct tatgatgtcc cagactacgc atagtctaga taattctacc gggtagggga
     5101 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     5161 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     5221 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     5281 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     5341 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     5401 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     5461 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     5521 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     5581 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     5641 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5701 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     5761 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     5821 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     5881 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     5941 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6001 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6061 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6121 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6181 tggtgcatga cccgcaagcc cggtgcctga ccgcgtctgg aacaatcaac ctctggatta
     6241 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6301 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6361 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6421 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6481 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6541 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6601 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     6661 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6721 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6781 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta
     6841 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6901 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6961 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg
     7021 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     7081 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     7141 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     7201 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     7261 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     7321 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     7381 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     7441 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     7501 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7561 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7621 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7681 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg
     7741 ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7801 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7861 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7921 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7981 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8041 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8101 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8161 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8221 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8281 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8341 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8401 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8461 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8521 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8581 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8641 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8701 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8761 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8821 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8881 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8941 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9001 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9061 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9121 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9181 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9241 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9301 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9361 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9421 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9481 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9541 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9601 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9661 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9721 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9781 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9841 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9901 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg
     9961 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    10021 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10081 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    10141 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    10201 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt
    10261 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
