LOCUS       V018776                11379 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018776
VERSION     V018776
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..6030
                     /label="RNF31(NM_017999)"
                     /note="RNF31(NM_017999)"
                     /gene="RNF31"
     misc_feature    6050..6622
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             6624..7334
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    7348..7936
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8143..8776
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8904..8920)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8928..8944
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8952..8982)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8997..9018
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9306..9894)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10065..10925)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10926..11030)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11078..11212
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgccg
     2821 ggggaggaag aggagcgggc cttcctggtg gcccgcgagg agctggcgag cgccctgagg
     2881 agggattccg ggcaggcgtt ttccctggag cagctccggc cgctactagc cagctctctg
     2941 ccgctagccg cccgctacct gcagctggac gccgcacgcc ttgtccgctg caacgctcat
     3001 ggggagcccc gaaactacct caacaccctg tccacggctc tgaacatcct ggagaaatac
     3061 ggccgcaacc ttctcagccc tcagcggcct cggtactggc gtggtgtcaa gtttaataac
     3121 cctgtctttc gcagcacggt ggatgctgtg caggggggcc gagatgtgct gcgattatat
     3181 ggctacacag aggagcaacc agatgggttg agcttccccg aagggcagga ggagccagat
     3241 gagcaccagg ttgctacagt cacactggaa gtactgctgc ttcggacaga gctcagcctg
     3301 ctattgcaga atactcatcc aagacagcag gcactggagc agctgttgga agacaaggtt
     3361 gaagatgata tgctgcagct ttcagaattt gaccccctat tgagagagat tgctcctggc
     3421 cccctcacca caccctctgt cccaggctcc actcctggtc cctgcttcct ctgtggttct
     3481 gccccaggca cactgcactg cccatcctgt aaacaggccc tgtgtccagc ctgtgaccac
     3541 ctgttccatg gacacccatc ccgtgctcat cacctccgcc agaccctgcc tggggtcctg
     3601 cagggtaccc acctgagccc cagtttacct gcctcagccc aaccacggcc ccagtcgacc
     3661 tccctgctgg ccctgggaga cagctctctt tcttccccta atcctgcaag tgctcatttg
     3721 ccctggcact gtgctgcctg tgccatgcta aatgagcctt gggcagtgct ctgtgtggcc
     3781 tgtgatcggc cccgaggctg taaggggttg gggttgggaa ctgagggtcc ccaaggaact
     3841 ggaggcctag aacctgatct tgcacggggt cggtgggcct gccagagctg tacctttgag
     3901 aatgaggcag ctgctgtgct atgttccata tgtgagcgac ctcggctggc ccagcctccc
     3961 agcttggtgg tggattcccg agatgctggc atttgcctgc aaccccttca gcagggggat
     4021 gctttgctgg cctctgccca gagtcaagtc tggtactgta ttcactgtac cttctgcaac
     4081 tcgagccctg gctgggtgtg tgttatgtgc aaccggacta gtagccccat tccagcacaa
     4141 catgcccccc ggccctatgc cagctctttg gaaaagggac cccccaagcc tgggccccca
     4201 cgacgcctta gtgcccccct gcccagttcc tgtggagatc ctgagaagca gcgccaagac
     4261 aagatgcggg aagaaggcct ccagctagtg agcatgatcc gggaagggga agccgcaggt
     4321 gcctgtccag aggagatctt ctcggctctg cagtactcgg gcactgaggt gcctctgcag
     4381 tggttgcgct cagaactgcc ctacgtcctg gagatggtgg ctgagctggc tggacagcag
     4441 gaccctgggc tgggtgcctt ttcctgtcag gaggcccgga gagcctggct ggatcgtcat
     4501 ggcaaccttg atgaagctgt ggaggagtgt gtgaggacca ggcgaaggaa ggtgcaggag
     4561 ctccagtctc taggctttgg gcctgaggag gggtctctcc aggcattgtt ccagcacgga
     4621 ggtgatgtgt cacgggccct gactgagcta cagcgccaac gcctagagcc cttccgccag
     4681 cgcctctggg acagtggccc tgagcccacc ccttcctggg atgggccaga caagcagagc
     4741 ctggtcaggc ggcttttggc agtctacgca ctccccagct ggggccgggc agagctggca
     4801 ctgtcactgc tgcaggagac acccaggaac tatgagttgg gggatgtggt agaagctgtg
     4861 aggcacagcc aggaccgggc cttcctgcgc cgcttgcttg cccaggagtg tgccgtgtgt
     4921 ggctgggccc tgccccacaa ccggatgcag gccctgactt cctgtgagtg caccatctgt
     4981 cctgactgct tccgccagca cttcaccatc gccttgaagg agaagcacat cacagacatg
     5041 gtgtgccctg cctgtggccg ccccgacctc accgatgaca cacagttgct cagctacttc
     5101 tctacccttg acatccagct tcgcgagagc ctagagccag atgcctatgc gttgttccat
     5161 aagaagctga ccgagggtgt gctgatgcgg gaccccaagt tcttgtggtg tgcccagtgc
     5221 tcctttggct tcatatatga gcgtgagcag ctggaggcaa cttgtcccca gtgtcaccag
     5281 accttctgtg tgcgctgcaa gcgccagtgg gaggagcagc accgaggtcg gagctgtgag
     5341 gacttccaga actggaaacg catgaacgac ccagaatacc aggcccaggg cctagcaatg
     5401 tatcttcagg aaaacggcat tgactgcccc aaatgcaagt tctcgtacgc cctggcccga
     5461 ggaggctgca tgcactttca ctgtacccag tgccgccacc agttctgcag cggctgctac
     5521 aatgcctttt acgccaagaa taaatgtcca gagcctaact gcagggtgaa aaagtccctg
     5581 cacggccacc accctcgaga ctgcctcttc tacctgcggg actggactgc tctccggctt
     5641 cagaagctgc tacaggacaa taacgtcatg tttaatacag agcctccagc tggggcccgg
     5701 gcagtccctg gaggcggctg ccgagtgata gagcagaagg aggttcccaa tgggctcagg
     5761 gacgaagctt gtggcaagga aactccagct ggctatgccg gcctgtgcca ggcacactac
     5821 aaagagtatc ttgtgagcct catcaatgcc cactcgctgg acccagccac cttgtatgag
     5881 gtggaagagc tggagacggc cactgagcgc tacctgcacg tacgccccca gcctttggct
     5941 ggagaggatc cccctgctta ccaggcccgc ttgttacaga agctgacaga agaggtaccc
     6001 ttgggacaga gtatcccccg caggcggaag taggcggccg cggatcccgc ccctctccct
     6061 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     6121 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     6181 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     6241 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     6301 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     6361 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     6421 ggatagttgt ggaaagagtc aaatggctca cctcaagcgt attcaacaag gggctgaagg
     6481 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     6541 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     6601 cctttgaaaa acacgatgat aatatggtga gcaagggcga ggaggataac atggccatca
     6661 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     6721 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     6781 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     6841 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     6901 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     6961 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     7021 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     7081 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     7141 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     7201 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     7261 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     7321 agctgtacaa gtgaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     7381 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7441 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7501 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7561 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7621 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7681 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7741 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7801 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7861 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7921 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     7981 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     8041 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     8101 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     8161 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     8221 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     8281 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     8341 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     8401 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     8461 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     8521 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8581 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8641 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8701 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8761 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8821 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
     8881 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8941 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9001 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9061 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11041 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
    11101 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11161 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    11221 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    11281 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    11341 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
