LOCUS       V028655                12088 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028655
VERSION     V028655
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..6784
                     /label="RNF123(NM_022064)"
                     /note="RNF123(NM_022064)"
                     /gene="RNF123"
     CDS             6791..6856
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6876..7375
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7396..7995
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8009..8597
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8804..9437
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9566..9582)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9590..9606
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9614..9644)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9659..9680
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9968..10556)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10727..11587)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11588..11692)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11740..11874
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggcatc caagggggcc ggcatgtctt tctcccgcaa
     2881 gagctatagg ctgacctcag atgctgagaa atccagggtc acaggcattg tgcaggagaa
     2941 gctgctgaat gactacctga accgcatctt ttcctcttct gaacatgcac ccccagcagc
     3001 caccagcagg aaacccctga acttccagaa cctgccagaa catttggacc agttgctaca
     3061 ggtggacaat gaggaggagg aaagccaggg acaggttgaa gggcggcttg gcccatccac
     3121 tgtggtcctg gaccacacag gcggctttga ggggcttctc ctggtggatg atgacctgct
     3181 gggggtgatt ggacacagca actttggcac catccgctct accacatgcg tgtacaaagg
     3241 gaaatggctc tacgaggtcc tcatctcctc ccaggggctc atgcagatcg gctggtgcac
     3301 catcagctgc cgcttcaacc aggaggaggg ggttggagat acacacaact cctatgccta
     3361 tgatggcaac cgcgtgcgca agtggaatgt gaccacaacg aattatggca aggcgtgggc
     3421 agcgggggac atcgtgagct gcctgattga cctggatgat ggcactctgt ccttctgcct
     3481 gaacggtgta tcactgggca ctgcctttga gaacctgtcc aggggcctgg gtatggccta
     3541 cttcccagcc atcagcctct ctttcaagga gtccgtggcc ttcaactttg gcagccgtcc
     3601 tctgcgctac ccagtggcag gctaccggcc cctgcaggac ccaccgagtg ctgacctggt
     3661 gcgggcacag aggttgctgg gctgcttccg ggcagtgctg agtgtggagc tggaccctgt
     3721 ggaggggcgg ctgttggaca aggagagctc caagtggcgg ttgcggggcc agcccaccgt
     3781 cctcctcaca ctggcccaca tcttccatca ctttgcaccg cttctgcgca aggtgtatct
     3841 ggtggaggct gtgctcatga gcttcttgct gggcatcgtg gagaagggca cacccacaca
     3901 ggcacagtcc gtggtgcacc aggtcctgga cctcttgtgg ctcttcatgg aggactacga
     3961 ggtacaagat tgcctcaagc agttgatgat gtctctgctt cggctgtacc gattctcacc
     4021 cattgtccca gacctgggcc tacagatcca ttacctgcgg ctcactatcg ccatcctgag
     4081 gcatgagaag tcccgcaagt ttctgcttag caatgtcctc ttcgacgtgc tccgctccgt
     4141 cgtcttcttt tacatcaaga gccccctgcg tgtggaggag gccggcctgc aggagctcat
     4201 tcccaccacc tggtggcccc actgctccag tagggagggc aaagagagca cggagatgaa
     4261 ggaggagacc gcagaggagc ggctgcggcg gcgagcctac gaacggggct gtcagcggct
     4321 caggaagcgc atcgaagtgg tggaagaact acaggtccag atcctgaagc tgctgctgga
     4381 caataaagat gacaatgggg gtgaagcttc taggtatatc ttcctgacca agtttcgcaa
     4441 gtttctgcag gagaacgcca gtggccgggg gaacatgccc atgctctgcc cccctgagta
     4501 catggtctgc ttcttacacc ggctgatctc tgccctgcgc tactattggg atgaatacaa
     4561 ggcttccaat cctcatgctt ccttcagtga ggaggcctac atcccgcccc aggtcttcta
     4621 taatggcaag gtggactact ttgacctgca gcgcctgggg ggcctcctct cgcacctgcg
     4681 gaagaccctc aaagatgacc ttgcttccaa agccaacatt gtgatcgacc cactggagct
     4741 ccagtcaacc gccatggatg acctagatga ggatgaggag ccagccccag ctatggccca
     4801 gcgccccatg caggccctgg ctgttggggg gccactgccc ctgccccggc ccggctggct
     4861 cagttctcca actttgggcc gagccaaccg cttcctcagc acagcggctg tgagcctcat
     4921 gaccccacgg cggcctctga gcacctcgga gaaagtgaag gtccgcacgc tgagcgtgga
     4981 gcagaggacc cgtgaggaca ttgaaggcag ccactggaat gagggcttgc tgctggggcg
     5041 gccccccgag gagcctgagc agcccctcac cgagaactcg ctgctggaag tcctggatgg
     5101 ggcggtcatg atgtacaacc tcagcgtaca ccagcagctg ggcaagatgg tgggtgtctc
     5161 cgatgatgtc aatgaatacg ctatggctct gagggacaca gaggacaagc tccgccggtg
     5221 ccccaagagg aggaaggaca tccttgcaga gttgaccaag agccagaagg ttttctcaga
     5281 aaagctggac cacctgagcc gccgtcttgc ctgggtccat gccactgtct actcccagga
     5341 gaagatgctg gacatctact ggctgctgcg cgtctgcctg cggaccattg agcacggtga
     5401 tcgcacaggg tctctctttg ccttcatgcc cgagttctac ctgagcgtgg ccatcaacag
     5461 ctacagtgct ctcaagaatt actttggtcc cgtgcacagc atggaggagc tcccaggcta
     5521 tgaagagacc ctgacccgcc tggctgccat tctcgccaaa cactttgccg acgcacgcat
     5581 tgtgggcact gacatccgag actcactgat gcaggccctg gccagctacg tgtgctaccc
     5641 acactccctg cgggctgtgg agcgaatccc cgaggagcag cgtatcgcca tggtgaggaa
     5701 cctcctggcg ccctatgagc agcggccctg ggcccagacc aactggatcc tggtgcggct
     5761 ctggaggggc tgtggcttcg ggtaccgcta tacacggctg ccacatctgc tgaaaaccaa
     5821 acttgaggac gccaatttgc ccagcctcca gaagccctgc ccttccaccc tgctgcagca
     5881 gcacatggcg gacctcctac agcagggtcc tgatgtggca cccagcttcc tcaacagcgt
     5941 cctcaatcag ctcaactggg ccttctctga attcattggc atgatccaag agatccagca
     6001 ggctgctgag cgcctggagc ggaactttgt ggacagccgg cagctcaagg tatgtgccac
     6061 ctgctttgac ctctcggtca gcctgctgcg tgtcttggag atgactatca cactggtgcc
     6121 tgagatattc cttgactgga cccggcctac ctctgagatg ctgctgcggc gtcttgcaca
     6181 gctgctaaac caggtgctga accgggtgac agctgagagg aacctgtttg atcgtgtggt
     6241 caccctacgg ctgcctggcc tagagagcgt ggaccactat cccattctgg tggcagtgac
     6301 gggcatcctg gtgcagctcc tggtgcgtgg cccagcctca gagagagagc aagccacatc
     6361 agtgctcctg gcagatccct gcttccagct acgctcaata tgctatctcc tgggacagcc
