LOCUS V038279 12996 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038279 VERSION V038279 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2807..3514 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 3566..7789 /label="MET(NM_001127500)" /note="MET(NM_001127500)" /gene="MET" promoter 7831..8330 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 8351..8950 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 8964..9552 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 9759..10392 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(10521..10537) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 10545..10561 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(10569..10599) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 10614..10635 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10923..11511) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(11682..12542) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(12543..12647) /gene="bla" /label="AmpR promoter" polyA_signal 12695..12829 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg tgagcaaggg 2821 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 2881 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2941 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 3001 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 3061 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 3121 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 3181 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 3241 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 3301 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 3361 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 3421 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 3481 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatctc gagccggtgg 3541 aggcggatca ggaggcggtg gatctatgaa ggcccccgct gtgcttgcac ctggcatcct 3601 cgtgctcctg tttaccttgg tgcagaggag caatggggag tgtaaagagg cactagcaaa 3661 gtccgagatg aatgtgaata tgaagtatca gcttcccaac ttcaccgcgg aaacacccat 3721 ccagaatgtc attctacatg agcatcacat tttccttggt gccactaact acatttatgt 3781 tttaaatgag gaagaccttc agaaggttgc tgagtacaag actgggcctg tgctggaaca 3841 cccagattgt ttcccatgtc aggactgcag cagcaaagcc aatttatcag gaggtgtttg 3901 gaaagataac atcaacatgg ctctagttgt cgacacctac tatgatgatc aactcattag 3961 ctgtggcagc gtcaacagag ggacctgcca gcgacatgtc tttccccaca atcatactgc 4021 tgacatacag tcggaggttc actgcatatt ctccccacag atagaagagc ccagccagtg 4081 tcctgactgt gtggtgagcg ccctgggagc caaagtcctt tcatctgtaa aggaccggtt 4141 catcaacttc tttgtaggca ataccataaa ttcttcttat ttcccagatc atccattgca 4201 ttcgatatca gtgagaaggc taaaggaaac gaaagatggt tttatgtttt tgacggacca 4261 gtcctacatt gatgttttac ctgagttcag agattcttac cccattaagt atgtccatgc 4321 ctttgaaagc aacaatttta tttacttctt gacggtccaa agggaaactc tagatgctca 4381 gacttttcac acaagaataa tcaggttctg ttccataaac tctggattgc attcctacat 4441 ggaaatgcct ctggagtgta ttctcacaga aaagagaaaa aagagatcca caaagaagga 4501 agtgtttaat atacttcagg ctgcgtatgt cagcaagcct ggggcccagc ttgctagaca 4561 aataggagcc agcctgaatg atgacattct tttcggggtg ttcgcacaaa gcaagccaga 4621 ttctgccgaa ccaatggatc gatctgccat gtgtgcattc cctatcaaat atgtcaacga 4681 cttcttcaac aagatcgtca acaaaaacaa tgtgagatgt ctccagcatt tttacggacc 4741 caatcatgag cactgcttta ataggacact tctgagaaat tcatcaggct gtgaagcgcg 4801 ccgtgatgaa tatcgaacag agtttaccac agctttgcag cgcgttgact tattcatggg 4861 tcaattcagc gaagtcctct taacatctat atccaccttc attaaaggag acctcaccat 4921 agctaatctt gggacatcag agggtcgctt catgcaggtt gtggtttctc gatcaggacc 4981 atcaacccct catgtgaatt ttctcctgga ctcccatcca gtgtctccag aagtgattgt 5041 ggagcataca ttaaaccaaa atggctacac actggttatc actgggaaga agatcacgaa 5101 gatcccattg aatggcttgg gctgcagaca tttccagtcc tgcagtcaat gcctctctgc 5161 cccacccttt gttcagtgtg gctggtgcca cgacaaatgt gtgcgatcgg aggaatgcct 5221 gagcgggaca tggactcaac agatctgtct gcctgcaatc tacaaggttt tcccaaatag 5281 tgcacccctt gaaggaggga caaggctgac catatgtggc tgggactttg gatttcggag 5341 gaataataaa tttgatttaa agaaaactag agttctcctt ggaaatgaga gctgcacctt 5401 gactttaagt gagagcacga tgaatacatt gaaatgcaca gttggtcctg ccatgaataa 5461 gcatttcaat atgtccataa ttatttcaaa tggccacggg acaacacaat acagtacatt 5521 ctcctatgtg gatcctgtaa taacaagtat ttcgccgaaa tacggtccta tggctggtgg 5581 cactttactt actttaactg gaaattacct aaacagtggg aattctagac acatttcaat 5641 tggtggaaaa acatgtactt taaaaagtgt gtcaaacagt attcttgaat gttatacccc 5701 agcccaaacc atttcaactg agtttgctgt taaattgaaa attgacttag ccaaccgaga 5761 gacaagcatc ttcagttacc gtgaagatcc cattgtctat gaaattcatc caaccaaatc 5821 ttttattagt acttggtgga aagaacctct caacattgtc agttttctat tttgctttgc 5881 cagtggtggg agcacaataa caggtgttgg gaaaaacctg aattcagtta gtgtcccgag 5941 aatggtcata aatgtgcatg aagcaggaag gaactttaca gtggcatgtc aacatcgctc 6001 taattcagag ataatctgtt gtaccactcc ttccctgcaa cagctgaatc tgcaactccc 6061 cctgaaaacc aaagcctttt tcatgttaga tgggatcctt tccaaatact ttgatctcat 6121 ttatgtacat aatcctgtgt ttaagccttt tgaaaagcca gtgatgatct caatgggcaa 6181 tgaaaatgta ctggaaatta agggaaatga tattgaccct gaagcagtta aaggtgaagt 6241 gttaaaagtt ggaaataaga gctgtgagaa tatacactta cattctgaag ccgttttatg 6301 cacggtcccc aatgacctgc tgaaattgaa cagcgagcta aatatagagt ggaagcaagc 6361 aatttcttca accgtccttg gaaaagtaat agttcaacca gatcagaatt tcacaggatt 6421 gattgctggt gttgtctcaa tatcaacagc actgttatta ctacttgggt ttttcctgtg 6481 gctgaaaaag agaaagcaaa ttaaagatct gggcagtgaa ttagttcgct acgatgcaag 6541 agtacacact cctcatttgg ataggcttgt aagtgcccga agtgtaagcc caactacaga 6601 aatggtttca aatgaatctg tagactaccg agctactttt ccagaagatc agtttcctaa 6661 ttcatctcag aacggttcat gccgacaagt gcagtatcct ctgacagaca tgtcccccat 6721 cctaactagt ggggactctg atatatccag tccattactg caaaatactg tccacattga 6781 cctcagtgct ctaaatccag agctggtcca ggcagtgcag catgtagtga ttgggcccag 6841 tagcctgatt gtgcatttca atgaagtcat aggaagaggg cattttggtt gtgtatatca 6901 tgggactttg ttggacaatg atggcaagaa aattcactgt gctgtgaaat ccttgaacag 6961 aatcactgac ataggagaag tttcccaatt tctgaccgag ggaatcatca tgaaagattt 7021 tagtcatccc aatgtcctct cgctcctggg aatctgcctg cgaagtgaag ggtctccgct 7081 ggtggtccta ccatacatga aacatggaga tcttcgaaat ttcattcgaa atgagactca 7141 taatccaact gtaaaagatc ttattggctt tggtcttcaa gtagccaaag gcatgaaata 7201 tcttgcaagc aaaaagtttg tccacagaga cttggctgca agaaactgta tgctggatga 7261 aaaattcaca gtcaaggttg ctgattttgg tcttgccaga gacatgtatg ataaagaata 7321 ctatagtgta cacaacaaaa caggtgcaaa gctgccagtg aagtggatgg ctttggaaag 7381 tctgcaaact caaaagttta ccaccaagtc agatgtgtgg tcctttggcg tgctcctctg 7441 ggagctgatg acaagaggag ccccacctta tcctgacgta aacacctttg atataactgt 7501 ttacttgttg caagggagaa gactcctaca acccgaatac tgcccagacc ccttatatga 7561 agtaatgcta aaatgctggc accctaaagc cgaaatgcgc ccatcctttt ctgaactggt 7621 gtcccggata tcagcgatct tctctacttt cattggggag cactatgtcc atgtgaacgc 7681 tacttatgtg aacgtaaaat gtgtcgctcc gtatccttct ctgttgtcat cagaagataa 7741 cgctgatgat gaggtggaca cacgaccagc ctccttctgg gagacatcat agggatccac 7801 cggatctaga taactgatca taattctacc gggtagggga ggcgcttttc ccaaggcagt 7861 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc 7921 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct 7981 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt 8041 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca 8101 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct 8161 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc 8221 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa 8281 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 8341 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 8401 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 8461 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 8521 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 8581 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 8641 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 8701 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 8761 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 8821 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 8881 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 8941 cggtgcctga ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga 9001 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 9061 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 9121 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 9181 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 9241 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 9301 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 9361 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 9421 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 9481 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 9541 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac 9601 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga 9661 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc 9721 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca 9781 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta 9841 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa 9901 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt 9961 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag 10021 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa 10081 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg 10141 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt 10201 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 10261 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 10321 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 10381 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 10441 aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc 10501 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 10561 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 10621 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 10681 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 10741 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 10801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 10861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 10921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 10981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 11041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 11101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 11161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 11221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 11281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 11341 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 11401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 11461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 11521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 11581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 11641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 11701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 11761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 11821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 11881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 11941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 12001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 12061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 12121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 12181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 12241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 12301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 12361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 12421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 12481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 12541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 12601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 12661 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg 12721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 12781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac 12841 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta 12901 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 12961 ttgtatttgt taaatatgta ctacaaactt agtagt //