LOCUS V018776 11379 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018776 VERSION V018776 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2815..6030 /label="RNF31(NM_017999)" /note="RNF31(NM_017999)" /gene="RNF31" misc_feature 6050..6622 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES" CDS 6624..7334 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" misc_feature 7348..7936 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8143..8776 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8904..8920) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8928..8944 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8952..8982) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8997..9018 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9306..9894) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10065..10925) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10926..11030) /gene="bla" /label="AmpR promoter" polyA_signal 11078..11212 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgccg 2821 ggggaggaag aggagcgggc cttcctggtg gcccgcgagg agctggcgag cgccctgagg 2881 agggattccg ggcaggcgtt ttccctggag cagctccggc cgctactagc cagctctctg 2941 ccgctagccg cccgctacct gcagctggac gccgcacgcc ttgtccgctg caacgctcat 3001 ggggagcccc gaaactacct caacaccctg tccacggctc tgaacatcct ggagaaatac 3061 ggccgcaacc ttctcagccc tcagcggcct cggtactggc gtggtgtcaa gtttaataac 3121 cctgtctttc gcagcacggt ggatgctgtg caggggggcc gagatgtgct gcgattatat 3181 ggctacacag aggagcaacc agatgggttg agcttccccg aagggcagga ggagccagat 3241 gagcaccagg ttgctacagt cacactggaa gtactgctgc ttcggacaga gctcagcctg 3301 ctattgcaga atactcatcc aagacagcag gcactggagc agctgttgga agacaaggtt 3361 gaagatgata tgctgcagct ttcagaattt gaccccctat tgagagagat tgctcctggc 3421 cccctcacca caccctctgt cccaggctcc actcctggtc cctgcttcct ctgtggttct 3481 gccccaggca cactgcactg cccatcctgt aaacaggccc tgtgtccagc ctgtgaccac 3541 ctgttccatg gacacccatc ccgtgctcat cacctccgcc agaccctgcc tggggtcctg 3601 cagggtaccc acctgagccc cagtttacct gcctcagccc aaccacggcc ccagtcgacc 3661 tccctgctgg ccctgggaga cagctctctt tcttccccta atcctgcaag tgctcatttg 3721 ccctggcact gtgctgcctg tgccatgcta aatgagcctt gggcagtgct ctgtgtggcc 3781 tgtgatcggc cccgaggctg taaggggttg gggttgggaa ctgagggtcc ccaaggaact 3841 ggaggcctag aacctgatct tgcacggggt cggtgggcct gccagagctg tacctttgag 3901 aatgaggcag ctgctgtgct atgttccata tgtgagcgac ctcggctggc ccagcctccc 3961 agcttggtgg tggattcccg agatgctggc atttgcctgc aaccccttca gcagggggat 4021 gctttgctgg cctctgccca gagtcaagtc tggtactgta ttcactgtac cttctgcaac 4081 tcgagccctg gctgggtgtg tgttatgtgc aaccggacta gtagccccat tccagcacaa 4141 catgcccccc ggccctatgc cagctctttg gaaaagggac cccccaagcc tgggccccca 4201 cgacgcctta gtgcccccct gcccagttcc tgtggagatc ctgagaagca gcgccaagac 4261 aagatgcggg aagaaggcct ccagctagtg agcatgatcc gggaagggga agccgcaggt 4321 gcctgtccag aggagatctt ctcggctctg cagtactcgg gcactgaggt gcctctgcag 4381 tggttgcgct cagaactgcc ctacgtcctg gagatggtgg ctgagctggc tggacagcag 4441 gaccctgggc tgggtgcctt ttcctgtcag gaggcccgga gagcctggct ggatcgtcat 4501 ggcaaccttg atgaagctgt ggaggagtgt gtgaggacca ggcgaaggaa ggtgcaggag 4561 ctccagtctc taggctttgg gcctgaggag gggtctctcc aggcattgtt ccagcacgga 4621 ggtgatgtgt cacgggccct gactgagcta cagcgccaac gcctagagcc cttccgccag 4681 cgcctctggg acagtggccc tgagcccacc ccttcctggg atgggccaga caagcagagc 4741 ctggtcaggc ggcttttggc agtctacgca ctccccagct ggggccgggc agagctggca 4801 ctgtcactgc tgcaggagac acccaggaac tatgagttgg gggatgtggt agaagctgtg 4861 aggcacagcc aggaccgggc cttcctgcgc cgcttgcttg cccaggagtg tgccgtgtgt 4921 ggctgggccc tgccccacaa ccggatgcag gccctgactt cctgtgagtg caccatctgt 4981 cctgactgct tccgccagca cttcaccatc gccttgaagg agaagcacat cacagacatg 5041 gtgtgccctg cctgtggccg ccccgacctc accgatgaca cacagttgct cagctacttc 5101 tctacccttg acatccagct tcgcgagagc ctagagccag atgcctatgc gttgttccat 5161 aagaagctga ccgagggtgt gctgatgcgg gaccccaagt tcttgtggtg tgcccagtgc 5221 tcctttggct tcatatatga gcgtgagcag ctggaggcaa cttgtcccca gtgtcaccag 5281 accttctgtg tgcgctgcaa gcgccagtgg gaggagcagc accgaggtcg gagctgtgag 5341 gacttccaga actggaaacg catgaacgac ccagaatacc aggcccaggg cctagcaatg 5401 tatcttcagg aaaacggcat tgactgcccc aaatgcaagt tctcgtacgc cctggcccga 5461 ggaggctgca tgcactttca ctgtacccag tgccgccacc agttctgcag cggctgctac 5521 aatgcctttt acgccaagaa taaatgtcca gagcctaact gcagggtgaa aaagtccctg 5581 cacggccacc accctcgaga ctgcctcttc tacctgcggg actggactgc tctccggctt 5641 cagaagctgc tacaggacaa taacgtcatg tttaatacag agcctccagc tggggcccgg 5701 gcagtccctg gaggcggctg ccgagtgata gagcagaagg aggttcccaa tgggctcagg 5761 gacgaagctt gtggcaagga aactccagct ggctatgccg gcctgtgcca ggcacactac 5821 aaagagtatc ttgtgagcct catcaatgcc cactcgctgg acccagccac cttgtatgag 5881 gtggaagagc tggagacggc cactgagcgc tacctgcacg tacgccccca gcctttggct 5941 ggagaggatc cccctgctta ccaggcccgc ttgttacaga agctgacaga agaggtaccc 6001 ttgggacaga gtatcccccg caggcggaag taggcggccg cggatcccgc ccctctccct 6061 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 6121 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 6181 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 6241 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 6301 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 6361 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 6421 ggatagttgt ggaaagagtc aaatggctca cctcaagcgt attcaacaag gggctgaagg 6481 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta 6541 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt 6601 cctttgaaaa acacgatgat aatatggtga gcaagggcga ggaggataac atggccatca 6661 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 6721 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 6781 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 6841 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 6901 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 6961 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 7021 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 7081 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 7141 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 7201 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 7261 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 7321 agctgtacaa gtgaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga 7381 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 7441 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 7501 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 7561 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 7621 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 7681 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 7741 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 7801 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 7861 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 7921 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa 7981 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg 8041 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg 8101 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact 8161 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg 8221 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct 8281 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca 8341 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt 8401 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact 8461 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga 8521 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg 8581 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 8641 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 8701 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 8761 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 8821 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct 8881 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 8941 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 9001 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 9061 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 9121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 9181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 9241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 9301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 9361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 9421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 9481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 9541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 9601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 9661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 9721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 9781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 9841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 9901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 9961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 10021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 10081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 10141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 10201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 10261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 10321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 10381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 10441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 10501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 10561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 10621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 10681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 10741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 10801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 10861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 10921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 10981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 11041 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa 11101 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 11161 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 11221 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 11281 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag 11341 aaattgtatt tgttaaatat gtactacaaa cttagtagt //