LOCUS       V039872                10474 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039872
VERSION     V039872
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5230
                     /label="IRF2BPL(NM_024496)"
                     /note="IRF2BPL(NM_024496)"
                     /gene="IRF2BPL"
     promoter        5262..5761
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5782..6381
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6395..6983
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7190..7823
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7952..7968)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7976..7992
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8000..8030)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8045..8066
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8354..8942)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9113..9973)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9974..10078)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10126..10260
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgtcggc ggcgcaggtg tcctcgtccc ggagacaatc
     2881 ttgctacctg tgcgacctgc cccgcatgcc ctgggccatg atctgggact tctcggaacc
     2941 cgtatgccgc ggttgcgtca actacgaggg cgctgatcgc atcgaattcg tgatcgagac
     3001 agcgcgccag ctgaagcggg cgcacggctg cttccaggac ggccgctccc ccgggccgcc
     3061 gccgcccgtc ggggtcaaga cagtggccct gtcggctaag gaagcggcgg cggcggcggc
     3121 agcagcggcg gccgccgccg ccgccgcgca acagcaacag caacagcagc agcagcagca
     3181 gcaacagcag cagcagcagc agcagcagca gcagcaacaa cagctcaacc acgttgatgg
     3241 ttccagcaag cctgcggtgc tggcggcccc gtctggcctg gagcgctacg gcctaagcgc
     3301 tgccgccgcc gccgccgccg ccgccgccgc tgcggtggaa cagcgcagcc gcttcgagta
     3361 cccgccaccg ccggtgagcc tgggaagcag cagccacacc gcgcgactgc ccaacggcct
     3421 ggggggccca aacggcttcc ccaaaccaac accagaggag ggacccccag agctgaaccg
     3481 tcagagcccc aattcttctt cagcggcggc gtcggtggcg tctcggcgtg gaacgcacgg
     3541 tgggctggtt acggggctgc ccaacccggg gggtggcgga ggcccccagc tcaccgtgcc
     3601 ccccaacctg ctaccgcaga cgctgcttaa cggcccggcc agcgctgcgg tactcccccc
     3661 accccctccc cacgccctgg gcagccgtgg gcccccgacg cctgctcccc caggggctcc
     3721 tgggggcccc gcttgtctcg ggggtacccc gggtgtatcg gccacgtcgt cctccgcgtc
     3781 gtcttcgacc tcttcgtcgg tggcagaggt gggcgtgggt gctggtggta agaggcccgg
     3841 ctcggtgtcg agcacagacc aggagcgcga gttgaaggag aagcagcgca acgccgaggc
     3901 cctggccgag ctgagcgaga gcctgcgcaa ccgcgccgag gagtgggcca gcaagcccaa
     3961 gatggtccgc gacacgctgc tcacgctggc aggctgcacg ccctacgagg ttcgcttcaa
     4021 gaaggaccac tcgctgctgg gccgcgtttt cgccttcgac gccgtctcca agcccggcat
     4081 ggactacgaa ttgaagctgt tcattgagta ccccacgggc tcgggcaacg tgtactccag
     4141 tgcatctggt gtggccaagc agatgtatca ggactgcatg aaggacttcg gccggggcct
     4201 atcctcgggt ttcaagtacc tggagtacga aaagaagcac ggctccgggg actggcgcct
     4261 gcttggagac ctgctccccg aagccgtgcg cttcttcaag gagggcgtgc ccggcgccga
     4321 catgctgccc cagccctacc tggacgccag ctgtcccatg ctgcccactg ctctggtgag
     4381 tctgagccgc gcccccagcg cacccccggg gaccggggcc ttgccgcccg ccgcgccgtc
     4441 gggccggggc gcagccgcca gcctgcgcaa gagaaaggcc tctccggagc ccccggactc
     4501 agccgagggc gcgctgaagc tgggcgagga acagcagagg cagcagtgga tggcgaacca
     4561 gagcgaggcg ctgaagctca ccatgtccgc cgggggcttc gcggcgccgg ggcacgcggc
     4621 ggggggtccg cctccgccgc ccccacctct gggaccccat tccaaccgga ccaccccacc
     4681 tgagtcagcc ccccagaacg gtccgtcccc tatggccgct ctcatgtcgg tggcagatac
     4741 tctgggcaca gcgcactcgc ccaaggatgg cagttccgtg cactctacca ctgcgtcggc
     4801 gcggcgaaac agcagcagcc cagtctcgcc ggcctccgtg ccggggcagc gccgcttggc
     4861 atcacgtaac ggggacctga atttacaggt ggcgcccccg ccgcctagcg cccacccggg
     4921 catggaccaa gtgcaccccc aaaacattcc ggattccccc atggccaaca gcggacccct
     4981 ctgctgcacc atttgccacg aacgtttgga ggatacgcat ttcgttcagt gcccttccgt
     5041 ccccagccac aaattttgct tcccttgctc tagagagagt atcaaggccc agggggccac
     5101 cggcgaggtg tattgcccca gcggagagaa atgcccccta gtcgggtcga atgtaccttg
     5161 ggccttcatg cagggcgaaa tcgcgactat cttagctggg gatgttaaag tgaaaaagga
     5221 gagagaccct tgaggatccc gcgactctag ataattctac cgggtagggg aggcgctttt
     5281 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     5341 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     5401 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     5461 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     5521 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     5581 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     5641 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     5701 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     5761 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5821 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5881 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5941 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6001 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6061 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6121 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6181 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6241 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6301 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6361 acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg
     6421 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6481 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6541 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6601 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6661 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6721 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6781 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     6841 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6901 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6961 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     7021 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7081 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7141 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     7201 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7261 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     7321 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     7381 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     7441 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     7501 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     7561 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     7621 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7801 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7861 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt
     7921 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7981 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8041 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8101 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8161 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8761 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9121 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9181 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9241 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9301 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9361 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9421 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9481 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9541 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9601 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9661 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9721 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9781 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9841 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9901 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9961 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10021 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10081 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
    10141 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10201 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    10261 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    10321 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    10381 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt
    10441 gtatttgtta aatatgtact acaaacttag tagt
//
