LOCUS V038468 9286 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038468 VERSION V038468 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3982 /label="FOS(NM_005252)" /note="FOS(NM_005252)" /gene="FOS" CDS 3989..4054 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4074..4573 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4594..5193 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5207..5795 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6002..6635 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6764..6780) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6788..6804 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6812..6842) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6857..6878 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7166..7754) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7925..8785) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8786..8890) /gene="bla" /label="AmpR promoter" polyA_signal 8938..9072 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgatgtt ctcgggcttc aacgcagact acgaggcgtc 2881 atcctcccgc tgcagcagcg cgtccccggc cggggatagc ctctcttact accactcacc 2941 cgcagactcc ttctccagca tgggctcgcc tgtcaacgcg caggacttct gcacggacct 3001 ggccgtctcc agtgccaact tcattcccac ggtcactgcc atctcgacca gtccggacct 3061 gcagtggctg gtgcagcccg ccctcgtctc ctccgtggcc ccatcgcaga ccagagcccc 3121 tcaccctttc ggagtccccg ccccctccgc tggggcttac tccagggctg gcgttgtgaa 3181 gaccatgaca ggaggccgag cgcagagcat tggcaggagg ggcaaggtgg aacagttatc 3241 tccagaagaa gaagagaaaa ggagaatccg aagggaaagg aataagatgg ctgcagccaa 3301 atgccgcaac cggaggaggg agctgactga tacactccaa gcggagacag accaactaga 3361 agatgagaag tctgctttgc agaccgagat tgccaacctg ctgaaggaga aggaaaaact 3421 agagttcatc ctggcagctc accgacctgc ctgcaagatc cctgatgacc tgggcttccc 3481 agaagagatg tctgtggctt cccttgatct gactgggggc ctgccagagg ttgccacccc 3541 ggagtctgag gaggccttca ccctgcctct cctcaatgac cctgagccca agccctcagt 3601 ggaacctgtc aagagcatca gcagcatgga gctgaagacc gagccctttg atgacttcct 3661 gttcccagca tcatccaggc ccagtggctc tgagacagcc cgctccgtgc cagacatgga 3721 cctatctggg tccttctatg cagcagactg ggagcctctg cacagtggct ccctggggat 3781 ggggcccatg gccacagagc tggagcccct gtgcactccg gtggtcacct gtactcccag 3841 ctgcactgct tacacgtctt ccttcgtctt cacctacccc gaggctgact ccttccccag 3901 ctgtgcagct gcccaccgca agggcagcag cagcaatgag ccttcctctg actcgctcag 3961 ctcacccacg ctgctggccc tgggatccga ctacaaagac catgacggtg attataaaga 4021 tcatgacatc gactacaagg atgacgatga caagtagtct agataattct accgggtagg 4081 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 4141 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 4201 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 4261 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 4321 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 4381 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 4441 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 4501 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 4561 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 4621 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 4681 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 4741 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 4801 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 4861 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 4921 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 4981 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5041 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5101 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5161 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 5221 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5281 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5341 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5401 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5461 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5521 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5581 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5641 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 5701 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 5761 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 5821 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 5881 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 5941 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 6001 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 6061 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 6121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 6181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 6241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 6301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 6361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 6421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 6481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6601 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6661 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 6721 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 6781 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6841 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6901 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6961 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 7021 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 7081 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7141 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7201 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7261 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7321 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 7381 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 7441 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7501 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7561 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 7621 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7681 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 7741 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 7801 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7861 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7921 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7981 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 8041 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 8101 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8161 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8221 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 8281 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 8341 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 8401 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 8461 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 8521 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8581 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 8641 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 8701 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 8761 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 8821 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8881 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 8941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 9001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 9061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 9121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 9181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt 9241 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //