LOCUS V039778 10306 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039778 VERSION V039778 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5002 /label="Acsl3(NM_028817)" /note="Acsl3(NM_028817)" /gene="Acsl3" CDS 5009..5074 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5094..5593 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5614..6213 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6227..6815 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7022..7655 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7784..7800) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7808..7824 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7832..7862) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7877..7898 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8186..8774) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8945..9805) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9806..9910) /gene="bla" /label="AmpR promoter" polyA_signal 9958..10092 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgaataa ccacgtatct tcaacaccat caaccatgaa 2881 gctaaaacaa accatcaacc ccatactttt atatttcata cattttataa tatcactcta 2941 tactatttta acatacatcc cattttattt tttgtgtgag tcaaaacaag agaaaccaaa 3001 ccaaattaaa gcaaaacctg tcagttccaa accggactct gcatacagat ctatcaacag 3061 tgtggatggc ttggcttcag tgctgtatcc tggctgcgat acacttgata aagtctttat 3121 gtatgcaaaa aacaaattta aaaacaaaag actattggga acacgtgaaa ttttaaatga 3181 ggaagatgaa atacagccaa atggaaaaat ttttaaaaag gttattctgg ggcactataa 3241 ttggctttcc tatgaagatg tcttcatccg agcccttgac tttggaaatg ggttacaaat 3301 gttgggccag aaaccaaagg ccaacatcgc catcttctgt gagaccaggg ctgagtggat 3361 gatcgctgca caggcgtgtt ttatgtataa cttccagctt gttacactgt atgctactct 3421 gggaggtcca gccattgttc atggactgaa tgagacagag gtgaccaaca tcattactag 3481 caaagaactc ttgcaaacaa agctgaagga tatagtctct ttggtcccac gtctgcggca 3541 tatcattact gttgatggga agcctccaac ctggtctgag ttccccaaag gtgtcattgt 3601 acacaccatg gctgcagtgc aggctctagg agtgaaggcc aacgtggaaa agaaagctca 3661 cagcaaacca ctgccctcag atattgcagt aatcatgtac acaagtgggt ccacaggaat 3721 tccaaaggga gtcatgatct cacacagcaa catcattgct tctataacgg ggatggcgag 3781 aaggattcca agactgggag aggaagatgt gtatattggc tacttgcccc tggcacatgt 3841 tctagaatta agtgctgagc ttgtgtgtct ttctcatgga tgccgaatcg gctactcttc 3901 accacagaca ttagcagatc agtcttcaaa aataaaaaaa ggaagcaaag gagacacatc 3961 cgtgctgaag ccaacactga tggcagctgt tccggaaatc atggatcgga tctacaaaaa 4021 tgtcatgaat aaagtgaatg aaatgagtgc ttttcaacga aacttgttta ttttggcata 4081 taattataag atggaacaga tttcaaaagg gtgtagtact ccactgtgtg accgctttgt 4141 tttccggaat gtccgaaggc tgctgggtgg aaatattcgc cttttactgt gtggtggtgc 4201 tccgctttct gcaacaacgc agcgattcat gaatatctgt ttctgctgtc ccgttggtca 4261 ggggtatgga ctcacagaat ctactggggc tggaacaatt acagaagtgt gggactacaa 4321 taccggcaga gtgggagcac cattagtttg ctgtgaaatc aaattaaaga actgggagga 4381 aggtggctat tttaatactg acaaaccaca tcccagaggt gaaattctta ttggtggcca 4441 aaatgtgaca atggggtact acaaaaatga agcaaaaaca aagacagatt tctttgaaga 4501 tgaaaatgga cagcggtggc tgtgcactgg agatattgga gagtttgacc ctgacggctg 4561 tctgaagatc attgaccgta aaaaggacct tgtgaaacta caggcaggag agtacgtttc 4621 tctcgggaaa gtagaggcag ctttgaagaa cctcccactg atagataaca tttgtgcgta 4681 tgcaaacagc taccattctt acgtaattgg gtttgttgtg ccaaatcaaa aggaacttac 4741 agagctagct agaacaaaag gatttaaagg aacttgggaa gagctgtgta acagcagcga 4801 gatggaaaat gaggtcctta aagtgctttc tgaagctgct atttcagcaa gtctggaaaa 4861 gtttgaaatt ccactaaaaa tacgtttgag ccctgaccca tggactcccg aaactggtct 4921 ggtgacagat gccttcaagt tgaaacgtaa agagcttaaa acacactacc aggcagacat 4981 tgagcggatg tatggaagaa aaggatccga ctacaaagac catgacggtg attataaaga 5041 tcatgacatc gactacaagg atgacgatga caagtagtct agataattct accgggtagg 5101 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 5161 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 5221 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 5281 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 5341 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 5401 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 5461 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 5521 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 5581 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 5641 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5701 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5761 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5821 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5881 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5941 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 6001 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 6061 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 6121 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 6181 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 6241 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 6301 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 6361 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 6421 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 6481 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 6541 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 6601 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 6661 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 6721 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 6781 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 6841 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 6901 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 6961 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 7021 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 7081 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 7141 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 7201 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 7261 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 7321 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 7381 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 7441 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 7501 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 7561 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 7621 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 7681 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 7741 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 7801 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 7861 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 7921 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 7981 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 8041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 8101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 8161 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 8221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 8281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 8341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 8401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 8461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 8521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 8581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 8641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 8701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 8761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 8821 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 8881 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 8941 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 9001 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 9061 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 9121 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 9181 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 9241 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 9301 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 9361 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 9421 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 9481 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 9541 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 9601 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 9661 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 9721 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 9781 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 9841 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 9901 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 9961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 10021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 10081 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 10141 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 10201 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt 10261 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //