LOCUS       V042369                11281 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042369
VERSION     V042369
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5263
                     /label="Arpp21(NM_033264)"
                     /note="Arpp21(NM_033264)"
                     /gene="Arpp21"
     CDS             5264..5320
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5333..6052
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        6069..6568
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6589..7188
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7202..7790
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7997..8630
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8759..8775)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8783..8799
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8807..8837)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8852..8873
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9161..9749)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9920..10780)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10781..10885)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10933..11067
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgtctga gcaaggagga ctgactccga ccatactgga
     2881 agaagggcag acggagccag agtctgcccc agaaaatggc atcctcaagt cagaaagtct
     2941 ggatgaggag gagaagctgg aactgcagcg gcgactggcg gctcagaacc aagagaggag
     3001 aaaatccaag tcaggagcag gcaaagggaa gctgaccaga agtcttgctg tctgtgaaga
     3061 gtcttcagct agatctggag gggaaagtca ccaggatcag gaatcaattc acttacagct
     3121 ttccagtttc cccagcctgc aagaggagga taaatctagg aaggatgatt ctgagagaga
     3181 aaaagaaaag gataagaaca gagagaaact ctctgagaga cccaagatca gaatgttatc
     3241 aaaagattgc agccaagaat acacagattc tacaggcata gacttacatg ggtttctaat
     3301 taacacgctg aagaacaatt ccagggacag gatgatactc ttgaaaatgg agcaggaaat
     3361 gattgatttc attgctgaca gcaataacca ctataaaaag ttcccccaga tgtcatccta
     3421 tcaaaggatg ctggtccatc gggtggcagc gtactttgga ttggaccaca acgtggatca
     3481 aactggaaaa tctgtcatca tcaacaagac cagcagcacc aggataccag agcaaaggtt
     3541 ttgtgaacat ttaaaagatg aaaaaagtga agaatcccag aagcggttta tcttgaagcg
     3601 agataactct agtattgata aagaagacaa tcagaacaga atgcacccat ttagagatga
     3661 cagacgaagt aaatcaattg aagagagaga agaggaatat cagagagtga gggagagaat
     3721 atttgcacat gattcagttt gctcacagga aagcctattt ttggacaaca gtaggctcca
     3781 ggaagacatg cacatatgca atgagaccta taagaaaaga cagctctttc gggctcaccg
     3841 agacagctca ggaagaactt ccgggagccg gcagagcagc tcagagactg agctcaggtg
     3901 gccagaccac cagcgggctt ggagcagcac agattcggac agttccaacc gcaatctgaa
     3961 gcccaccatg accaagacgg caagttttgg gggcatcacg gtgctgacca ggggcgacag
     4021 cacgtccagt accaggagtg ctgggaaact gtccaaaaca ggttctgagt cttccagcag
     4081 tgcgggctcc tcaggatcac tgtcccgcac ccacccacag agcacggccc tgacctctag
     4141 cgtggcagct ggttctcctg gctgtatggc ctattcagag aatggaatgg gaggccaggt
     4201 tcctcccagc agcaccagct acatcctcct cccacttgaa agtgcgactg gcatcccacc
     4261 cggaagcatc cttcttaatc cacacacagg tcagcccttt gtgaacccgg atggaacgcc
     4321 tgcgatatac aaccctcccg gaagtcagca gacactgcgt ggcactgttg gtgggcagcc
     4381 ccagcaacct ccacagcagc aaccttcgcc tcagcctcag cagcaggtcc aggcatcaca
     4441 gccccagatg gcagggccac tggtcactca gagagaagaa ctggctgccc agttcagcca
     4501 gctgagcatg agccggcagt cctcgggaga cactcctgaa cctccctctg gtacagtcta
     4561 cccagcctct ctcctgccac aaacggctca gccacagagt tatgtcatca cttctgcagg
     4621 ccagcagctc tctacagggg gcttctcgga ctctggcccg cccatctccc agcaggtcct
     4681 gcaggccccg ccctctcccc agggatttgt acagcagcct ccacctgcac agatgtccgt
     4741 ctattactac ccatctggtc agtaccctac ctcaacctca cagcagtacc gacccctcgc
     4801 ctcagtccag tacagtgctc agcggagtca gcaaatacca cagaccacac agcaagcagg
     4861 ttaccagcca gtcttgtctg gtcagcaggg cttccaaggc atgatgggag tacagcagtc
     4921 agcgcacagc cagggtgtga tgagcagtca gcaaggagcc ccggtgcatg gtgtgatggt
     4981 ctcctaccca accatgtctt cttatcaggt gccaatgacc caaggctctc aagcagtgcc
     5041 ccagcagaca taccaaccac caatcatgct gcccagtcag gcaggccaag ggtcactccc
     5101 agccaccgga atgcctgtgt actgtaacgt cacaccgcca aaccctcaga acaacctaag
     5161 gttgatgggt ccacactgcc cctccagcac ggttcctgtc atgtccgcta gttgcagaac
     5221 aaactgtggg aacgtgagca acgcaggctg gcaggtcaag ttcgccacta acttctccct
     5281 gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca ggatccgcca ccatggtgag
     5341 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5401 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5461 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5521 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     5581 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5641 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5701 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5761 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5821 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5881 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5941 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     6001 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt agtctagata
     6061 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     6121 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     6181 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     6241 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     6301 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     6361 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     6421 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     6481 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     6541 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac
     6601 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6661 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6721 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6781 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6841 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6901 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6961 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     7021 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     7081 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     7141 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa
     7201 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7261 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7321 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7381 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7441 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7501 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7561 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7621 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7681 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7741 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     7801 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     7861 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     7921 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7981 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     8041 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     8101 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     8161 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     8221 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     8281 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     8341 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     8401 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     8461 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8521 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8581 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8641 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8701 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt
     8761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8821 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    10921 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10981 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    11041 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    11101 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    11161 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    11221 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    11281 t
//
