LOCUS       V022152                13037 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022152
VERSION     V022152
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..7733
                     /label="PTPN23(NM_015466)"
                     /note="PTPN23(NM_015466)"
                     /gene="PTPN23"
     CDS             7740..7805
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7825..8324
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             8345..8944
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8958..9546
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9753..10386
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(10515..10531)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10539..10555
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10563..10593)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10608..10629
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10917..11505)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11676..12536)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12537..12641)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12689..12823
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgga ggccgtgccc cgcatgccca tgatctggct ggacctgaag gaggccggtg
     2881 actttcactt ccagccagct gtgaagaagt ttgtcctgaa gaattatgga gagaacccag
     2941 aagcctacaa tgaagaactg aagaagctgg agttgctcag acagaatgct gtccgtgtcc
     3001 cacgagactt tgagggctgt agtgtcctcc gcaagtacct cggccagctt cattacctgc
     3061 agagtcgggt ccccatgggc tcgggccagg aggccgctgt ccctgtcacc tggacagaga
     3121 tcttctcagg caagtctgtg gcccatgagg acatcaagta cgagcaggcc tgtattctct
     3181 acaaccttgg agcgctgcac tccatgctgg gggccatgga caagcgggtg tctgaggagg
     3241 gcatgaaggt ctcctgtacc catttccagt gcgcagccgg cgccttcgcc tacctacggg
     3301 agcacttccc tcaagcctac agcgtcgaca tgagccgcca gatccttacg ctcaacgtca
     3361 acctcatgct gggccaggct caggagtgcc tcctggagaa gtcgatgttg gacaacagga
     3421 agagctttct ggtggcccgc atcagtgcac aggtggtaga ttactacaag gaggcatgcc
     3481 gggccttgga gaaccccgac actgcctcac tgctgggccg gatccagaag gactggaaga
     3541 aacttgtgca gatgaagatc tactacttcg cagccgtggc tcatctgcac atgggaaagc
     3601 aggccgagga gcagcagaag ttcggggagc gggttgcata cttccagagc gccctggaca
     3661 agctcaatga agccatcaag ttggccaagg gccagcctga cactgtgcaa gacgcgcttc
     3721 gcttcactat ggatgtcatt gggggaaagt acaattctgc caagaaggac aacgacttca
     3781 tttaccatga ggctgtccca gcattggaca ctcttcagcc tgtaaaagga gcccccttgg
     3841 tgaaaccctt gccagtgaac cccacagacc cagctgttac aggccctgac atctttgcca
     3901 aactggtacc catggctgcc cacgaggcct cgtcactgta cagtgaggag aaggccaagc
     3961 tgctccggga gatgatggcc aagattgagg acaagaatga ggtcctggac cagttcatgg
     4021 attcaatgca gttggatccc gagacggtgg acaaccttga tgcctacagc cacatcccac
     4081 cccagctcat ggagaagtgc gcggctctca gcgtccggcc cgacactgtc aggaaccttg
     4141 tacagtccat gcaagtgctg tcaggtgtgt tcacggatgt ggaggcttcc ctgaaggaca
     4201 tcagagatct gttggaggag gatgagctgc tagagcagaa gtttcaggag gcggtgggcc
     4261 aggcaggggc catctccatc acctccaagg ctgagctggc agaggtgagg cgagaatggg
     4321 ccaagtacat ggaagtccat gagaaggcct ccttcaccaa cagtgagctg caccgtgcca
     4381 tgaacctgca cgtcggcaac ctgcgcctgc tcagcgggcc gcttgaccag gtccgggctg
     4441 ccctgcccac accggccctc tccccagagg acaaggccgt gctgcaaaac ctaaagcgca
     4501 tcctggctaa ggtgcaggag atgcgggacc agcgcgtgtc cctggagcag cagctgcgtg
     4561 agcttatcca gaaagatgac atcactgcct cgctggtcac cacagaccac tcagagatga
     4621 agaagttgtt cgaggagcag ctgaaaaagt atgaccagct gaaggtgtac ctggagcaga
     4681 acctggccgc ccaggaccgt gtcctctgtg cactgacaga ggccaacgtg cagtacgcag
     4741 ccgtgcggcg ggtactcagc gacttggacc aaaagtggaa ctccacgctg cagaccctgg
     4801 tggcctcgta tgaagcctat gaggacctga tgaagaagtc gcaggagggc agggacttct
     4861 acgcagatct ggagagcaag gtggctgctc tgctggagcg cacgcagtcc acctgccagg
     4921 cccgcgaggc tgcccgccag cagctcctgg acagggagct gaagaagaag ccgccgccac
     4981 ggcccacagc cccaaagccg ctgctgcccc gcagggagga gagtgaggca gtggaagcag
     5041 gagacccccc tgaggagctg cgcagcctcc cccctgacat ggtggctggc ccacgactgc
     5101 ctgacacctt cctgggaagt gccaccccgc tccactttcc tcccagcccc ttccccagct
     5161 ccacaggccc aggaccccac tatctctcag gccccttgcc ccctggtacc tactcgggcc
     5221 ccacccagct gatacagccc agggccccag ggccccatgc aatgcccgta gcacctgggc
     5281 ctgccctcta cccagcccct gcctacacac cggagctggg ccttgtgccc cgatcctccc
     5341 cacagcatgg cgtggtgagc agtccctatg tgggggtagg gccggcccca ccagttgcag
     5401 gtctcccctc ggccccacct cctcaattct caggccccga gttggccatg gcggttcggc
     5461 cagccaccac cacagtagat agcatccagg cgcccatccc cagccacaca gccccacggc
     5521 caaaccccac ccctgctcct cccccgccct gcttccctgt gcccccaccg cagccactgc
     5581 ccacgcctta cacctaccct gcaggggcta agcaacccat cccggcacag caccacttct
     5641 cttctgggat ccccgcaggt tttccagccc caaggattgg gccccagccc cagccccatc
     5701 ctcagcccca tccttcacaa gcgtttgggc ctcagccccc acagcagccc cttccactcc
     5761 agcatccaca tctcttccca ccccaggccc caggactcct acccccacaa tccccctacc
     5821 cctatgcccc tcagcctggg gtcctggggc agccgccacc ccccctacac acccagctct
     5881 acccaggtcc cgctcaagac cctctgccag cccactcagg ggctctgcct ttccccagcc
     5941 ctgggccccc tcagcctccc catcccccac tggcatatgg tcctgcccct tctaccagac
     6001 ccatgggccc ccaggcagcc cctcttacca ttcgagggcc ctcgtctgct ggccagtcca
     6061 cccctagtcc ccacctggtg ccttcacctg ccccatctcc agggcctggt ccggtacccc
     6121 ctcgcccccc agcagcagaa ccaccccctt gcctgcgccg aggcgccgca gctgcagacc
     6181 tgctctcctc cagcccggag agccagcatg gcggcactca gtctcctggg ggtgggcagc
     6241 ccctgctgca gcccaccaag gtggatgcag ctgagggtcg tcggccgcag gccctgcggc
     6301 tgattgagcg ggacccctat gagcatcctg agaggctgcg gcagttgcag caggagctgg
     6361 aggcctttcg gggtcagctg ggggatgtgg gagctctgga cactgtctgg cgagagctgc
     6421 aagatgcgca ggaacatgat gcccgaggcc gttccatcgc cattgcccgc tgctactcac
     6481 tgaagaaccg gcaccaggat gtcatgccct atgacagtaa ccgtgtggtg ctgcgctcag
     6541 gcaaggatga ctacatcaat gccagctgcg tggaggggct ctccccatac tgccccccgc
     6601 tagtggcaac ccaggcccca ctgcctggca cagctgctga cttctggctc atggtccatg
     6661 agcagaaagt gtcagtcatt gtcatgctgg tttctgaggc tgagatggag aagcaaaaag
     6721 tggcacgcta cttccccacc gagaggggcc agcccatggt gcacggtgcc ctgagcctgg
     6781 cattgagcag cgtccgcagc accgaaaccc atgtggagcg cgtgctgagc ctgcagttcc
     6841 gagaccagag cctcaagcgc tctcttgtgc acctgcactt ccccacttgg cctgagttag
     6901 gcctgcccga cagccccagc aacttgctgc gcttcatcca ggaggtgcac gcacattacc
