LOCUS       V034594                10261 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034594
VERSION     V034594
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4936
                     /label="CPEB3(NM_014912)"
                     /note="CPEB3(NM_014912)"
                     /gene="CPEB3"
     CDS             4943..4969
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4973..4999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5003..5029
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5049..5548
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5569..6168
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6182..6770
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6977..7610
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7739..7755)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7763..7779
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7787..7817)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7832..7853
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8141..8729)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8900..9760)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9761..9865)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9913..10047
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgcagga tgatttactg atggacaaaa gcaaaaccca
     2881 gccccagccc cagcagcagc agcggcagca gcagcagccc caacctgagt ccagcgtatc
     2941 cgaagccccg tccacgcccc tctcctcaga gacccccaag ccggaggaaa acagcgcagt
     3001 gccggccctc agcccagccg ctgccccccc ggcccccaac ggcccggaca agatgcagat
     3061 ggaatcaccg ctcctgccag gcttgagttt ccatcagcct cctcagcagc cgccgccgcc
     3121 tcaggagccc gcggcaccgg gcgcgtcgct gtcgccgtcc ttcggcagca cctggtccac
     3181 gggcaccacc aacgcggtag aggacagctt cttccagggg atcaccccag tcaacgggac
     3241 catgctcttc cagaacttcc cgcaccatgt caacccagtc ttcggaggca ctttctcccc
     3301 gcagatcggc ctggcgcaga cccagcacca ccagcagccg ccgccgcctg cgcccgcgcc
     3361 gcagccggca cagccagcgc agccaccaca ggcgcagccc ccgcagcagc gccgctcacc
     3421 cgccagcccc agccaggcgc cctacgcgca gaggagcgcc gccgcggcgt acggccacca
     3481 gcccatcatg accagcaagc cgtcctcgtc ttcggcggtt gcagccgctg ctgccgcagc
     3541 cgccgcctcg tcggcctcgt ccagctggaa cacgcaccaa agcgtgaatg cagcctggag
     3601 cgcaccgtcc aacccctggg gcggcctgca ggcgggccgg gaccctcgcc gggcggtcgg
     3661 tgtgggcgtg ggtgtgggtg tcggggtgcc ttccccgctc aaccccatct cgccgctcaa
     3721 aaagcccttc tccagcaacg tgatcgcgcc gcccaagttc cctcgcgcgg cccctctcac
     3781 ttccaagtcc tggatggagg ataacgcttt ccggaccgat aatggtaaca atctgttgcc
     3841 atttcaggac cggagtaggc cctatgatac ttttaacttg cactcgttgg agaactcctt
     3901 aatggatatg ataaggactg atcatgaacc tctgaaaggt aaacactacc ctcccagtgg
     3961 cccaccaatg agtttcgctg atataatgtg gaggaatcat tttgcaggac gcatggggat
     4021 aaatttccat catccaggaa cagataatat tatggcactt aacaatgcct tcctggatga
     4081 tagccatggt gatcaagcct tgtcatctgg cttaagttct cccactcgct gtcaaaatgg
     4141 ggaacgagta gaacgctact ctagaaaggt gtttgttgga ggacttcctc ctgatattga
     4201 tgaagatgag atcactgcca gctttcgcag gtttggacct ctcgtagtag actggcctca
     4261 caaagctgaa agcaagtctt attttcctcc taaaggctat gcctttctgc tgttccaaga
     4321 ggaaagctca gtacaagctt tgatagatgc ctgcctagaa gaagatggga aactctacct
     4381 gtgtgtgtca agccccacca tcaaggacaa gccagtgcaa attcgaccat ggaacctaag
     4441 tgacagtgac tttgtaatgg atggttctca gcctttggac cccagaaaaa ctatctttgt
     4501 tgggggagtt ccacgacccc ttcgagctgt tgaactggca atgatcatgg accgtttgta
     4561 cggtggtgtc tgctatgctg gcattgatac ggacccagag ctgaagtacc ccaaaggtgc
     4621 tggccgcgtg gcattctcca atcagcagag ttacattgca gccatcagcg ctcgttttgt
     4681 gcagcttcag cacaatgaca ttgacaaacg ggttgaagta aagccatatg tgctggatga
     4741 tcagatgtgt gatgagtgcc agggcacacg ctgtggtggg aagtttgccc cgttcttctg
     4801 tgccaacgtc acctgtctgc agtattactg tgaatactgc tgggcgagca tacattcccg
     4861 agccgggcgg gagttccaca aaccgctggt gaaggaggga ggcgaccgcc ctcgtcacgt
     4921 cccgttccgc tggagcggat cctacccata cgacgtacct gattatgcaa cttatcctta
     4981 tgatgtacct gattatgcta gctatcctta tgatgtccca gactacgcat agtctagata
     5041 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     5101 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     5161 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     5221 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     5281 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     5341 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     5401 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     5461 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     5521 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac
     5581 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5641 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5701 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5761 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5821 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5881 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5941 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6001 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6061 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6121 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa
     6181 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6241 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6301 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6361 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6421 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6481 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6541 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6601 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6661 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6721 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     6781 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     6841 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     6901 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6961 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     7021 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     7081 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     7141 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     7201 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     7261 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     7321 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     7381 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     7441 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7501 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7561 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7621 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7681 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt
     7741 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7801 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7861 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7921 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9901 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9961 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10021 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10081 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10141 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10201 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10261 t
//
