LOCUS       V020267                11653 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020267
VERSION     V020267
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..6189
                     /label="Tert(NM_053423)"
                     /note="Tert(NM_053423)"
                     /gene="Tert"
     misc_feature    6227..6813
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             6814..7608
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7622..8210
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8417..9050
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9178..9194)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9202..9218
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9226..9256)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9271..9292
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9580..10168)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10339..11199)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11200..11304)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11352..11486
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgccc
     2821 cgcgctcctc gttgccccgc cgtgcgctct ctactgcgca gccgatatcg ggaggtgtgg
     2881 ccgctggcga cctttgtgcg gcgcctgggg cttgagggca gtcggcttgt gcaacccggg
     2941 gacccgaagg tcttccgcac gttggttgcc cagtgcctag tgtgcgtgcc ctggggctca
     3001 cagccgccac ctgctgacct ttccttccac caggtgtcat ccctgaaaga gctggtgtcc
     3061 agggttgtgc agaaactttg cgagcgcggt gagaggaatg tgctggcttt tggctttgca
     3121 ctgcttaacg gggccagagg tgggcctccc atggccttca cgaccagcgt gcatagctac
     3181 ttgcccaact cggttactga gtccctgtgt gtcagtggtg catggatgct actgttgagc
     3241 cgagtgggcg acgacctgct ggtctacctg ctgtcgcact gtgcgctcta cctgctggtg
     3301 ccccccagct gtgcctacca ggtgtgcggg tcacccctgt accaaatttg tgccaccacg
     3361 gatacctggt cctctgtgcc cgctggttac aggcccactc gacccgtggg cgggaatttc
     3421 actaaccttg ggtccgcaca ccagatcaaa aacagtggtc accaggaagc accaaaaccc
     3481 caggccttgc catcacgagg tacgaagagg cttctgagtc tcaccagtac aaacgtgcct
     3541 tcagctaaga aggccaggtt tgaacctgcc ctgagagtgg ataagggacc ccacaggcag
     3601 gtggtaccaa ccccatcagg caaaacatgg gcgccaagtc ctgctgcgtc ccccaaggtg
     3661 cctcctgcag cgaaaaactt gtctttgaaa ggaaaggcat ctgacccgag tctctctggg
     3721 tcggtgtgct gtaaacacaa gcccagctcc tcgtccctgc tgtcatcacc accccaagat
     3781 gctgaaaagc tcaggccatt cactgagacc agacatttcc tttactccag gggaggtggc
     3841 caagaggagc taaatccctc attcctactc aacagcctcc cgcctagctt gaccggggcc
     3901 aggagactgg tggagatcat ctttctgggc tcaaggccta ggacatcagg accattctgc
     3961 aggacccgcc gcctgccccg tcgatactgg cagatgcgac ccctattcca gcagctgctc
     4021 atgaaccacg caaagtgcca atatgtcaga ttcctccggt cgcactgcag atttcgaaca
     4081 gcaaaccagc gggtgccgga tgccatggac accagcccat cccacctcac gagtttgctc
     4141 cggttacaca gcagcccctg gcaggtatac ggctttcttc gggcctgcct ccgcgagctg
     4201 gtgcctgccg gtctctgggg caccaggcac aatgagcgcc gcttcttaaa gaacgtgaag
     4261 aagttcatct cgttggggaa gtacgccaag ctatccctgc aggaactgat gtggagggtg
     4321 aaagtggagg actgccactg gctccgcagc agcccagaga aggacactgt ccctgccgca
     4381 gagcaccgtc tgagggagag gatccttgcc atgttcctgt tctggctaat ggacacatat
     4441 gtggtacagc tgctgaggtc attcttctac atcacagaga ccacgttcca gaagaaccgc
     4501 cttttcttct accgtaagag tgtgtggagc aagctgcaga gcattggaat caggcaacag
     4561 cttgagagag ttcagctacg ggaactgtca caagaggagg tcaagcatca ccaggacact
     4621 tggctggcca tgcctatctg cagattgcgc ttcatcccca agctcaatgg tctccggccc
     4681 attgtgaaca tgagttatgg catggacacc agagcttttg gcaaaaagaa gcagacccag
     4741 tgtttcactc agagtctcaa gactttgttc agcgtgctca actacgagcg gaccaaacat
     4801 cctaacctta tgggtgcttc agtactgggt acgagtgaca gctacaggat ctggcggacc
     4861 ttcgtgctgc gtgtgcgtgc tctggaccag acacccagga tgtactttgt taaggcagat
     4921 gtgacagggg cctatgatgc catcccccag gacaagctcg tggaaattgt cgccaatata
     4981 atcaggcgct cagagagcat gtactgtatc cgccagtatg cagtggttca gaaagatagc
     5041 caaggccaag tccacaagtc cttcaggaga caggtctcca ccctctctga cctccagcca
     5101 tacatgggcc agttcaccaa gcatctgcag gactcagatg ccagtgcact gaggaactct
     5161 gttgtcatcg agcagagcat ctccatgaat gagactggca gtagcctgct ccacttcttc
     5221 ctgcgctttg tccgtcacag tgtcgtgaag atcgatggca ggttctatgt gcaatgccag
     5281 ggcatccccc agggctccag cctgtccacc ctgctctgca gtctgtgttt cggagacatg
     5341 gagaacaagc tgtttgccga ggtgcagcag gacggcttgc ttttacgttt tgtcgatgac
     5401 tttctgttgg tgacacctca cctggcccat gcaaaagcct ttctcagcac cctggtccat
     5461 ggcgtgcccg agtatggctg catgataaac ttgcagaaga cagtggtgaa cttccctgtg
     5521 gagaccggcg ccctgggagg tgcagccccg caccagctgc ctgctcactg cctgtttccc
     5581 tggtgtggct tactgctgga cactcggact ttggaagtat tctgtgacta ctcaggttac
     5641 ggacggacct caattaagat gagcctcacc ttccagggtg tctccagggc cgggaagacc
     5701 atgcggtaca agctcttgtc agtcttgcgg ttgaagtgtc atggtctgtt tctagacttg
     5761 caggtgaaca gcctgcagac agtctgcatc aatatataca agatcttcct gcttcaggcc
