LOCUS       V018316                12193 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018316
VERSION     V018316
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..6949
                     /label="Igf1r(NM_010513)"
                     /note="Igf1r(NM_010513)"
                     /gene="Igf1r"
     promoter        6981..7480
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7501..8100
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8114..8702
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8909..9542
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9671..9687)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9695..9711
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9719..9749)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9764..9785
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10073..10661)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10832..11692)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11693..11797)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11845..11979
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgaagtc tggctccgga ggagggtccc cgacctcgct
     2881 gtgggggctc gtgtttctct ccgccgcgct ctctctctgg ccgacgagtg gagaaatctg
     2941 tgggcccggc attgacatcc gcaacgacta tcagcagctg aagcgcctgg aaaactgcac
     3001 ggtgatcgag ggcttcctcc acatcctgct catctccaag gccgaggact accgaagcta
     3061 ccgcttcccc aagctcaccg tcatcactga gtacttgctg ctcttccgag tcgctggcct
     3121 cgagagcctg ggagacctct tccccaacct cacagtcatc cgtggctgga aactcttcta
     3181 caactacgca ctggtcatct tcgagatgac caatctcaag gatattgggc tttataatct
     3241 gaggaacatt actcgggggg ccatcaggat tgagaagaac gccgacctct gttacctctc
     3301 caccatagac tggtctctca tcttggatgc ggtgtccaat aactacattg tggggaacaa
     3361 gcccccgaag gaatgtgggg acctgtgtcc agggacattg gaggagaagc ccatgtgtga
     3421 gaagaccacc atcaacaatg agtacaacta ccgctgctgg accacaaatc gctgccagaa
     3481 aatgtgccca agtgtgtgcg ggaagcgagc ctgcaccgag aacaacgagt gctgccaccc
     3541 ggagtgcctg ggcagctgcc acacaccgga cgacaacaca acctgcgtgg cctgcagaca
     3601 ctactactac aaaggcgtgt gtgtgcctgc ctgcccgcct ggcacctaca ggttcgaggg
     3661 ctggcgctgt gtggatcgcg atttctgcgc caacatcccc aacgctgaga gcagtgactc
     3721 ggatggcttc gttatccacg acgatgagtg catgcaggag tgtccctcag gcttcatccg
     3781 caacagcacc cagagcatgt actgtatccc ctgcgaaggc ccctgcccca aagtctgcgg
     3841 cgatgaagag aagaaaacga aaaccatcga ttcggtgact tctgctcaaa tgctccaagg
     3901 atgcaccatc ctgaagggca atctgcttat taacatccgg agaggcaata acattgcctc
     3961 ggagttggag aacttcatgg ggctcatcga ggtggtgacc ggctacgtga agatccgcca
     4021 ttctcatgcc ttggtctcct tgtccttcct gaagaacctt cgtctcatct taggagagga
     4081 gcagctggaa gggaactact ccttctatgt cctagacaac cagaacttgc agcagctgtg
     4141 ggactggaac caccggaacc tgaccgtcag gtccggaaag atgtactttg ctttcaatcc
     4201 caagctgtgt gtctccgaaa tttaccgcat ggaggaagtg accggaacca agggacgcca
     4261 gagcaaaggg gacataaaca ccaggaacaa cggagagcga gcttcctgtg aaagtgatgt
     4321 tctccgtttc acctccacca cgacctggaa gaaccgaatc atcataacgt ggcaccggta
     4381 ccggccgccg gactaccggg atctcatcag cttcacagtt tactacaagg aggcaccatt
     4441 taaaaacgtt acggaatatg acgggcagga tgcctgtggc tccaacagct ggaacatggt
     4501 ggatgtagac ctgcctccga acaaggaggg cgagcctggc attttactgc atgggctgaa
     4561 gccctggacc cagtatgctg tctatgtcaa ggctgtgacc ctcaccatgg tggaaaacga
     4621 ccatatccgt ggggccaaaa gtgaaatctt gtacattcgc accaatgctt cagtcccttc
     4681 cattccccta gatgtcctct cagcatcaaa ctcttcctct cagctgattg tgaagtggaa
     4741 tcctccaact ctgcccaatg gtaacttgag ttactacatt gtgaggtggc agcggcagcc
     4801 ccaggatggt tacctgtacc ggcacaacta ctgctccaaa gacaaaatac ccatcagaaa
     4861 gtacgccgat ggtaccatcg acgtggagga ggtgacggaa aatcccaaga cagaagtgtg
     4921 tggtggtgat aaagggccat gctgcgcttg ccctaaaact gaagctgaga agcaggctga
     4981 gaaggaggag gctgagtacc gtaaagtctt tgagaatttc cttcacaatt ccatctttgt
     5041 gcccaggccc gaaaggaggc ggagagacgt catgcaagtg gccaacacga ccatgtccag
     5101 ccgaagcagg aacaccacgg tagctgacac ctacaatatc acagacccgg aggagttcga
     5161 gacagagtac cctttctttg agagcagagt ggataacaag gagaggactg tcatctccaa
     5221 cctccggcct ttcactctgt accgcatcga tatccacagc tgcaaccacg aggctgagaa
     5281 gctgggctgc agcgcctcca acttcgtctt tgcgagaacc atgccagcag aaggagcaga
     5341 tgatatccct ggtccggtga cctgggagcc aagacccgaa aactccatct ttttaaagtg
     5401 gccagaaccc gagaacccca acggattgat cctaatgtat gaaattaaat acgggtcgca
     5461 agtcgaggat cagcgggaat gtgtgtccag acaggagtac aggaagtacg gaggggccaa
     5521 actcaaccgt ctaaacccag ggaactatac agcccggatt caggctacct ccctctctgg
     5581 gaatgggtca tggacagatc ctgtgttctt ctatgtcccc gccaaaacga cgtatgagaa
     5641 cttcatgcat ctgatcattg ctctgccggt tgccatcctg ctgatcgttg gggggctggt
     5701 tatcatgctg tatgtcttcc atagaaagag aaataacagc aggttgggca atggagtgct
