LOCUS V018525 8934 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018525 VERSION V018525 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2827..3660 /label="C1QTNF6(NM_031910)" /note="C1QTNF6(NM_031910)" /gene="C1QTNF6" CDS 3667..3690 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" promoter 3722..4221 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4242..4841 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4855..5443 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5650..6283 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6412..6428) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6436..6452 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6460..6490) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6505..6526 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6814..7402) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7573..8433) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8434..8538) /gene="bla" /label="AmpR promoter" polyA_signal 8586..8720 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgc agtggctcag ggtccgtgag tcgcctgggg aggccacagg acacagggtc 2881 accatgggga cagccgccct gggtcccgtc tgggcagcgc tcctgctctt tctcctgatg 2941 tgtgagatcc ctatggtgga gctcaccttt gacagagctg tggccagcgg ctgccaacgg 3001 tgctgtgact ctgaggaccc cctggatcct gcccatgtat cctcagcctc ttcctccggc 3061 cgcccccacg ccctgcctga gatcagaccc tacattaata tcaccatcct gaagggtgac 3121 aaaggggacc caggcccaat gggcctgcca gggtacatgg gcagggaggg tccccaaggg 3181 gagcctggcc ctcagggcag caagggtgac aagggggaga tgggcagccc cggcgccccg 3241 tgccagaagc gcttcttcgc cttctcagtg ggccgcaaga cggccctgca cagcggcgag 3301 gacttccaga cgctgctctt cgaaagggtc tttgtgaacc ttgatgggtg ctttgacatg 3361 gcgaccggcc agtttgctgc tcccctgcgt ggcatctact tcttcagcct caatgtgcac 3421 agctggaatt acaaggagac gtacgtgcac attatgcata accagaaaga ggctgtcatc 3481 ctgtacgcgc agcccagcga gcgcagcatc atgcagagcc agagtgtgat gctggacctg 3541 gcctacgggg accgcgtctg ggtgcggctc ttcaagcgcc agcgcgagaa cgccatctac 3601 agcaacgact tcgacaccta catcaccttc agcggccacc tcatcaaggc cgaggacgac 3661 gaattcgatt acaaggatga cgacgataag tgaggatccc gcgactctag ataattctac 3721 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg 3781 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg 3841 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc 3901 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca 3961 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt 4021 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg 4081 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc 4141 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct 4201 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca 4261 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt 4321 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca 4381 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg 4441 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg 4501 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc 4561 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca 4621 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg 4681 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca 4741 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag 4801 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa 4861 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 4921 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 4981 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5041 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 5101 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 5161 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 5221 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 5281 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 5341 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 5401 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca 5461 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg 5521 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg 5581 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5641 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga 5701 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca 5761 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag 5821 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg 5881 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg 5941 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg 6001 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag 6061 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac 6121 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 6181 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 6241 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 6301 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga 6361 cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 6421 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6481 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6541 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6601 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 7261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7441 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7501 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7561 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7621 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7681 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7741 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 7801 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 7861 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 7921 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 7981 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 8041 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 8101 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 8161 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8221 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 8281 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 8341 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8401 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 8461 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8521 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg 8581 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8641 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8701 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 8761 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg 8821 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact 8881 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //