LOCUS       V045164                10327 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045164
VERSION     V045164
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5002
                     /label="Acsl3(NM_028817)"
                     /note="Acsl3(NM_028817)"
                     /gene="Acsl3"
     CDS             5009..5035
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5039..5065
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5069..5095
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5115..5614
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5635..6234
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6248..6836
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7043..7676
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7805..7821)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7829..7845
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7853..7883)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7898..7919
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8207..8795)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8966..9826)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9827..9931)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9979..10113
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgaataa ccacgtatct tcaacaccat caaccatgaa
     2881 gctaaaacaa accatcaacc ccatactttt atatttcata cattttataa tatcactcta
     2941 tactatttta acatacatcc cattttattt tttgtgtgag tcaaaacaag agaaaccaaa
     3001 ccaaattaaa gcaaaacctg tcagttccaa accggactct gcatacagat ctatcaacag
     3061 tgtggatggc ttggcttcag tgctgtatcc tggctgcgat acacttgata aagtctttat
     3121 gtatgcaaaa aacaaattta aaaacaaaag actattggga acacgtgaaa ttttaaatga
     3181 ggaagatgaa atacagccaa atggaaaaat ttttaaaaag gttattctgg ggcactataa
     3241 ttggctttcc tatgaagatg tcttcatccg agcccttgac tttggaaatg ggttacaaat
     3301 gttgggccag aaaccaaagg ccaacatcgc catcttctgt gagaccaggg ctgagtggat
     3361 gatcgctgca caggcgtgtt ttatgtataa cttccagctt gttacactgt atgctactct
     3421 gggaggtcca gccattgttc atggactgaa tgagacagag gtgaccaaca tcattactag
     3481 caaagaactc ttgcaaacaa agctgaagga tatagtctct ttggtcccac gtctgcggca
     3541 tatcattact gttgatggga agcctccaac ctggtctgag ttccccaaag gtgtcattgt
     3601 acacaccatg gctgcagtgc aggctctagg agtgaaggcc aacgtggaaa agaaagctca
     3661 cagcaaacca ctgccctcag atattgcagt aatcatgtac acaagtgggt ccacaggaat
     3721 tccaaaggga gtcatgatct cacacagcaa catcattgct tctataacgg ggatggcgag
     3781 aaggattcca agactgggag aggaagatgt gtatattggc tacttgcccc tggcacatgt
     3841 tctagaatta agtgctgagc ttgtgtgtct ttctcatgga tgccgaatcg gctactcttc
     3901 accacagaca ttagcagatc agtcttcaaa aataaaaaaa ggaagcaaag gagacacatc
     3961 cgtgctgaag ccaacactga tggcagctgt tccggaaatc atggatcgga tctacaaaaa
     4021 tgtcatgaat aaagtgaatg aaatgagtgc ttttcaacga aacttgttta ttttggcata
     4081 taattataag atggaacaga tttcaaaagg gtgtagtact ccactgtgtg accgctttgt
     4141 tttccggaat gtccgaaggc tgctgggtgg aaatattcgc cttttactgt gtggtggtgc
     4201 tccgctttct gcaacaacgc agcgattcat gaatatctgt ttctgctgtc ccgttggtca
     4261 ggggtatgga ctcacagaat ctactggggc tggaacaatt acagaagtgt gggactacaa
     4321 taccggcaga gtgggagcac cattagtttg ctgtgaaatc aaattaaaga actgggagga
     4381 aggtggctat tttaatactg acaaaccaca tcccagaggt gaaattctta ttggtggcca
     4441 aaatgtgaca atggggtact acaaaaatga agcaaaaaca aagacagatt tctttgaaga
     4501 tgaaaatgga cagcggtggc tgtgcactgg agatattgga gagtttgacc ctgacggctg
     4561 tctgaagatc attgaccgta aaaaggacct tgtgaaacta caggcaggag agtacgtttc
     4621 tctcgggaaa gtagaggcag ctttgaagaa cctcccactg atagataaca tttgtgcgta
     4681 tgcaaacagc taccattctt acgtaattgg gtttgttgtg ccaaatcaaa aggaacttac
     4741 agagctagct agaacaaaag gatttaaagg aacttgggaa gagctgtgta acagcagcga
     4801 gatggaaaat gaggtcctta aagtgctttc tgaagctgct atttcagcaa gtctggaaaa
     4861 gtttgaaatt ccactaaaaa tacgtttgag ccctgaccca tggactcccg aaactggtct
     4921 ggtgacagat gccttcaagt tgaaacgtaa agagcttaaa acacactacc aggcagacat
     4981 tgagcggatg tatggaagaa aaggatccta cccatacgac gtacctgatt atgcaactta
     5041 tccttatgat gtacctgatt atgctagcta tccttatgat gtcccagact acgcatagtc
     5101 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     5161 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     5221 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     5281 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     5341 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     5401 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     5461 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     5521 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     5581 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
     5641 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5701 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5761 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5821 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5881 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5941 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6001 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6061 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6121 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6181 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt
     6241 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6301 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6361 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6421 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6481 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6541 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6601 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     6661 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6721 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6781 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6841 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     6901 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     6961 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7021 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc
     7081 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     7141 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7201 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     7261 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     7321 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     7381 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     7441 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7681 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7741 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat
     7801 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7861 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7921 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8641 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9961 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10081 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    10141 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
    10201 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    10261 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10321 tagtagt
//