     6421 agagccccca gcacctggca ctgctctgcc agcccctgac cggaagcgct tctccctgca
     6481 gagctatgcg gattatatca gtgccgatga gctggcccaa gtggaacaga tgctggcgca
     6541 cctgacctct gcatctgccc aggcagcagc tgcctccctg cccaccagtg aggaggacct
     6601 ctgccccatc tgctatgccc accccatctc tgctgtgttc cagccctgtg gccacaagtc
     6661 ctgcaaagcc tgtatcaacc agcacctgat gaacaacaag gactgcttct tctgcaaaac
     6721 caccatcgtg tctgtagagg actgggagaa gggagccaat acgagtacta cctcctcagc
     6781 tgccggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     6841 ggatgacgat gacaagtagt ctagataatt ctaccgggta ggggaggcgc ttttcccaag
     6901 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     6961 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     7021 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     7081 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     7141 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     7201 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     7261 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     7321 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     7381 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     7441 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     7501 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     7561 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     7621 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     7681 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7741 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     7801 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7861 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7921 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7981 aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     8041 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8101 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8161 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8221 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8281 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8341 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8401 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8461 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8521 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8581 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     8641 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     8701 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     8761 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     8821 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     8881 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     8941 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     9001 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     9061 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     9121 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     9181 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     9241 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9301 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9361 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9421 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9481 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct
     9541 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9601 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9661 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9721 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9781 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9841 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9901 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9961 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10021 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10081 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10141 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10201 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10261 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10321 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10381 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10441 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10501 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10561 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10741 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10801 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10861 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10921 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10981 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11041 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11101 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11161 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11221 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11281 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11341 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11401 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11461 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11521 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11581 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11641 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11701 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
    11761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    11821 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    11881 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    11941 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    12001 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt
    12061 gttaaatatg tactacaaac ttagtagt
//
