LOCUS       V044585                10300 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044585
VERSION     V044585
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4975
                     /label="Acsl4(NM_207625)"
                     /note="Acsl4(NM_207625)"
                     /gene="Acsl4"
     CDS             4982..5008
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5012..5038
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5042..5068
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5088..5587
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5608..6207
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6221..6809
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7016..7649
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7778..7794)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7802..7818
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7826..7856)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7871..7892
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8180..8768)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8939..9799)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9800..9904)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9952..10086
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgaacct taagctaaat gtgctcacca ttatattgct
     2881 gcctgtccac ttgttaataa caatatacag tgcccttata tttattccat ggtattttct
     2941 taccaatgcc aagaagaaaa acgctatggc aaagagaata aaagctaagc ccacttcaga
     3001 caaacctgga agtccatatc gctctgtcac acacttcgac tcactagctg tcatagacat
     3061 ccctggagca gatactctgg ataaattatt tgaccatgct gtagccaaat ttgggaagaa
     3121 ggacagcctt ggaacccggg agatcctgag tgaagaaaat gaaatgcagc caaatggaaa
     3181 ggtttttaag aagttaattc ttgggaatta taaatggata aactatcttg aagtgaactg
     3241 cagagtgaat aactttggaa gtggcctcac tgcattggga ctgaaaccaa agaacaccat
     3301 tgccattttc tgtgagacca gggcagagtg gatgattgca gcacagactt gctttaagta
     3361 caactttcca cttgtgactt tatatgccac acttggcaga gaagctgtag ttcatggatt
     3421 aaatgaatct gaggcttcct atctgattac tagtgttgag cttctggaaa gcaaactgaa
     3481 ggcggcctta gtagatatca attgtgttaa acatatcatt tatgtggata ataagactat
     3541 caatagagca gagtaccctg aggggcttga aattcacagc atgcaatcag tagaggagct
     3601 gggagccaag ccagaaaact tgagcgttcc tccaagtaga ccaacccctt cagacatggc
     3661 cattgtcatg tacaccagtg gttctacggg ccgccccaag ggagtgatga tgcatcatag
     3721 caatttgatt gctggaatga caggccagtg tgaacgtatc cctggactag gaccgaagga
     3781 cacatatatt ggctacttac ctttggctca tgtgctggaa ctgacagcag agatatcatg
     3841 ctttacctat ggctgtagga ttggatactc ttcacccctt acactgtctg accagtccag
     3901 caaaatcaag aagggaagca agggtgattg tactgtactg aaacccacac ttatggccgc
     3961 tgttccggaa atcatggata gaatttataa gaatgttatg agcaaggttc aagagatgaa
     4021 ttatgttcag aaaactctat ttaaaatcgg gtatgattac aaattagagc aaatcaagaa
     4081 aggctatgac gcccctcttt gtaatctgat actgtttaaa aaggtgaagg ctctgctggg
     4141 agggaatgtc cgcatgatgc tgtctggcgg cgcgccactg tcccctcaga cacaccgatt
     4201 catgaatgtc tgcttctgct gccccattgg tcagggatat gggctgacag aatcatgtgg
     4261 tgctggaaca gttactgaag ttactgacta cactactgga agagttggag ctcctcttat
     4321 ttgctgtgaa attaaactga aagactggca ggaaggtggt tatacagttc atgataagcc
     4381 gaaccccaga ggtgagattg tgatcggtgg ccagaatatc tccatgggat attttaaaaa
     4441 cgaagagaaa acagcagaag attattgtgt tgatgaaaat ggacaaaggt ggttttgcac
     4501 tggcgatatt ggagaattcc atcctgatgg atgcttacag attatagatc gtaagaaaga
     4561 tctggtaaag ttacaagcag gagaatatgt atctcttggg aaagtagaag ctgcactgaa
     4621 gaattgtcca ctgatcgaca acatctgtgc ttttgccaaa agtgaccagt cctatgtgat
     4681 cagttttgtg gttcctaacc agaaaaagtt gactcttttg gcacaacaga agggggtaga
     4741 aggatcttgg gttgatattt gcaataatcc cgccatggaa gctgaaatac tgaaagaaat
     4801 tcgagaagct gcaaatgcca tgaaattgga gcgatttgaa attccgatca aggttcggtt
     4861 aagcccagag ccatggaccc ccgagactgg tttggtaaca gatgccttca agctgaaaag
     4921 gaaggagttg aagaaccatt atctcaaaga cattgagcga atgtatgggg gcaaaggatc
     4981 ctacccatac gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag
     5041 ctatccttat gatgtcccag actacgcata gtctagataa ttctaccggg taggggaggc
     5101 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5161 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5221 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5281 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5341 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     5401 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     5461 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     5521 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5581 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     5641 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5701 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5761 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5821 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5881 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5941 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6001 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6061 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6121 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6181 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa
     6241 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6301 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6361 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6421 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6481 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6541 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6601 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6661 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6721 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6781 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     6841 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     6901 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     6961 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     7021 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     7081 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     7141 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     7201 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     7261 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     7321 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     7381 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     7441 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     7501 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7561 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7621 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7681 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
     7741 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7801 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7861 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7921 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7981 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8581 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9901 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
     9961 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10021 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10081 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    10141 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    10201 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa
    10261 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
