LOCUS V021284 9786 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021284 VERSION V021284 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2815..4437 /label="EHD4(NM_139265)" /note="EHD4(NM_139265)" /gene="EHD4" misc_feature 4457..5029 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES" CDS 5031..5741 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" misc_feature 5755..6343 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6550..7183 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7311..7327) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7335..7351 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7359..7389) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7404..7425 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7713..8301) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8472..9332) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9333..9437) /gene="bla" /label="AmpR promoter" polyA_signal 9485..9619 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgttc 2821 agctggatgg ggcggcaggc gggcgggcgc gaacgcgctg gcggcgcgga cgcggtgcag 2881 acggtgacgg gcgggctgcg ctcgctctac ctgcgcaagg tgctgccgct ggaggaggcg 2941 taccgcttcc acgagttcca ctcgcctgcg ctggaggacg ccgacttcga gaacaagccc 3001 atgatcctgc tggtgggcca gtacagcacc ggcaagacca ccttcatcag atacctactg 3061 gagcaggatt tcccaggcat gaggattggt ccggagccca ccacagactc cttcatcgcc 3121 gtgatgtatg gagagactga gggcagcacc ccagggaatg ctttagtcgt ggaccccaaa 3181 aagccgttta gaaagctcag tcgctttgga aacgctttcc tgaatcgatt catgtgctca 3241 cagctcccca atcaggtcct gaagagcatc agcgtcatcg acagccccgg catcctttct 3301 ggggagaagc agcgcatcag ccgaggctat gacttctgcc aggtcctgca gtggtttgcc 3361 gagagggtgg acaggatcat cctgctcttt gacgctcaca agctggacat ctcagatgaa 3421 ttctcagagg ccatcaaggc cttccggggc caggacgaca agatccgtgt cgtgctgaac 3481 aaggccgacc aagtggacac gcagcagctg atgcgggtct acggggccct catgtggtcc 3541 ctaggcaagg tcatcaacac gcccgaggta ctgcgcgtct acattggctc cttctgggcg 3601 cagcccctgc agaacacgga caaccgccgg ctcttcgagg ctgaggccca ggacctcttt 3661 agagacatcc agagcctccc ccagaaggca gcggtgcgca agctcaacga cctcatcaag 3721 cgagcgaggc tggccaaggt gcatgcctac atcatcagct acctgaagaa ggagatgcca 3781 agtgtatttg gaaaggaaaa caagaagaga gagcttatca gcaggctacc ggaaatctac 3841 attcagctac agcgagaata ccagatttct gcaggggact tccctgaggt caaggctatg 3901 caggaacagc ttgagaacta tgacttcacc aaattccact cgctgaagcc caagctgatc 3961 gaggcagtgg acaacatgct gagcaacaag atctcgcccc tcatgaacct catcagccag 4021 gaggagacga gcacgcccac gcagctggtg cagggcggcg ccttcgatgg caccaccgag 4081 ggccccttca accagggcta cggggagggt gccaaggagg gcgccgacga ggaggagtgg 4141 gtcgtggcca aagacaagcc cgtctacgac gagctcttct acactctgtc gcccatcaat 4201 ggcaagatat caggtgtcaa cgccaagaag gagatggtga cctccaagct gcccaacagc 4261 gtcctgggca agatctggaa gctggccgac tgcgactgcg acggcatgct tgatgaggag 4321 gagttcgcgc tggccaagca cctcatcaag atcaagctcg acggctacga gctgcccagc 4381 agcctgcccc cccacctcgt gcccccctcg cacaggaagt ccctgcccaa ggccgactga 4441 gcggccgcgg atcccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct 4501 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg 4561 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt 4621 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg 4681 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac 4741 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg 4801 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctcacct 4861 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg 4921 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg 4981 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggtgagca 5041 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg 5101 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 5161 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg 5221 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca 5281 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 5341 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca 5401 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga 5461 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg 5521 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga 5581 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt 5641 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg 5701 gccgccactc caccggcggc atggacgagc tgtacaagtg aacgcgtctg gaacaatcaa 5761 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 5821 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 5881 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5941 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 6001 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 6061 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 6121 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 6181 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 6241 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 6301 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca 6361 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg 6421 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg 6481 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 6541 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga 6601 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca 6661 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag 6721 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg 6781 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg 6841 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg 6901 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag 6961 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac 7021 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 7081 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 7141 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 7201 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga 7261 cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 7321 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 7381 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 7441 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 7501 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 7561 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 7621 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 7681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 8041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 8101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 8161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 8221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 8281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 8341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 8401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 8461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 8521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 8581 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 8641 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 8701 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 8761 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 8821 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 8881 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 8941 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 9001 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 9061 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 9121 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 9181 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 9241 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 9301 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 9361 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 9421 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga 9481 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 9541 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 9601 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc 9661 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga 9721 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt 9781 agtagt //