LOCUS       V043971                10624 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043971
VERSION     V043971
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5320
                     /label="ELAC2(NM_018127)"
                     /note="ELAC2(NM_018127)"
                     /gene="ELAC2"
     CDS             5327..5392
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5412..5911
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5932..6531
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6545..7133
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7340..7973
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8102..8118)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8126..8142
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8150..8180)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8195..8216
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8504..9092)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9263..10123)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10124..10228)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10276..10410
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgtgggc gctttgctcg ctgctgcggt ccgcggccgg
     2881 acgcaccatg tcgcagggac gcaccatatc gcaggcaccc gcccgccgcg agcggccgcg
     2941 caaggacccg ctgcggcacc tgcgcacgcg agagaagcgc ggaccgtcgg ggtgctccgg
     3001 cggcccaaac accgtgtacc tgcaggtggt ggcagcgggt agccgggact cgggcgccgc
     3061 gctctacgtc ttctccgagt tcaaccggta tctcttcaac tgtggagaag gcgttcagag
     3121 actcatgcag gagcacaagt taaaggttgc tcgcctggac aacatattcc tgacacgaat
     3181 gcactggtct aatgttgggg gcttaagtgg aatgattctt actttaaagg aaaccgggct
     3241 tccaaagtgt gtactttctg gacctccaca actggaaaaa tacctcgaag caatcaaaat
     3301 attttctggt ccattgaaag gaatagaact ggctgtgcgg ccccactctg ccccagaata
     3361 cgaggatgaa accatgacag tttaccagat ccccatacac agtgaacaga ggaggggaaa
     3421 gcaccaacca tggcagagtc cagaaaggcc tctcagcagg ctcagtccag agcgatcttc
     3481 agactccgag tcgaatgaaa atgagccaca ccttccacat ggtgttagcc agagaagagg
     3541 ggtcagggac tcttccctgg tcgtagcttt catctgtaag cttcacttaa agagaggaaa
     3601 cttcttggtg ctcaaagcaa aggagatggg cctcccagtt gggacagctg ccatcgctcc
     3661 catcattgct gctgtcaagg acgggaaaag catcactcat gaaggaagag agattttggc
     3721 tgaagagctg tgtactcctc cagatcctgg tgctgctttt gtggtggtag aatgtccaga
     3781 tgaaagcttc attcaaccca tctgtgagaa tgccaccttt cagaggtacc aaggaaaggc
     3841 agatgccccc gtggccttgg tggttcacat ggccccagca tctgtgcttg tggacagcag
     3901 gtaccagcag tggatggaga ggtttgggcc tgacacccag cacttggtcc tgaatgagaa
     3961 ctgtgcctca gttcacaacc ttcgcagcca caagattcaa acccagctca acctcatcca
     4021 cccggacatc ttccccctgc tcaccagttt ccgctgtaag aaggagggcc ccaccctcag
     4081 tgtgcccatg gttcagggtg aatgcctcct caagtaccag ctccgtccca ggagggagtg
     4141 gcagagggat gccattatta cttgcaatcc tgaggaattc atagttgagg cgctgcagct
     4201 tcccaacttc cagcagagcg tgcaggagta caggaggagt gcgcaggacg gcccagcccc
     4261 agcagagaaa agaagtcagt acccagaaat catcttcctt ggaacagggt ctgccatccc
     4321 gatgaagatt cgaaatgtca gtgccacact tgtcaacata agccccgaca cgtctctgct
     4381 actggactgt ggtgagggca catttgggca gctgtgccgt cattacggag accaggtgga
     4441 cagggtcctg ggcaccctgg ctgctgtgtt tgtgtcccac ctgcacgcag atcaccacac
     4501 gggcttgcca agtatcttgc tgcagagaga acgcgccttg gcatctttgg gaaagccgct
     4561 tcaccctttg ctggtggttg cccccaacca gctcaaagcc tggctccagc agtaccacaa
     4621 ccagtgccag gaggtcctgc accacatcag tatgattcct gccaaatgcc ttcaggaagg
     4681 ggctgagatc tccagtcctg cagtggaaag attgatcagt tcgctgttgc gaacatgtga
     4741 tttggaagag tttcagacct gtctggtgcg gcactgcaag catgcgtttg gctgtgcgct
     4801 ggtgcacacc tctggctgga aagtggtcta ttccggggac accatgccct gcgaggctct
     4861 ggtccggatg gggaaagatg ccaccctcct gatacatgaa gccaccctgg aagatggttt
     4921 ggaagaggaa gcagtggaaa agacacacag cacaacgtcc caagccatca gcgtggggat
     4981 gcggatgaac gcggagttca ttatgctgaa ccacttcagc cagcgctatg ccaaggtccc
     5041 cctcttcagc cccaacttca gcgagaaagt gggagttgcc tttgaccaca tgaaggtctg
     5101 ctttggagac tttccaacaa tgcccaagct gattccccca ctgaaagccc tgtttgctgg
     5161 cgacatcgag gagatggagg agcgcaggga gaagcgggag ctgcggcagg tgcgggcggc
     5221 cctcctgtcc agggagctgg caggcggcct ggaggatggg gagcctcagc agaagcgggc
     5281 ccacacagag gagccacagg ccaagaaggt cagagcccag ggatccgact acaaagacca
     5341 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaatctag
     5401 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     5461 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     5521 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     5581 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     5641 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     5701 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     5761 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     5821 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     5881 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     5941 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6001 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6061 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6121 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6181 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6241 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6301 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6361 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6421 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6481 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg
     6541 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6601 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6661 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6721 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6781 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6841 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6901 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     6961 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7021 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7081 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     7141 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7201 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7261 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7321 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7381 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     7441 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     7501 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     7561 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     7621 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     7681 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     7741 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     7801 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7861 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7921 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7981 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8041 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat
     8101 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8161 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8221 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8281 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8341 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8401 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8461 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8521 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8581 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8641 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8701 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8761 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8821 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8881 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8941 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9001 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9061 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9121 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9181 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9241 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9301 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9361 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9421 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9481 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9541 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9601 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9661 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9721 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9781 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9841 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9901 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9961 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10021 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10081 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10201 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10261 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10321 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10381 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    10441 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    10501 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    10561 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10621 tagt
//
