LOCUS       V047077                10402 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047077
VERSION     V047077
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5077
                     /label="JUP(NM_002230)"
                     /note="JUP(NM_002230)"
                     /gene="JUP"
     CDS             5084..5110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5114..5140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5144..5170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5190..5689
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5710..6309
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6323..6911
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7118..7751
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7880..7896)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7904..7920
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7928..7958)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7973..7994
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8282..8870)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9041..9901)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9902..10006)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10054..10188
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggaggt gatgaacctg atggagcagc ctatcaaggt
     2881 gactgagtgg cagcagacat acacctacga ctcgggtatc cactcgggcg ccaacacctg
     2941 cgtgccctcc gtcagcagca agggcatcat ggaggaggat gaggcctgcg ggcgccagta
     3001 cacgctcaag aaaaccacca cttacaccca gggggtgccc cccagccaag gtgatctgga
     3061 gtaccagatg tccacaacag ccagggccaa acgggtgcgg gaggccatgt gccctggtgt
     3121 gtcaggcgag gacagctcgc ttctgctggc cacccaggtg gaggggcagg ccaccaacct
     3181 gcagcgactg gccgagccgt cccagctgct caagtcggcc attgtgcatc tcatcaacta
     3241 ccaggacgat gccgagctgg ccactcgcgc cctgcccgag ctcaccaaac tgctcaacga
     3301 cgaggacccg gtggtggtga ccaaggcggc catgattgtg aaccagctgt cgaagaagga
     3361 ggcgtcgcgg cgggccctga tgggctcgcc ccagctggtg gccgctgtcg tgcgtaccat
     3421 gcagaatacc agcgacctgg acacagcccg ctgcaccacc agcatcctgc acaacctctc
     3481 ccaccaccgg gaggggctgc tcgccatctt caagtcgggt ggcatccctg ctctggtccg
     3541 catgctcagc tcccctgtgg agtcggtcct gttctatgcc atcaccacgc tgcacaacct
     3601 gctcctgtac caggagggcg ccaagatggc cgtgcgcctg gccgacgggc tgcaaaagat
     3661 ggtgcccctg ctcaacaaga acaaccccaa gttcctggcc atcaccaccg actgcctgca
     3721 gctcctggcc tacggcaacc aggagagcaa gctgatcatc ctggccaatg gtgggcccca
     3781 ggccctcgtg cagatcatgc gtaactacag ttatgaaaag ctgctctgga ccaccagtcg
     3841 tgtgctcaag gtgctatccg tgtgtcccag caataagcct gccattgtgg aggctggtgg
     3901 gatgcaggcc ctgggcaagc acctgaccag caacagcccc cgcctggtgc agaactgcct
     3961 gtggaccctg cgcaacctct cagatgtggc caccaagcag gagggcctgg agagtgtgct
     4021 gaagattctg gtgaatcagc tgagtgtgga tgacgtcaac gtcctcacct gtgccacggg
     4081 cacactctcc aacctgacat gcaacaacag caagaacaag acgctggtga cacagaacag
     4141 cggtgtggag gctctcatcc atgccatcct gcgtgctggt gacaaggacg acatcacgga
     4201 gcctgccgtc tgcgctctgc gccacctcac tagccgccac cctgaggccg agatggccca
     4261 gaactctgtg cgtctcaact atggcatccc agccatcgtg aagctgctca accagcccaa
     4321 ccagtggcca ctggtcaagg caaccatcgg cttgatcagg aatctggccc tgtgcccagc
     4381 caaccatgcc ccgctgcagg aggcagcggt catcccccgc ctcgtccaac tgctggtgaa
     4441 ggcccaccag gatgcccagc gccacgtagc tgcaggcaca cagcagccct acacggatgg
     4501 tgtgaggatg gaggagattg tggagggctg caccggagca ctgcacatcc tcgcccggga
     4561 ccccatgaac cgcatggaga tcttccggct caacaccatt cccctgtttg tgcagctcct
     4621 gtactcgtcg gtggagaaca tccagcgcgt ggctgccggg gtgctgtgtg agctggccca
     4681 ggacaaggag gcggccgacg ccattgatgc agagggggcc tcggccccac tcatggagtt
     4741 gctgcactcc cgcaacgagg gcactgccac ctacgctgct gccgtcctgt tccgcatctc
     4801 cgaggacaag aacccagact accggaagcg cgtgtccgtg gagctcacca actccctctt
     4861 caagcatgac ccggctgcct gggaggctgc ccagagcatg attcccatca atgagcccta
     4921 tggagatgac atggatgcca cctaccgccc catgtactcc agcgatgtgc cccttgaccc
     4981 gctggagatg cacatggaca tggatggaga ctaccccatc gacacctaca gcgacggcct
     5041 caggcccccg taccccactg cagaccacat gctggccgga tcctacccat acgacgtacc
     5101 tgattatgca acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc
     5161 agactacgca tagtctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     5221 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     5281 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     5341 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     5401 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     5461 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     5521 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     5581 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     5641 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     5701 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     5761 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     5821 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     5881 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     5941 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6001 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6061 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6121 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6181 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6241 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6301 ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
     6361 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6421 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6481 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6541 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6601 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6661 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     6721 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6781 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6841 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6901 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
     6961 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     7021 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     7081 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     7141 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     7201 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     7261 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     7321 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     7381 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     7441 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     7501 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     7561 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7621 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7681 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7741 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7801 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct
     7861 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7921 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7981 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8041 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8101 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8701 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8941 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9001 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9061 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9121 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9181 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9241 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9301 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9361 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9421 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9481 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9541 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9601 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9661 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9721 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9781 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9841 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9901 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9961 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10021 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
    10081 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10141 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    10201 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    10261 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    10321 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa
    10381 tatgtactac aaacttagta gt
//
