LOCUS       V039935                10552 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039935
VERSION     V039935
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5227
                     /label="DHX15(NM_001358)"
                     /note="DHX15(NM_001358)"
                     /gene="DHX15"
     CDS             5234..5260
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5264..5290
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5294..5320
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5340..5839
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5860..6459
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6473..7061
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7268..7901
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8030..8046)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8054..8070
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8078..8108)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8123..8144
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8432..9020)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9191..10051)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10052..10156)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10204..10338
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgtccaa gcggcaccgg ttggacctag gggaggatta
     2881 cccctctggc aagaagcgtg cggggaccga tgggaaggat cgagatcgag accgggatcg
     2941 tgaagatcgg tctaaagatc gagaccgaga acgtgataga ggagatagag agcgagagag
     3001 ggagaaagaa aaggagaagg agttgcgagc ttcaacaaat gctatgctta tcagtgctgg
     3061 attaccacct ttgaaagctt cccattcagc tcactcaacc cactcagcac attcaacgca
     3121 ttcaacacat tctgctcatt caacgcatgc cggacatgca ggtcacacgt cacttccaca
     3181 gtgcattaat ccgttcacca acttacccca tactcctcga tactatgata ttctaaagaa
     3241 acgtcttcag ctccctgttt gggaatacaa ggataggttt acagatattc tggttagaca
     3301 tcagtccttt gtactggttg gtgagactgg gtctggtaaa acaacacaga ttccacagtg
     3361 gtgtgtggag tacatgcgat cattaccagg acccaagaga ggagttgcct gtacccaacc
     3421 caggagagtg gctgcaatga gtgtggctca gagagttgct gatgagatgg atgtgatgtt
     3481 gggccaggaa gttggttact ccattcgatt tgaagactgc agtagtgcaa aaaccattct
     3541 taagtatatg actgatggga tgttacttcg tgaagctatg aatgatcccc tcctggagcg
     3601 ttatggtgta ataattcttg atgaggctca tgagaggaca ctggctacag atattctaat
     3661 gggtgttctg aaggaagttg taagacagag atcagattta aaggttatag ttatgagcgc
     3721 tactctagat gcaggaaaat tccagattta ctttgataac tgtcctctcc taactattcc
     3781 tgggcgtaca catcctgttg agatcttcta tactccagaa ccagagagag attatcttga
     3841 agcagcaatt cgaacagtta tccagattca tatgtgtgaa gaggaagagg gagatcttct
     3901 tcttttctta actggtcaag aggaaattga tgaagcctgt aagagaataa agcgtgaagt
     3961 tgatgatttg ggccctgaag ttggtgacat taaaatcatt ccattgtatt ctacacttcc
     4021 acctcagcag cagcaacgca tttttgagcc tccacctccc aaaaaacaga atggagcaat
     4081 tggaagaaag gtagttgtgt caactaacat agcagagacg tctttgacaa tagatggtgt
     4141 ggtgtttgtg attgatcctg gatttgcgaa acagaaggtc tacaatcctc gaatcagagt
     4201 tgagtccctt ttggtgacag ctattagtaa agcttcagct cagcaaaggg ctggtcgagc
     4261 tggacgtacc agacctggaa aatgcttcag actttacaca gagaaagctt ataaaacaga
     4321 aatgcaggat aacacctatc ctgagatttt gcgttctaat ttaggatcag ttgtgttaca
     4381 attgaagaaa cttggtattg atgacttggt acattttgat tttatggatc caccagctcc
     4441 tgaaactctg atgagagccc tggaactttt gaattacctg gctgctttaa atgatgatgg
     4501 agatctgact gaattgggat ccatgatggc agagtttcct ctagatccac agctcgcaaa
     4561 aatggttatt gcaagttgtg actacaactg ttctaatgag gtcctatcta ttactgctat
     4621 gttgtcagtc ccacagtgtt ttgttcgccc cacggaggcc aagaaagccg cagatgaggc
     4681 caagatgaga tttgcccaca tagatggaga tcatctgaca ctgctgaacg tctaccatgc
     4741 ttttaaacaa aatcatgaat cggttcagtg gtgttatgac aacttcatta actacaggtc
     4801 cctgatgtcc gcagacaatg tacgccagca gctatctcga attatggaca gatttaattt
     4861 gcctcgtcga agtactgact ttacaagcag ggactattat attaatataa gaaaagcttt
     4921 ggttactggg tattttatgc aggtggcaca tttagaacga acagggcatt acttaactgt
     4981 gaaagataac caggtggttc agttgcatcc ctctactgtt cttgaccaca aacctgaatg
     5041 ggtgctttat aatgagtttg ttctaacaac aaagaattac atccggacat gtacagatat
     5101 caagccagaa tggttggtga aaattgcccc tcaatattat gacatgagca atttcccaca
     5161 gtgtgaagca aagagacagt tggaccgcat cattgccaaa cttcaatcca aggaatattc
     5221 acagtacgga tcctacccat acgacgtacc tgattatgca acttatcctt atgatgtacc
     5281 tgattatgct agctatcctt atgatgtccc agactacgca tagtctagat aattctaccg
     5341 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5401 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5461 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5521 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5581 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5641 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5701 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5761 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5821 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     5881 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5941 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6001 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6061 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6121 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6181 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6241 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6301 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6361 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6421 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc
     6481 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6541 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6601 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6661 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6721 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6781 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6841 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6901 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6961 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7021 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     7081 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     7141 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     7201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7261 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     7321 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     7381 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     7441 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     7501 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     7561 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     7621 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     7681 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     7741 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7801 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7861 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7921 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     7981 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8041 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8101 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8161 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8221 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8281 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8341 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8401 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8461 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8521 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8581 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8641 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8701 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8761 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8821 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8881 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8941 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9001 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9061 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9121 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9181 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9241 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9301 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9361 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9421 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9481 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9541 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9601 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9661 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9721 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9781 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9841 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9901 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9961 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10021 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10081 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10141 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10201 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10321 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    10381 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    10441 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10501 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
