LOCUS V018565 12317 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018565 VERSION V018565 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2827..6363 /label="ITGA1(NM_181501)" /note="ITGA1(NM_181501)" /gene="ITGA1" CDS 6415..7125 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" promoter 7152..7651 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 7672..8271 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 8285..8873 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 9080..9713 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(9842..9858) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9866..9882 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9890..9920) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9935..9956 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10244..10832) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(11003..11863) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11864..11968) /gene="bla" /label="AmpR promoter" polyA_signal 12016..12150 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgg cccctcggcc ccgcgcccgc ccaggggtcg ctgtcgcctg ctgctggctc 2881 ctcactgttg ttctacgctg ctgcgtatca ttcaatgttg atgtgaaaaa ttcaatgact 2941 ttcagcggcc cggtggaaga catgtttgga tatactgttc aacaatatga aaatgaagaa 3001 ggaaaatggg tgcttattgg ttctccgtta gttggccaac ccaaaaacag aactggagat 3061 gtctataagt gtccagttgg gagaggtgaa tcattacctt gtgtaaagtt ggatctacca 3121 gttaatacat caattcccaa tgtcacagaa gtaaaggaga acatgacatt tggatcaact 3181 ttagtcacca acccaaatgg aggatttctg gcttgtgggc ccttatatgc ctatagatgt 3241 ggacatttgc attacacaac tggaatctgt tctgacgtca gccccacatt tcaagtcgtg 3301 aattccattg cccctgtaca agaatgcagc actcaactgg acatagtcat agtgctggat 3361 ggttccaaca gtatttaccc atgggacagt gttacagctt ttttaaatga ccttcttgaa 3421 agaatggata ttggtcctaa acagacacag gttggaattg tacagtatgg agaaaacgtg 3481 acccatgagt tcaacctcaa taagtattct tccaccgaag aggtacttgt tgcagcaaag 3541 aaaatagtcc agagaggtgg ccgccagact atgacagctc ttggaataga cacagcaaga 3601 aaggaggcat tcacggaagc ccggggtgcc cgaagaggag ttaaaaaagt catggttatt 3661 gtgacagatg gagagtctca tgacaatcat cgactgaaga aggtcatcca agactgtgaa 3721 gatgaaaaca ttcaacggtt ttccatagct attcttggca gctataaccg aggaaattta 3781 agcactgaaa aatttgtgga ggaaataaaa tcaattgcaa gtgaacccac tgaaaagcat 3841 ttcttcaatg tctctgatga attggctcta gtcaccattg ttaaaactct gggagaaaga 3901 atatttgccc tggaagccac agctgaccag tcagcagctt catttgaaat ggaaatgtct 3961 cagactggct tcagtgctca ttattcacag gactgggtca tgcttggagc agtaggagcc 4021 tatgattgga atggaacagt tgtcatgcag aaggctagtc aaatcataat ccctcgaaac 4081 acaaccttta atgttgagtc taccaaaaag aatgaaccgc ttgcttctta tttaggttac 4141 actgtaaact ctgctactgc ttcttctgga gatgtgctct atattgctgg acagcctcgg 4201 tacaatcata caggccaggt cattatctac aggatggaag atggaaacat caaaattctc 4261 cagacgctca gtggagaaca gattggttcc tactttggca gtattttaac aacaactgac 4321 attgacaagg attctaatac tgacattctt ctagtcggag cccctatgta catgggaaca 4381 gagaaggagg agcaaggaaa agtgtatgtg tatgctctca atcagacaag gtttgaatat 4441 caaatgagcc tggaacctat taagcagacg tgctgttcat ctcggcagca caattcatgc 4501 acaacagaaa acaaaaatga gccatgcggg gctcgttttg gaactgcaat tgctgctgta 4561 aaagacctca atcttgatgg atttaatgac atcgtgatag gagctccgct ggaagatgat 4621 cacgggggag ctgtgtacat ttatcatgga agtggcaaga ctataaggaa agagtatgca 4681 caacgtattc catcaggtgg ggatggtaag acactgaaat tttttggcca gtctatccac 4741 ggagaaatgg atttaaatgg tgacggtctg acagatgtga ctattggggg ccttggtggt 4801 gctgccctct tctggtcccg agatgtggcc gtagttaaag tgaccatgaa ttttgagcca 4861 aataaagtga atattcaaaa gaaaaactgc catatggagg gaaaggaaac agtatgcata 4921 aatgctacag tgtgttttga tgtgaaatta aagtctaaag aagacacgat ttatgaagct 4981 gatttgcagt accgtgtcac cctagattca ctaagacaaa tatcacgaag ttttttctct 5041 ggaactcaag agagaaaggt tcaaaggaac atcacagttc gaaaatcaga atgcactaag 5101 cactccttct acatgttgga caagcatgac tttcaggact ctgtgagaat aacgttggac 5161 tttaatctta ccgatccaga aaatgggcct gttcttgatg attctctacc aaactcagta 5221 catgaatata ttccctttgc caaagattgt ggaaataagg aaaaatgtat ctcagacctc 5281 agcctgcatg tcgccaccac tgaaaaggac ctgctgattg tccgatccca gaatgataag 5341 ttcaacgtta gcctcacagt caaaaataca aaggacagtg cctataacac caggacaata 5401 gtgcattatt ctccaaatct agttttttca ggaattgagg ctatccaaaa agacagttgt 5461 gaatctaatc ataatatcac atgtaaagtt ggatatccct tcctgagaag aggagagatg 5521 gtaactttca aaatattgtt tcagtttaac acatcctatc tcatggaaaa tgtgaccatt 5581 tatttaagtg caacaagtga cagcgaagaa cctcctgaaa ccctttctga taatgtagta 5641 aacatttcta tcccggtaaa atatgaagtt ggactacagt tttacagctc tgcaagtgaa 5701 taccacattt caattgctgc caatgagaca gtccctgaag ttattaattc tactgaggac 5761 attggaaatg aaattaatat cttctacttg attagaaaaa gtggatcttt tccaatgcca 5821 gagcttaagc tgtcaatttc attccccaat atgacatcaa atggttaccc tgtgctgtac 5881 ccaactggat tgtcatcttc tgagaatgca aactgcagac cccatatctt tgaggatcct 5941 ttcagtatca actctggaaa gaaaatgact acatcaactg accatctcaa acgaggcaca 6001 attctggact gcaatacatg taaatttgct accatcacat gtaatctcac ttcttctgac 6061 atcagccaag tcaatgtttc gcttatcttg tggaaaccaa cttttataaa atcatatttt 6121 tccagcttaa atcttactat aaggggagaa cttcggagtg aaaatgcatc tctggtttta 6181 agtagcagca atcaaaaaag agagcttgct attcaaatat ccaaagatgg gctaccgggc 6241 agagtgccat tatgggtcat cctgctgagt gcttttgccg gattgttgct gttaatgctg 6301 ctcattttag cactgtggaa gattggattc ttcaaaagac cactgaaaaa gaaaatggag 6361 aaacgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg 6421 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac 6481 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 6541 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc 6601 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc 6661 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 6721 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag 6781 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag 6841 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg 6901 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc 6961 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc 7021 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc 7081 gagggccgcc actccaccgg cggcatggac gagctgtaca agtagcggcc gcgactctag 7141 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 7201 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 7261 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 7321 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 7381 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 7441 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 7501 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 7561 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 7621 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 7681 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 7741 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 7801 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 7861 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 7921 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 7981 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 8041 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 8101 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 8161 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 8221 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg 8281 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 8341 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 8401 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 8461 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 8521 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 8581 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 8641 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 8701 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 8761 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 8821 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 8881 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 8941 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 9001 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 9061 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 9121 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 9181 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 9241 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 9301 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 9361 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 9421 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 9481 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 9541 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 9601 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 9661 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 9721 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 9781 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 9841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 9901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 9961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 10021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 10081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 10141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 10201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 10261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 10321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 10381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 10441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 10501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 10561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 10621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 10681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 10741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 10801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 10861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 10921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 10981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 11041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 11101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 11161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 11221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 11281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 11341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 11401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 11461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 11521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 11581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 11641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 11701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 11761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 11821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 11881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 11941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 12001 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 12061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 12121 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 12181 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 12241 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 12301 actacaaact tagtagt //