LOCUS       V024224                11422 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024224
VERSION     V024224
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        1960..2171
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             2184..2452
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             2498..5539
                     /label="Parp1(NM_007415)"
                     /note="Parp1(NM_007415)"
                     /gene="Parp1"
     CDS             5546..5569
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        5581..6080
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6125..6787
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6857..6910
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6911..7510
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7517..8105
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8179..8412
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8484..8605
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8624..8759
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8797..8813)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8821..8837
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8845..8875)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8890..8911
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9199..9787)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9958..10818)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10819..10923)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11397..11413
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gcggaggcct cggagaggct
     2521 ttatcgagtg gagtacgcga agagcgggcg cgcctcttgc aagaaatgca gcgagagtat
     2581 tcccaaggac tccctccgca tggccatcat ggtgcagtca cccatgttcg atgggaaagt
     2641 cccacactgg taccacttct cctgcttctg gaaggtgggc cactccatcc ggcagcctga
     2701 tgttgaggtg gatggcttct ctgagctgcg ctgggatgat cagcagaagg tcaagaagac
     2761 ggccgaggct ggaggcgtgg caggcaaagg ccaggatgga agtggcggca aggcggagaa
     2821 gacattgggt gactttttag cggagtacgc caagtccaac aggagcatgt gcaagggctg
     2881 cctggagaag atagagaagg gccagatgcg cctgtccaag aagatggtgg atccagagaa
     2941 gccacagctg ggtatgattg accgctggta ccatccaact tgctttgtca agaagcggga
     3001 cgagctgggc ttccggcctg agtacagtgc cagtcagctc aagggcttta gcctcctctc
     3061 tgcagaagac aaagaagctc tgaagaagca gctcccggcc atcaagaatg aaggaaagag
     3121 aaaaggtgac gaggtggatg gaacagatga agtggccaaa aagaaatcta agaaagggaa
     3181 ggacaaggat agtagtaagc tggagaaggc cctcaaggct cagaatgagc tgatctggaa
     3241 tatcaaagac gagctgaaga aagcgtgttc caccaacgac ctgaaggagc tgctcatctt
     3301 caaccagcag caggtgccgt caggagagtc agcgatcttg gacagagttg ctgacggcat
     3361 ggcgtttggg gcccttctgc cctgcaagga gtgttcaggc cagctggtct ttaagagcga
     3421 cgcttattac tgtactgggg atgtcactgc ctggaccaag tgcatggtca agacacagaa
     3481 tcctagccga aaggaatggg taactccaaa ggaattccga gaaatatcct acctcaagaa
     3541 gttaaaggtc aaaaaacagg accgaatatt ccctccagaa agcagcgccc cagcaccact
     3601 ggcactgccc ctctctgtca cctcagcacc cacagctgtg aactcctctg ctccagcaga
     3661 caagcccctg tctaacatga agatcctgac tcttgggaag ctctcccaga acaaggacga
     3721 agcaaaagct gtgattgaga aactcggagg caagttgaca ggatctgcca acaaggcctc
     3781 cttgtgtatc agcactaaaa aggaggtgga gaagatgagt aagaagatgg aggaagtgaa
     3841 agcggccaac gttcgagttg tgtgtgagga cttcctccag gacgtgtctg cctccactaa
     3901 aagcctccaa gagctgctct cggcccacag cttgtcctcg tggggggctg aggtgaaggc
     3961 agagcctggt gaagtggtgg cccccaaggg gaagtcagct gcaccctcca agaagagcaa
     4021 gggtgctgtc aaggaggaag gtgtcaacaa atctgaaaag aggatgaaat taactctgaa
     4081 gggaggagca gccgttgatc ctgactctgg tctggaacac tctgcacacg tcctggagaa
     4141 aggtgggaag gtgttcagcg ccacacttgg cctggtggac attgtgaaag ggacgaactc
     4201 ctattacaaa ctgcagcttc tggaggacga caaggagagc aggtactgga tcttccggtc
     4261 ctggggccgg gtgggcacag ttatcggcag taacaaactt gagcagatgc cctccaaaga
     4321 ggacgctgtt gagcacttca tgaagctgta tgaagagaag actgggaatg cctggcactc
     4381 gaaaaacttc acaaagtatc ccaagaagtt ctaccctctg gagattgact atggccagga
     4441 cgaagaggca gtaaagaagc tgacggtgaa gcctggcacc aagtcgaagc tgccgaagcc
     4501 agtgcaggag ctcgtgggga tgatcttcga cgtggagagc atgaaaaagg ccttggtgga
     4561 gtacgagatt gacctccaga agatgccctt ggggaagctg agcagaaggc agatccaggc
     4621 cgcctactct atcctcagcg aggtccagca ggcagtgtct caaggcagca gtgaatccca
     4681 gatcctagat ctctccaatc gcttctacac tctgatcccc catgactttg gaatgaagaa
     4741 gcccccactc ctgaacaacg cagacagcgt gcaggccaag gtggagatgc tagacaacct
     4801 cctggacatc gaggtggcct atagtcttct caggggtggc tctgacgaca gcagcaagga
     4861 tcccatcgac gtcaactacg agaaactcaa aactgacatt aaggtggttg acagagattc
     4921 tgaagaggcc gaggtcatca ggaagtacgt gaagaacact catgctacca cgcacaacgc
     4981 ctatgacctg gaagtgatcg atatcttcaa gatagagcgc gagggggaga gccagcgcta
     5041 caagcccttc aggcagcttc acaaccggag gctgctgtgg cacggctcca ggaccaccaa
     5101 ctttgctggc atcctgtcgc agggtctgcg gatagcccca cctgaagcgc ctgtgacagg
     5161 ctacatgttt gggaaaggga tctactttgc cgacatggtg tccaaaagtg caaactactg
     5221 ccacacatct cagggagacc cgattggctt aatactgctg ggagaggttg cccttggaaa
     5281 catgtatgaa ctcaagcatg cttcacatat cagcaagtta cccaagggca agcacagtgt
     5341 caaaggtttg ggaaaaacca cccctgaccc ttcggccagc atcaccctgg agggtgtaga
     5401 ggttccactg ggaacaggga tcccatctgg tgtcaacgac acctgcctgc tgtataatga
     5461 gtacattgtc tacgacattg ctcaggtgaa tctcaaatac ctgctgaaac tcaagttcaa
     5521 ttttaagaca tccctgtggg gatccgacta caaggacgac gatgacaagt agcggccgct
     5581 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     5641 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     5701 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     5761 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     5821 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     5881 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     5941 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     6001 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     6061 cctcatctcc gggcctttcg acctgcagcc caagcttacc atggagagcg acgagagcgg
     6121 cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga
     6181 gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag
     6241 caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt
     6301 ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa
     6361 cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag
     6421 cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg
     6481 cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga
     6541 gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct
     6601 gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat
     6661 ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct
     6721 gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc
     6781 cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg
     6841 ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     6901 tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6961 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7021 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7081 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7141 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7201 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7261 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7321 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7381 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7441 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7501 cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7561 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7621 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7681 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7741 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7801 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7861 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7921 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7981 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8041 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8101 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8161 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt
     8221 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8281 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8341 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8401 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8461 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8521 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8581 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8641 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8701 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8761 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     8821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8941 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9001 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9061 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9601 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10921 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10981 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    11041 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    11101 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    11161 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    11221 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    11281 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    11341 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    11401 aacgacggcc agtgccaagc tg
//
