LOCUS V039935 10552 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039935 VERSION V039935 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5227 /label="DHX15(NM_001358)" /note="DHX15(NM_001358)" /gene="DHX15" CDS 5234..5260 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5264..5290 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5294..5320 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5340..5839 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5860..6459 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6473..7061 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7268..7901 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8030..8046) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8054..8070 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8078..8108) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8123..8144 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8432..9020) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9191..10051) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10052..10156) /gene="bla" /label="AmpR promoter" polyA_signal 10204..10338 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgtccaa gcggcaccgg ttggacctag gggaggatta 2881 cccctctggc aagaagcgtg cggggaccga tgggaaggat cgagatcgag accgggatcg 2941 tgaagatcgg tctaaagatc gagaccgaga acgtgataga ggagatagag agcgagagag 3001 ggagaaagaa aaggagaagg agttgcgagc ttcaacaaat gctatgctta tcagtgctgg 3061 attaccacct ttgaaagctt cccattcagc tcactcaacc cactcagcac attcaacgca 3121 ttcaacacat tctgctcatt caacgcatgc cggacatgca ggtcacacgt cacttccaca 3181 gtgcattaat ccgttcacca acttacccca tactcctcga tactatgata ttctaaagaa 3241 acgtcttcag ctccctgttt gggaatacaa ggataggttt acagatattc tggttagaca 3301 tcagtccttt gtactggttg gtgagactgg gtctggtaaa acaacacaga ttccacagtg 3361 gtgtgtggag tacatgcgat cattaccagg acccaagaga ggagttgcct gtacccaacc 3421 caggagagtg gctgcaatga gtgtggctca gagagttgct gatgagatgg atgtgatgtt 3481 gggccaggaa gttggttact ccattcgatt tgaagactgc agtagtgcaa aaaccattct 3541 taagtatatg actgatggga tgttacttcg tgaagctatg aatgatcccc tcctggagcg 3601 ttatggtgta ataattcttg atgaggctca tgagaggaca ctggctacag atattctaat 3661 gggtgttctg aaggaagttg taagacagag atcagattta aaggttatag ttatgagcgc 3721 tactctagat gcaggaaaat tccagattta ctttgataac tgtcctctcc taactattcc 3781 tgggcgtaca catcctgttg agatcttcta tactccagaa ccagagagag attatcttga 3841 agcagcaatt cgaacagtta tccagattca tatgtgtgaa gaggaagagg gagatcttct 3901 tcttttctta actggtcaag aggaaattga tgaagcctgt aagagaataa agcgtgaagt 3961 tgatgatttg ggccctgaag ttggtgacat taaaatcatt ccattgtatt ctacacttcc 4021 acctcagcag cagcaacgca tttttgagcc tccacctccc aaaaaacaga atggagcaat 4081 tggaagaaag gtagttgtgt caactaacat agcagagacg tctttgacaa tagatggtgt 4141 ggtgtttgtg attgatcctg gatttgcgaa acagaaggtc tacaatcctc gaatcagagt 4201 tgagtccctt ttggtgacag ctattagtaa agcttcagct cagcaaaggg ctggtcgagc 4261 tggacgtacc agacctggaa aatgcttcag actttacaca gagaaagctt ataaaacaga 4321 aatgcaggat aacacctatc ctgagatttt gcgttctaat ttaggatcag ttgtgttaca 4381 attgaagaaa cttggtattg atgacttggt acattttgat tttatggatc caccagctcc 4441 tgaaactctg atgagagccc tggaactttt gaattacctg gctgctttaa atgatgatgg 4501 agatctgact gaattgggat ccatgatggc agagtttcct ctagatccac agctcgcaaa 4561 aatggttatt gcaagttgtg actacaactg ttctaatgag gtcctatcta ttactgctat 4621 gttgtcagtc ccacagtgtt ttgttcgccc cacggaggcc aagaaagccg cagatgaggc 4681 caagatgaga tttgcccaca tagatggaga tcatctgaca ctgctgaacg tctaccatgc 4741 ttttaaacaa aatcatgaat cggttcagtg gtgttatgac aacttcatta actacaggtc 4801 cctgatgtcc gcagacaatg tacgccagca gctatctcga attatggaca gatttaattt 4861 gcctcgtcga agtactgact ttacaagcag ggactattat attaatataa gaaaagcttt 4921 ggttactggg tattttatgc aggtggcaca tttagaacga acagggcatt acttaactgt 4981 gaaagataac caggtggttc agttgcatcc ctctactgtt cttgaccaca aacctgaatg 5041 ggtgctttat aatgagtttg ttctaacaac aaagaattac atccggacat gtacagatat 5101 caagccagaa tggttggtga aaattgcccc tcaatattat gacatgagca atttcccaca 5161 gtgtgaagca aagagacagt tggaccgcat cattgccaaa cttcaatcca aggaatattc 5221 acagtacgga tcctacccat acgacgtacc tgattatgca acttatcctt atgatgtacc 5281 tgattatgct agctatcctt atgatgtccc agactacgca tagtctagat aattctaccg 5341 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 5401 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 5461 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 5521 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 5581 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 5641 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 5701 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 5761 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 5821 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 5881 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 5941 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 6001 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 6061 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 6121 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 6181 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 6241 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 6301 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 6361 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 6421 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 6481 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 6541 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 6601 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 6661 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 6721 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 6781 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 6841 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 6901 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 6961 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 7021 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 7081 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 7141 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 7201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 7261 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 7321 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 7381 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 7441 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 7501 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 7561 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 7621 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 7681 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 7741 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 7801 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 7861 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 7921 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 7981 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 8041 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 8101 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 8161 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 8221 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 8281 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 8341 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 8401 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 8461 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 8521 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 8581 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 8641 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 8701 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 8761 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 8821 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 8881 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 8941 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 9001 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 9061 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 9121 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 9181 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 9241 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 9301 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 9361 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 9421 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 9481 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 9541 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 9601 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 9661 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 9721 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 9781 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 9841 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 9901 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 9961 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 10021 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 10081 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 10141 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 10201 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 10261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 10321 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 10381 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 10441 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 10501 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt //