LOCUS V034594 10261 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V034594 VERSION V034594 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..4936 /label="CPEB3(NM_014912)" /note="CPEB3(NM_014912)" /gene="CPEB3" CDS 4943..4969 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4973..4999 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5003..5029 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5049..5548 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5569..6168 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6182..6770 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6977..7610 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7739..7755) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7763..7779 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7787..7817) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7832..7853 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8141..8729) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8900..9760) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9761..9865) /gene="bla" /label="AmpR promoter" polyA_signal 9913..10047 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgcagga tgatttactg atggacaaaa gcaaaaccca 2881 gccccagccc cagcagcagc agcggcagca gcagcagccc caacctgagt ccagcgtatc 2941 cgaagccccg tccacgcccc tctcctcaga gacccccaag ccggaggaaa acagcgcagt 3001 gccggccctc agcccagccg ctgccccccc ggcccccaac ggcccggaca agatgcagat 3061 ggaatcaccg ctcctgccag gcttgagttt ccatcagcct cctcagcagc cgccgccgcc 3121 tcaggagccc gcggcaccgg gcgcgtcgct gtcgccgtcc ttcggcagca cctggtccac 3181 gggcaccacc aacgcggtag aggacagctt cttccagggg atcaccccag tcaacgggac 3241 catgctcttc cagaacttcc cgcaccatgt caacccagtc ttcggaggca ctttctcccc 3301 gcagatcggc ctggcgcaga cccagcacca ccagcagccg ccgccgcctg cgcccgcgcc 3361 gcagccggca cagccagcgc agccaccaca ggcgcagccc ccgcagcagc gccgctcacc 3421 cgccagcccc agccaggcgc cctacgcgca gaggagcgcc gccgcggcgt acggccacca 3481 gcccatcatg accagcaagc cgtcctcgtc ttcggcggtt gcagccgctg ctgccgcagc 3541 cgccgcctcg tcggcctcgt ccagctggaa cacgcaccaa agcgtgaatg cagcctggag 3601 cgcaccgtcc aacccctggg gcggcctgca ggcgggccgg gaccctcgcc gggcggtcgg 3661 tgtgggcgtg ggtgtgggtg tcggggtgcc ttccccgctc aaccccatct cgccgctcaa 3721 aaagcccttc tccagcaacg tgatcgcgcc gcccaagttc cctcgcgcgg cccctctcac 3781 ttccaagtcc tggatggagg ataacgcttt ccggaccgat aatggtaaca atctgttgcc 3841 atttcaggac cggagtaggc cctatgatac ttttaacttg cactcgttgg agaactcctt 3901 aatggatatg ataaggactg atcatgaacc tctgaaaggt aaacactacc ctcccagtgg 3961 cccaccaatg agtttcgctg atataatgtg gaggaatcat tttgcaggac gcatggggat 4021 aaatttccat catccaggaa cagataatat tatggcactt aacaatgcct tcctggatga 4081 tagccatggt gatcaagcct tgtcatctgg cttaagttct cccactcgct gtcaaaatgg 4141 ggaacgagta gaacgctact ctagaaaggt gtttgttgga ggacttcctc ctgatattga 4201 tgaagatgag atcactgcca gctttcgcag gtttggacct ctcgtagtag actggcctca 4261 caaagctgaa agcaagtctt attttcctcc taaaggctat gcctttctgc tgttccaaga 4321 ggaaagctca gtacaagctt tgatagatgc ctgcctagaa gaagatggga aactctacct 4381 gtgtgtgtca agccccacca tcaaggacaa gccagtgcaa attcgaccat ggaacctaag 4441 tgacagtgac tttgtaatgg atggttctca gcctttggac cccagaaaaa ctatctttgt 4501 tgggggagtt ccacgacccc ttcgagctgt tgaactggca atgatcatgg accgtttgta 4561 cggtggtgtc tgctatgctg gcattgatac ggacccagag ctgaagtacc ccaaaggtgc 4621 tggccgcgtg gcattctcca atcagcagag ttacattgca gccatcagcg ctcgttttgt 4681 gcagcttcag cacaatgaca ttgacaaacg ggttgaagta aagccatatg tgctggatga 4741 tcagatgtgt gatgagtgcc agggcacacg ctgtggtggg aagtttgccc cgttcttctg 4801 tgccaacgtc acctgtctgc agtattactg tgaatactgc tgggcgagca tacattcccg 4861 agccgggcgg gagttccaca aaccgctggt gaaggaggga ggcgaccgcc ctcgtcacgt 4921 cccgttccgc tggagcggat cctacccata cgacgtacct gattatgcaa cttatcctta 4981 tgatgtacct gattatgcta gctatcctta tgatgtccca gactacgcat agtctagata 5041 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 5101 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 5161 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 5221 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 5281 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 5341 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 5401 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 5461 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 5521 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 5581 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5641 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 5701 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 5761 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5821 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5881 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5941 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6001 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 6061 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 6121 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 6181 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 6241 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 6301 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6361 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6421 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6481 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6541 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 6601 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6661 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6721 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 6781 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 6841 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 6901 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6961 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 7021 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 7081 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 7141 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 7201 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 7261 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 7321 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 7381 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 7441 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 7501 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 7561 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 7621 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 7681 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 7741 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7801 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7861 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7921 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 8041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 8101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 8161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 8221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 8281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 8341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 8401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 8461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 8521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 8581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 8701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 9001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 9061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 9121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 9181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 9241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 9301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 9361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 9481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 9541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 9901 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 9961 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 10021 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 10081 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 10141 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 10201 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 10261 t //