LOCUS V046297 9841 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046297 VERSION V046297 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..4516 /label="NSRP1(NM_032141)" /note="NSRP1(NM_032141)" /gene="NSRP1" CDS 4523..4549 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4553..4579 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4583..4609 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4629..5128 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5149..5748 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5762..6350 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6557..7190 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7319..7335) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7343..7359 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7367..7397) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7412..7433 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7721..8309) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8480..9340) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9341..9445) /gene="bla" /label="AmpR promoter" polyA_signal 9493..9627 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggcgat tccgggcagg cagtatgggc ttattttgcc 2881 aaagaaaaca cagcagttgc accctgtttt gcaaaaacca tcagtgtttg ggaatgattc 2941 tgatgatgat gatgagacct ctgtgagtga aagccttcag agggaagctg ctaagaagca 3001 ggccatgaaa cagaccaaac tggaaatcca gaaggccctt gcagaagatg ctactgtgta 3061 tgaatatgac agtatttatg atgaaatgca gaaaaaaaag gaggaaaata atcccaaatt 3121 gcttttgggg aaagacagaa agcccaagta tattcacaac ttgctaaaag cagttgagat 3181 cagaaaaaag gaacaggaaa aaagaatgga aaagaaaata cagagagaac gagaaatgga 3241 aaagggggag tttgatgata aagaagcatt tgtgacatct gcatataaga aaaaactgca 3301 agagagagct gaagaagaag aaagagaaaa gagggctgct gcactggaag catgtttgga 3361 tgtaaccaag cagaaagatc tcagtggatt ttataggcac ctattaaatc aagcagttgg 3421 tgaagaggaa gtacctaaat gcagctttcg tgaagccaga tctggtataa aggaagaaaa 3481 atcaaggggc ttctccaatg aagtaagttc aaaaaacaga ataccacaag agaaatgcat 3541 tcttcaaact gatgtgaaag tagaggaaaa cccagatgca gacagtgact tcgatgctaa 3601 gagcagtgcg gatgatgaaa tagaagaaac tagagtgaac tgcagaaggg aaaaggtcat 3661 agagacccct gagaatgact tcaagcacca caggagtcaa aaccactctc ggtcacctag 3721 tgaagaaaga gggcacagta ccaggcacca cacgaaagga tcacgaacgt cgagaggaca 3781 tgagaaaagg gaagatcagc accagcagaa gcaatccaga gaccaagaga accattacac 3841 tgaccgtgat taccggaaag aaagggattc tcataggcac agagaggcca gtcatagaga 3901 ttcccattgg aagaggcatg aacaggaaga taaaccaagg gcgagggacc aaagagaaag 3961 aagtgacaga gtatggaaaa gggagaaaga tagggagaaa tattcccaaa gagaacaaga 4021 aagagataga caacaaaatg atcagaaccg acccagtgag aaaggagaga aggaagagaa 4081 aagcaaagca aaggaagagc atatgaaagt aaggaaggaa agatatgaaa ataatgataa 4141 atacagagat agagaaaaac gagaggtagg tgttcagtct tcagaaagaa atcaagacag 4201 aaaggaaagc agcccaaatt ctagggcaaa ggataaattt cttgaccaag aaagatccaa 4261 caaaatgaga aacatggcaa aggacaaaga aagaaaccaa gagaaaccct ctaattctga 4321 atcatcactg ggagcaaaac acagactcac agaggaaggg caagagaagg gtaaagaaca 4381 agagagacca cctgaggcag tgagcaagtt tgcaaagcgg aacaatgaag aaactgtaat 4441 gtcagctaga gacaggtact tggccaggca gatggcgcgg gttaatgcaa agacctatat 4501 tgagaaagaa gatgatggat cctacccata cgacgtacct gattatgcaa cttatcctta 4561 tgatgtacct gattatgcta gctatcctta tgatgtccca gactacgcat agtctagata 4621 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 4681 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 4741 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 4801 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 4861 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 4921 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 4981 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 5041 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 5101 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 5161 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5221 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 5281 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 5341 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5401 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5461 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5521 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 5581 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 5641 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 5701 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 5761 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5821 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5881 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 5941 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6001 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6061 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6121 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 6181 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6241 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6301 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 6361 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 6421 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 6481 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6541 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 6601 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 6661 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 6721 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 6781 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 6841 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 6901 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 6961 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 7021 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 7081 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 7141 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 7201 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 7261 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 7321 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7381 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7441 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7501 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7561 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7621 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7681 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7741 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7801 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7861 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7921 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7981 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 8041 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 8101 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 8161 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8221 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 8281 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8341 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8401 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8461 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8521 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8581 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8641 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8701 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8761 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8821 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8881 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8941 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9001 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 9061 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 9121 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9181 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9241 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9301 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9361 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9421 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 9481 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 9541 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 9601 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 9661 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 9721 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 9781 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 9841 t //