     5761 gtatgcttct gtgaaccccg agtatttcag cgcagctgat gtgtacgtgc ctgatgaatg
     5821 ggaggtagct cgagagaaga tcaccatgaa ccgggagctc ggacaagggt cctttgggat
     5881 ggtctatgaa ggagtggcca agggtgtggt caaggatgaa cccgaaacca gagtggccat
     5941 caagacggta aacgaggctg caagtatgcg tgaaagaatc gagtttctca acgaggcctc
     6001 ggtgatgaag gagttcaatt gtcaccatgt ggtccggttg ctgggtgtgg tatcccaagg
     6061 ccagcccacc ctggtcatca tggaactaat gacacgcggt gatctcaaaa gttatctccg
     6121 gtctctgagg ccagaagtgg agcagaataa tctagtcctc attcctccga gcttaagcaa
     6181 gatgatccag atggctggag agattgcaga tggcatggcc tacctcaatg ccaacaagtt
     6241 cgtccacaga gaccttgctg ctaggaactg catggtagcc gaagatttca cagtcaaaat
     6301 tggagatttc ggtatgacac gagacatcta cgagacggac tactaccgga aaggcgggaa
     6361 ggggttgctg cctgtgcgct ggatgtctcc cgagtccctc aaggatggtg tcttcactac
     6421 tcattctgat gtctggtcct tcggggtcgt cctctgggag atcgccacgc tggctgagca
     6481 gccctaccag ggcttgtcca acgagcaagt tcttcgtttc gtcatggagg gtggccttct
     6541 ggacaagccg gacaactgcc ctgatatgct gtttgaactt atgcgcatgt gctggcagta
     6601 taaccccaag atgcggccct ccttcctgga gatcatcggc agcatcaagg atgagatgga
     6661 gcccagcttc caggaggtct ccttctacta cagcgaggag aacaagcctc ccgagccaga
     6721 ggagctggag atggagcctg agaacatgga gagcgtccca ctggaccctt cggcctcctc
     6781 agcctccctg cctctgcctg aaagacactc aggacacaag gctgagaatg gcccgggccc
     6841 tggcgtgctc gttctccgcg ccagttttga tgagagacag ccttacgctc acatgaacgg
     6901 gggacgcgcc aacgagaggg ccttgcctct gccccagtcc tcgacctgct gaggatcccg
     6961 cgactctaga taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     7021 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     7081 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     7141 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     7201 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     7261 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     7321 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     7381 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     7441 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     7501 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     7561 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     7621 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     7681 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     7741 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     7801 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     7861 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     7921 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     7981 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     8041 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     8101 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     8161 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8221 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     8281 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     8341 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     8401 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     8461 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     8521 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     8581 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     8641 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     8701 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     8761 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     8821 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8881 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     8941 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     9001 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     9061 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     9121 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     9181 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     9241 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     9301 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     9361 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9541 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     9601 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg
     9661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11761 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11821 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
    11881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    11941 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    12001 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    12061 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    12121 taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta
    12181 caaacttagt agt
//