     6961 tgcatcagcg gccgctgcac acgcccatca ttgtgcactg cagctctggt gtgggccgca
     7021 cgggagcctt tgcactgctc tatgcagctg tgcaggaggt ggaggctggg aacggaatcc
     7081 ctgagctgcc tcagctggtg cggcgcatgc ggcagcagag aaagcacatg ctgcaggaga
     7141 agctgcacct caggttctgc tatgaggcag tggtgagaca cgtggagcag gtcctgcagc
     7201 gccatggtgt gcctcctcca tgcaaaccct tggccagtgc aagcatcagc cagaagaacc
     7261 accttcctca ggactcccag gacctggtcc tcggtgggga tgtgcccatc agctccatcc
     7321 aggccaccat tgccaagctc agcattcggc ctcctggggg gttggagtcc ccggttgcca
     7381 gcttgccagg ccctgcagag cccccaggcc tcccgccagc cagcctccca gagtctaccc
     7441 caatcccatc ttcctccccg cccccccttt cctccccact acctgaggct ccccagccta
     7501 aggaggagcc gccagtgcct gaagccccca gctcggggcc cccctcctcc tccctggaat
     7561 tgctggcctc cttgacccca gaggccttct ccctggacag ctccctgcgg ggcaaacagc
     7621 ggatgagcaa gcataacttt ctgcaggccc ataacgggca agggctgcgg gccacccggc
     7681 cctctgacga ccccctcagc cttctggatc cactctggac actcaacaag accggatccg
     7741 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
     7801 acaagtagtc tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     7861 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     7921 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     7981 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     8041 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     8101 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     8161 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     8221 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     8281 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     8341 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8401 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8461 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8521 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     8581 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8641 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8701 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8761 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     8821 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     8881 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     8941 ctgaccgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     9001 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     9061 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     9121 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     9181 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     9241 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     9301 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     9361 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     9421 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     9481 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     9541 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
     9601 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     9661 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     9721 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
     9781 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     9841 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     9901 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     9961 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
    10021 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
    10081 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
    10141 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
    10201 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    10261 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    10321 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    10381 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
    10441 tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc
    10501 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10561 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10621 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    10681 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10741 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10801 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10861 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10921 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10981 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    11041 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    11101 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    11161 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    11221 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    11281 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11341 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11401 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11461 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11521 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11581 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11641 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11701 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11761 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11821 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11881 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11941 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    12001 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    12061 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    12121 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    12181 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    12241 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    12301 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    12361 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    12421 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    12481 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    12541 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12601 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12661 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    12721 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12781 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    12841 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    12901 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    12961 ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt
    13021 actacaaact tagtagt
//
