LOCUS       V045067                10339 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045067
VERSION     V045067
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2840..2860
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             4634..4660
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4661..5095
                     /label="SYNJ2BP(NM_018373)"
                     /note="SYNJ2BP(NM_018373)"
                     /gene="SYNJ2BP"
     promoter        5127..5626
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5647..6246
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6260..6848
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7055..7688
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7817..7833)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7841..7857
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7865..7895)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7910..7931
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8219..8807)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8978..9838)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9839..9943)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9991..10125
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcatgc ccaagaagaa gaggaaagtc tccaacctgc tgactgtgca
     2881 ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat
     2941 ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt
     3001 gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc
     3061 tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat
     3121 ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc
     3181 tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg
     3241 ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct
     3301 gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta
     3361 caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac
     3421 cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg
     3481 tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc
     3541 tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc
     3601 tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac
     3661 ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg
     3721 ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga
     3781 aatcatgcag gctggtggct ggaccaatgt gaacattgtg atgaactaca tcagaaacct
     3841 ggactctgag actggggcca tggtgaggct gctcgaggat ggggacgtta acggaagcgg
     3901 aggtggaggc ggatcaggcg agagcctttt caagggcccg agggactaca acccgatctc
     3961 cagcaccatc tgtcacctga ccaacgagag cgacggtcac accactagtc tgtacggcat
     4021 cggcttcggc cccttcatca tcaccaacaa gcatctgttc aggaggaata acggcacact
     4081 gctggtgcaa agcctgcacg gcgtgttcaa agtgaagaac acaaccaccc tgcaacagca
     4141 cctgatcgac ggcagggaca tgattatcat caggatgccc aaggacttcc ccccctttcc
     4201 ccagaaactg aagttcaggg agccacaaag ggaggagcga atctgcctgg tgaccaccaa
     4261 cttccagacc aagtccatga gcagcatggt ctctgatacc agctgcacct tccccagcag
     4321 cgacggcatc ttctggaagc actggattca gacgaaggat ggccaatgcg gcagcccatt
     4381 ggtgagcact agggacggct tcatcgtggg catccacagc gccagcaatt ttaccaatac
     4441 caacaactac ttcacgagcg tgccgaaaaa cttcatggag ctgttgacca atcaagaggc
     4501 gcagcagtgg gtgagcggct ggaggctgaa cgccgacagc gttctttggg gcggacataa
     4561 ggtgttcatg gtcaagcccg aggaaccctt ccagcccgtt aaggaagcca ctcagcttgg
     4621 aggcggtgga tcttacccat acgatgttcc agattacgct atgaacggaa gagtggatta
     4681 tttggtcact gaggaagaga tcaatcttac cagagggccc tcagggctgg gcttcaacat
     4741 cgtcggtggg acagatcagc agtatgtctc caacgacagt ggcatctacg tcagccgcat
     4801 caaagaaaat ggggctgcgg ccctggatgg gcggctccag gagggtgata agatcctttc
     4861 ggtaaatggc caagacctaa agaacctgct gcaccaggat gctgtagacc tctttcgtaa
     4921 tgcaggctat gctgtgtctc tgagagtgca gcacaggtta caggtgcaga atggacctat
     4981 aggacatcga ggtgaagggg acccaagtgg tattcccata tttatggtgc tggtgccagt
     5041 gtttgccctc accatggtag cagcctgggc tttcatgaga taccggcaac aactttgagg
     5101 atcccgcgac tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     5161 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     5221 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     5281 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     5341 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     5401 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     5461 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     5521 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     5581 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     5641 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5701 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5761 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5821 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5881 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5941 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6001 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6061 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6121 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6181 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6241 gcctgaccgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6301 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6361 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6421 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6481 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6541 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6601 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6661 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6721 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6781 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6841 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     6901 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     6961 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7021 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     7081 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     7141 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     7201 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     7261 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     7321 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     7381 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     7441 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     7501 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7561 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7621 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7681 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7741 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga
     7801 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7861 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7921 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7981 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8041 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8101 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8161 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8221 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8281 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8341 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8401 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8461 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8521 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8581 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8641 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8701 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8761 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8821 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8881 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9961 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    10021 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10081 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10141 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10201 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    10261 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat
    10321 gtactacaaa cttagtagt
//
