LOCUS V018454 9197 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018454 VERSION V018454 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2821..3759 /label="HOXA11(NM_005523)" /note="HOXA11(NM_005523)" /gene="HOXA11" misc_feature 3771..4357 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 4358..5152 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" misc_feature 5166..5754 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5961..6594 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6722..6738) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6746..6762 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6770..6800) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6815..6836 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7124..7712) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7883..8743) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8744..8848) /gene="bla" /label="AmpR promoter" polyA_signal 8896..9030 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgaggccacc 2821 atggattttg atgagcgtgg tccctgctcc tctaacatgt atttgccaag ttgtacttac 2881 tacgtctcgg gtccagattt ctccagcctc ccttcttttc tgccccagac cccgtcttcg 2941 cgcccaatga catactccta ctcctccaac ctgccccagg tccaacccgt gcgcgaagtg 3001 accttcagag agtacgccat tgagcccgcc actaaatggc acccccgcgg caatctggcc 3061 cactgctact ccgcggagga gctcgtgcac agagactgcc tgcaggcgcc cagcgcggcc 3121 ggcgtgcctg gcgacgtgct ggccaagagc tcggccaacg tctaccacca ccccaccccc 3181 gcagtctcgt ccaatttcta tagcaccgtg ggcaggaacg gcgtcctgcc acaggctttc 3241 gaccagtttt tcgagacagc ctacggcacc ccggaaaacc tcgcctcctc cgactacccc 3301 ggggacaaga gcgccgagaa ggggcccccg gcggccacgg cgacctccgc ggcggcggcg 3361 gcggctgcaa cgggcgcgcc ggcaacttca agttcggaca gcggcggcgg cggcggctgc 3421 cgggagacgg cggcggcagc agaggagaaa gagcggcggc ggcgccccga gagcagcagc 3481 agccccgagt cgtcttccgg ccacactgag gacaaggccg gcggctccag tggccaacgc 3541 acccgcaaaa agcgctgccc ctataccaag taccagatcc gagagctgga acgggagttc 3601 ttcttcagcg tctacattaa caaagagaag cgcctgcaac tgtcccgcat gctcaacctc 3661 actgatcgtc aagtcaaaat ctggtttcag aacaggagaa tgaaggaaaa aaaaattaac 3721 agagaccgtt tacagtacta ctcagcaaat ccactcctct aaggatcccg cccctctccc 3781 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 3841 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 3901 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 3961 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 4021 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 4081 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 4141 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag 4201 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt 4261 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 4321 ttcctttgaa aaacacgatg ataagcttgc cacaaccatg gctgaacaag atggattgca 4381 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 4441 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 4501 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 4561 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 4621 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 4681 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 4741 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 4801 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 4861 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 4921 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 4981 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 5041 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 5101 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaacgcgtct 5161 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 5221 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 5281 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 5341 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 5401 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 5461 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 5521 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 5581 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 5641 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 5701 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 5761 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc 5821 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt 5881 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 5941 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct 6001 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg 6061 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc 6121 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct 6181 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc 6241 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc 6301 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg 6361 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc 6421 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6481 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6541 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6601 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6661 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat 6721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 6781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 6841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 6901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 6961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 7321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 7501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 7561 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 7621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 7681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 7741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 7801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 7861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 7921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 7981 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 8161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 8221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 8281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 8341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 8401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 8461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 8521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 8581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 8641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 8701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 8761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 8821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 8881 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 8941 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 9001 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 9061 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 9121 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 9181 actacaaact tagtagt //