LOCUS V039872 10474 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039872 VERSION V039872 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5230 /label="IRF2BPL(NM_024496)" /note="IRF2BPL(NM_024496)" /gene="IRF2BPL" promoter 5262..5761 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5782..6381 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6395..6983 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7190..7823 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7952..7968) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7976..7992 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8000..8030) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8045..8066 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8354..8942) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9113..9973) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9974..10078) /gene="bla" /label="AmpR promoter" polyA_signal 10126..10260 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgtcggc ggcgcaggtg tcctcgtccc ggagacaatc 2881 ttgctacctg tgcgacctgc cccgcatgcc ctgggccatg atctgggact tctcggaacc 2941 cgtatgccgc ggttgcgtca actacgaggg cgctgatcgc atcgaattcg tgatcgagac 3001 agcgcgccag ctgaagcggg cgcacggctg cttccaggac ggccgctccc ccgggccgcc 3061 gccgcccgtc ggggtcaaga cagtggccct gtcggctaag gaagcggcgg cggcggcggc 3121 agcagcggcg gccgccgccg ccgccgcgca acagcaacag caacagcagc agcagcagca 3181 gcaacagcag cagcagcagc agcagcagca gcagcaacaa cagctcaacc acgttgatgg 3241 ttccagcaag cctgcggtgc tggcggcccc gtctggcctg gagcgctacg gcctaagcgc 3301 tgccgccgcc gccgccgccg ccgccgccgc tgcggtggaa cagcgcagcc gcttcgagta 3361 cccgccaccg ccggtgagcc tgggaagcag cagccacacc gcgcgactgc ccaacggcct 3421 ggggggccca aacggcttcc ccaaaccaac accagaggag ggacccccag agctgaaccg 3481 tcagagcccc aattcttctt cagcggcggc gtcggtggcg tctcggcgtg gaacgcacgg 3541 tgggctggtt acggggctgc ccaacccggg gggtggcgga ggcccccagc tcaccgtgcc 3601 ccccaacctg ctaccgcaga cgctgcttaa cggcccggcc agcgctgcgg tactcccccc 3661 accccctccc cacgccctgg gcagccgtgg gcccccgacg cctgctcccc caggggctcc 3721 tgggggcccc gcttgtctcg ggggtacccc gggtgtatcg gccacgtcgt cctccgcgtc 3781 gtcttcgacc tcttcgtcgg tggcagaggt gggcgtgggt gctggtggta agaggcccgg 3841 ctcggtgtcg agcacagacc aggagcgcga gttgaaggag aagcagcgca acgccgaggc 3901 cctggccgag ctgagcgaga gcctgcgcaa ccgcgccgag gagtgggcca gcaagcccaa 3961 gatggtccgc gacacgctgc tcacgctggc aggctgcacg ccctacgagg ttcgcttcaa 4021 gaaggaccac tcgctgctgg gccgcgtttt cgccttcgac gccgtctcca agcccggcat 4081 ggactacgaa ttgaagctgt tcattgagta ccccacgggc tcgggcaacg tgtactccag 4141 tgcatctggt gtggccaagc agatgtatca ggactgcatg aaggacttcg gccggggcct 4201 atcctcgggt ttcaagtacc tggagtacga aaagaagcac ggctccgggg actggcgcct 4261 gcttggagac ctgctccccg aagccgtgcg cttcttcaag gagggcgtgc ccggcgccga 4321 catgctgccc cagccctacc tggacgccag ctgtcccatg ctgcccactg ctctggtgag 4381 tctgagccgc gcccccagcg cacccccggg gaccggggcc ttgccgcccg ccgcgccgtc 4441 gggccggggc gcagccgcca gcctgcgcaa gagaaaggcc tctccggagc ccccggactc 4501 agccgagggc gcgctgaagc tgggcgagga acagcagagg cagcagtgga tggcgaacca 4561 gagcgaggcg ctgaagctca ccatgtccgc cgggggcttc gcggcgccgg ggcacgcggc 4621 ggggggtccg cctccgccgc ccccacctct gggaccccat tccaaccgga ccaccccacc 4681 tgagtcagcc ccccagaacg gtccgtcccc tatggccgct ctcatgtcgg tggcagatac 4741 tctgggcaca gcgcactcgc ccaaggatgg cagttccgtg cactctacca ctgcgtcggc 4801 gcggcgaaac agcagcagcc cagtctcgcc ggcctccgtg ccggggcagc gccgcttggc 4861 atcacgtaac ggggacctga atttacaggt ggcgcccccg ccgcctagcg cccacccggg 4921 catggaccaa gtgcaccccc aaaacattcc ggattccccc atggccaaca gcggacccct 4981 ctgctgcacc atttgccacg aacgtttgga ggatacgcat ttcgttcagt gcccttccgt 5041 ccccagccac aaattttgct tcccttgctc tagagagagt atcaaggccc agggggccac 5101 cggcgaggtg tattgcccca gcggagagaa atgcccccta gtcgggtcga atgtaccttg 5161 ggccttcatg cagggcgaaa tcgcgactat cttagctggg gatgttaaag tgaaaaagga 5221 gagagaccct tgaggatccc gcgactctag ataattctac cgggtagggg aggcgctttt 5281 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag 5341 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt 5401 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc 5461 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca 5521 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca 5581 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct 5641 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg 5701 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc 5761 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc 5821 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 5881 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 5941 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 6001 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 6061 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 6121 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 6181 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 6241 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 6301 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 6361 acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg 6421 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 6481 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 6541 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 6601 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 6661 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 6721 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 6781 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 6841 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6901 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 6961 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat 7021 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 7081 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 7141 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta 7201 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 7261 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga 7321 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag 7381 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg 7441 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg 7501 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt 7561 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa 7621 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct 7681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7801 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7861 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt 7921 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 7981 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 8041 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 8101 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 8161 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 8221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 8281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 8341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 8401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 8461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 8521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 8581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 8641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 8701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 8761 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 8821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 8881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 8941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 9001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 9061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 9121 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 9181 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 9241 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 9301 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 9361 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 9421 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 9481 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 9541 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 9601 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 9661 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 9721 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 9781 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 9841 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 9901 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 9961 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 10021 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 10081 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca 10141 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 10201 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 10261 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc 10321 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca 10381 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt 10441 gtatttgtta aatatgtact acaaacttag tagt //