LOCUS V024294 10354 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024294 VERSION V024294 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5050 /label="BACH1(NM_206866)" /note="BACH1(NM_206866)" /gene="BACH1" CDS 5057..5122 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5142..5641 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5662..6261 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6275..6863 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7070..7703 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7832..7848) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7856..7872 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7880..7910) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7925..7946 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8234..8822) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8993..9853) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9854..9958) /gene="bla" /label="AmpR promoter" polyA_signal 10006..10140 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgtctct gagtgagaac tcggtttttg cctatgaatc 2881 ttctgtgcat agcaccaatg ttttactcag ccttaatgac cagcggaaga aagatgtgct 2941 gtgcgatgtc accatctttg tggagggaca gcggttccgc gctcaccggt ccgtgctggc 3001 ggcatgcagc agttacttcc actcaagaat cgtaggccag gctgatggag agctgaacat 3061 tactcttcca gaagaggtga cagttaaagg atttgaacct ttaattcagt ttgcctacac 3121 tgctaaactg attttaagta aagagaatgt ggatgaagtg tgcaaatgtg tggagttttt 3181 aagtgtacat aatattgagg aatcctgctt tcagtttctg aaatttaagt ttttggactc 3241 cactgcagac cagcaagaat gcccaagaaa aaaatgcttt tcatcacact gtcagaaaac 3301 agaccttaaa ctttcacttt tggaccagag ggatctagaa actgatgaag tggaggaatt 3361 tctggaaaat aaaaatgttc agactcctca gtgtaaactc cgcaggtatc aaggaaatgc 3421 aaaagcctca cctcctctac aagacagtgc cagtcagaca tatgagtcca tgtgcttaga 3481 gaaggatgct gctctggcct tgccttcttt atgccccaaa tacagaaaat tccaaaaagc 3541 atttggaact gacagagtcc gtactgggga atctagtgtc aaagacattc atgcttctgt 3601 tcagccaaat gaaaggtctg aaaatgaatg cctgggagga gtcccggagt gtagagattt 3661 gcaggtgatg ttaaaatgtg acgaaagtaa attagcaatg gaacctgaag aaacgaagaa 3721 agatcctgct tctcagtgcc caactgaaaa atcagaagtg actcctttcc cccacaattc 3781 ttccatagac cctcatggac tttattcttt gtctctttta cacacatatg accaatatgg 3841 tgacttgaat tttgctggta tgcaaaacac aacagtgtta acagaaaagc ctttgtcagg 3901 tacagacgtc caagaaaaaa catttggtga aagtcaggat ttacctttga aatccgactt 3961 gggcaccagg gaagatagta gtgttgcatc tagtgatagg agtagtgtgg agcgagaagt 4021 ggcagaacac ctagcaaaag gcttctggag tgacatttgc agcacggaca ctccttgcca 4081 aatgcagtta tcacctgctg tggccaaaga tggctcagaa cagatctcac agaaacggtc 4141 tgagtgtccg tggttaggta tcaggattag tgagagccca gaaccaggtc aaaggacttt 4201 cacaacatta agttctgtca actgcccttt tataagtact ctgagtactg aaggctgttc 4261 aagcaatttg gaaattggaa acgatgatta tgtttcagaa ccccagcaag aaccttgccc 4321 atatgcttgt gtcattagct tgggagacga ctctgagacg gacaccgaag gagacagtga 4381 atcctgttca gccagagaac aagaatgtga ggtaaaactg ccattcaatg cacaacggat 4441 aatttcactg tctcgaaatg attttcagtc cttgttgaaa atgcacaagc ttactccaga 4501 acagctggat tgtatccatg atattcgaag aagaagtaaa aacagaattg ctgcacagcg 4561 ctgtcgcaag agaaaacttg actgtataca gaatcttgaa tcagaaattg agaagctgca 4621 aagtgaaaag gagagcttgt tgaaggaaag agatcacatt ttgtcaactc tgggtgagac 4681 aaagcagaac ctaactggac tttgccagaa agtttgtaaa gaagcagctc tgagtcaaga 4741 acaaatacag atactcgcca agtactcagc tgcagattgc ccactttcat ttttaatttc 4801 tgaaaaagat aaaagtactc ctgatggtga actggcgtta ccatcaattt tcagtttatc 4861 tgaccggcct ccagcagtgc tgcctccctg tgccagagga aacagtgagc ctggctacgc 4921 gcgagggcag gagtcccagc agatgtccac agccacctct gagcaagctg ggcctgcgga 4981 acagtgtcgt cagagtggtg ggatctcaga tttctgtcag cagatgactg ataaatgtac 5041 tactgatgag ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga 5101 ctacaaggat gacgatgaca agtagtctag ataattctac cgggtagggg aggcgctttt 5161 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag 5221 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt 5281 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc 5341 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca 5401 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca 5461 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct 5521 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg 5581 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc 5641 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc 5701 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 5761 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 5821 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 5881 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 5941 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 6001 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 6061 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 6121 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 6181 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 6241 acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg 6301 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 6361 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 6421 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 6481 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 6541 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 6601 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 6661 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 6721 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6781 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 6841 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat 6901 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 6961 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 7021 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta 7081 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 7141 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga 7201 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag 7261 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg 7321 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg 7381 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt 7441 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa 7501 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct 7561 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7621 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7681 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7741 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt 7801 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 7861 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 7921 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 7981 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 8041 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 8101 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 8161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 8221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 8281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 8341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 8401 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 8461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 8521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 8581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 8641 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 8701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 8761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 8821 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 8881 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 8941 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 9001 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 9061 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 9121 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 9181 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 9241 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 9301 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 9361 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 9421 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 9481 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 9541 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 9601 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 9661 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 9721 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 9781 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 9841 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 9901 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 9961 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca 10021 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 10081 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 10141 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc 10201 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca 10261 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt 10321 gtatttgtta aatatgtact acaaacttag tagt //