LOCUS       V031423                11394 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031423
VERSION     V031423
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..3543
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3550..6150
                     /label="E2F8(NM_024680)"
                     /note="E2F8(NM_024680)"
                     /gene="E2F8"
     promoter        6182..6681
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6702..7301
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7315..7903
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8110..8743
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8872..8888)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8896..8912
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8920..8950)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8965..8986
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9274..9862)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10033..10893)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10894..10998)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11046..11180
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2881 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2941 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3001 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3061 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3121 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3181 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3241 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3301 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3361 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3421 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3481 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3541 aaggaattca tggagaacga aaaggaaaat ctcttttgtg agccacataa aaggggacta
     3601 atgaaaacac ctctgaaaga atccaccaca gcaaatatcg tgttggcaga gatccagcct
     3661 gactttggcc ctttaaccac acctaccaag cccaaggaag gctctcaggg agagccgtgg
     3721 acaccgacag ccaacctgaa aatgctcatc agtgctgtga gccctgagat ccgcaacaga
     3781 gatcagaaaa ggggtttgtt tgacaacaga agtggattac ctgaggccaa agactgtata
     3841 cacgaacact tatctggaga tgaatttgag aaatcccaac caagtcgaaa agagaaaagt
     3901 ttaggattat tgtgtcataa gttcttagca cgatatccta attatcccaa ccctgctgtg
     3961 aataatgaca tctgccttga cgaagtggca gaggaactta atgttgaacg tcgacgcatt
     4021 tacgatatcg tgaacgtcct agagagttta catatggtga gccgcctcgc caaaaacagg
     4081 tacacttggc acgggcgaca caatctcaac aaaacccttg gcaccttgaa gagcatcggg
     4141 gaggagaata agtacgccga gcagattatg atgatcaaaa agaaagaata tgagcaagag
     4201 tttgacttta ttaagagtta cagtatagag gatcatatca tcaaatcaaa cactggccca
     4261 aatggacacc cagacatgtg ttttgtggaa ctccctggag tggaatttcg ggcagcttct
     4321 gtaaacagcc gcaaagacaa gtctttaagg gtaatgagcc agaaatttgt gatgctgttt
     4381 ttggtgtcaa cgcctcagat agtaagccta gaagttgctg ccaagatttt aattggggag
     4441 gaccatgtgg aagatttgga taaaagcaag tttaaaacaa aaattaggag gttgtatgat
     4501 atagctaatg ttctgagtag cctggatctt atcaagaaag ttcatgttac agaggaaaga
     4561 ggccgaaaac cagctttcaa atggaccggc ccagaaatca gtccaaatac cagtggctcc
     4621 agcccagtca ttcattttac tccctctgat ttggaggtga gacggtcttc aaaagagaac
     4681 tgtgccaaaa acctcttttc cacacgtggg aaaccaaact ttactcgaca cccatctctt
     4741 atcaaattgg taaagagtat agaaagtgat cggagaaaga taaattctgc gcccagtagc
     4801 cctatcaaga ccaacaaagc tgagagttct cagaattctg cacccttccc aagtaaaatg
     4861 gctcagctcg cagctatttg taaaatgcag ttagaagagc aatcaagtga atccagacag
     4921 aaagtgaaag tacagctggc aagatctgga ccctgcaaac cagtagcccc tctggacccc
     4981 ccagtgaatg ctgagatgga gctgacagca ccgtccctca tccagcccct gggaatggtt
     5041 cccctgatcc ccagcccctt gtcatcagca gtgcccctga tcctacctca ggccccttca
     5101 ggcccatcct atgccatcta cctgcagccc actcaagccc accaaagtgt gacgccaccc
     5161 caaggcctga gcccaacggt gtgcaccacc cactcttcta aagctactgg ctcaaaagac
     5221 tccacagatg ccaccactga gaaggcagcc aatgatacct caaaggccag tgcctctacc
     5281 aggcctggaa gcttgctgcc agcaccagag aggcaagggg caaagagccg aaccagggag
     5341 ccagctggag aaagaggctc aaagagggca agcatgctcg aggacagtgg ttccaaaaag
     5401 aaatttaaag aggacctaaa aggacttgaa aatgtctccg caaccttgtt cccatcagga
     5461 tacctaatcc ctctcacgca gtgctcatcc ctgggggcag agtccatttt gtctggtaaa
     5521 gaaaactcaa gtgctctttc cccaaaccac aggatttaca gctccccaat tgcaggtgtt
     5581 attccagtga catcatctga actcactgct gttaattttc cctcttttca tgtaacaccg
     5641 ttgaagctaa tggtctcacc aacttccgtg gcagccgtac ctgtcgggaa cagcccggct
     5701 ctcgcttcaa gccaccctgt tcccatccag aacccaagct cagccattgt aaacttcacc
     5761 ctgcagcacc tgggactcat ctcacccaat gtgcagttgt ctgccagccc tgggtctgga
     5821 atcgttcctg tgtctccaag aatagagtct gttaatgtcg caccagaaaa tgcaggcact
     5881 cagcaaggaa gggccaccaa ctatgactca ccagtcccag gccagagcca gccaaatgga
     5941 caatcagttg ctgtgacagg ggcacaacag cctgttcctg tgacacccaa agggtcacaa
     6001 ttagtggccg aaagtttctt ccgtacccca ggtggaccca ccaagccaac cagctcatcc
     6061 tgcatggatt ttgagggtgc taataaaacc tccttaggaa ctctctttgt cccacagcga
     6121 aaactggaag tctcaacaga ggatgtccat taaggatccc gcgactctag ataattctac
     6181 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     6241 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     6301 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     6361 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     6421 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     6481 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     6541 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     6601 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     6661 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     6721 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6781 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6841 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6901 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6961 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     7021 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     7081 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     7141 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7201 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     7261 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa
     7321 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7381 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7441 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7501 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7561 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7621 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7681 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     7741 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7801 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7861 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
     7921 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     7981 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     8041 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8101 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     8161 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     8221 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     8281 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     8341 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     8401 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     8461 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     8521 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     8581 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8641 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8701 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     8761 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     8821 cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     8881 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8941 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9001 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9061 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10021 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10081 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10141 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10201 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10261 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10321 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10381 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10441 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10501 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10561 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10621 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10681 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10741 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10801 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10861 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10981 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
    11041 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    11101 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    11161 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
    11221 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
    11281 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    11341 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