     5821 tacaggttcc atgcatgtgt gattcggctt ccctttggcc agcatgttag gaagaaccat
     5881 gcattctttc tgggcatcat ctccaaccta gcatcctgct gctacgccat cctgaaggtc
     5941 aagaatccag gagtgtcact aagggccaag ggtgcccctg gctcctttcc gcccgaggcc
     6001 acacgttggc tctgctacca agccttcctg ctcaagctgg ctgctcattc tgtcacctac
     6061 aagtgtctcc tgggacctct taggacagcc caaaaacagc tgtgccggaa gctcccagag
     6121 gcaacaatga ccctccttaa gactgcagct gacccagccc taagcacaga ttttcagacc
     6181 attttggact aactcgagac tagttctaga gcggccgcgg atcccgcccc tctccctccc
     6241 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     6301 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     6361 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     6421 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     6481 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     6541 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     6601 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     6661 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     6721 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     6781 tttgaaaaac acgatgataa gcttgccaca accatggctg aacaagatgg attgcacgca
     6841 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6901 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6961 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7021 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7081 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7141 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7201 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7261 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7321 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     7381 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7441 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7501 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7561 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaac gcgtctggaa
     7621 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7681 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7741 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7801 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7861 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7921 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7981 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     8041 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8101 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8161 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     8221 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     8281 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     8341 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8401 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     8461 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     8521 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     8581 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     8641 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     8701 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     8761 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     8821 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     8881 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8941 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9001 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     9061 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9121 gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     9181 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     9241 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     9301 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     9361 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    10021 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    10081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    10141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    10201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    10261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    10321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    10381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    11041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    11101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    11161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    11221 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    11281 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    11341 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11401 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11461 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    11521 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11581 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11641 caaacttagt agt
//
