LOCUS       V030532                11615 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030532
VERSION     V030532
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        1960..2171
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             2184..2452
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             2498..5755
                     /label="Kdm4b(NM_172132)"
                     /note="Kdm4b(NM_172132)"
                     /gene="Kdm4b"
     promoter        5774..6273
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6318..6980
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7050..7103
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7104..7703
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7710..8298
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8372..8605
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8677..8798
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8817..8952
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8990..9006)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9014..9030
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9038..9068)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9083..9104
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9392..9980)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10151..11011)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11012..11116)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11590..11606
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gggtccgagg accacagcgc
     2521 ccagaacccc agctgtaaga tcatgacttt ccgccccacc atggatgaat tcagggactt
     2581 caacagatat gtggcgtaca ttgagtcgca gggtgcccac cgtgctggcc tggccaagat
     2641 cattccaccc aaggagtgga agccacggca gacgtatgat gacatcgacg atgtggtcat
     2701 cccagcgcct attcagcagg tggtgacggg ccagtcgggg cttttcacac agtacaacat
     2761 ccagaagaag gccatgactg tgggggagta ccgccgccta gccaatagtg aaaagtactg
     2821 taccccacgc catcaggact tcgatgactt ggagcgcaag tactggaaga acctgacctt
     2881 tgtctcaccc atctatggag ctgacatcag tggctccctg tacgacgatg acgtggcgca
     2941 gtggaacatc ggcaatctga ggaccatcct agacatggtg gagcgcgaat gcgggaccat
     3001 cattgagggc gtgaatacac cctacttgta ctttggcatg tggaagacca cgtttgcctg
     3061 gcacacagag gacatggacc tctacagcat caactacctg cacttcggcg agcctaagtc
     3121 ctggtacgcc ataccaccgg agcacggcaa gcgcctggag cgcctggcca taggcttctt
     3181 ccctgggagc tcacagggct gtgatgcctt cctaaggcac aagatgacac tcatctcgcc
     3241 catcatcctg aagaagtacg gcatcccctt cagccggatt acacaggaag ctggggagtt
     3301 catgatcacg ttcccctatg gttaccatgc gggcttcaac catggcttta actgcgctga
     3361 gtccactaac ttcgccacac tgcgctggat cgactatggc aaagtggcca cacagtgtac
     3421 atgtcgaaag gacatggtca agatctccat ggacgtgttt gtacgcatcc tgcagcccga
     3481 acgctatgag caatggaagc aaggccgtga cctcacagtg ctggaccaca cgaggcccac
     3541 agccctgagc agtcctgagc tcagctcctg gagtgcctcg cgcacctcca tcaaggccaa
     3601 gctcctgcgc agacaaatta gtgtgaagga gagcagaccc tggagaaagg ctgaggagga
     3661 gaggaggcgg gagccgacca ggaggccagg gccggcgtca caccggagac ggagccagcc
     3721 caagaagtca aaacctgaag agtccaggtc tccaggggaa gccacggctg gggtatctac
     3781 tctggatgag gccaggggct gctcccgagg agaggccatg cctgaggacg aggaggagga
     3841 ggagctgcta ccttcacagg gccatgaggc cgagggtgtg gaagaggatg gcagaggcaa
     3901 gcctcggcct actaaggcca gaaataagaa gaagactccc agcccgtcgt cacccccgtt
     3961 gctgtctgcc ccaccagccc tattccccac cgaggaggtc ctgaggccac caccccaacc
     4021 caagtctcca gggccagcta tgggccccat ggctgcagag gggggtcctc caccaacacc
     4081 cctcaatgtc gtgcctcctg gggcgccagt tgaggaagca gaggtgcggc cacggcccat
     4141 tatcccaatg ctgtacgtgt tgccccgcac cagcagcacc gatggggaca gggagcactc
     4201 tgcccacgca cagttggcac ccatggagct ggggccagaa gaggaaaacc aggcccaggc
     4261 tggtgactca cagggaacaa cccctttctc taagctgaag gtggagatca aaaagagccg
     4321 gcgtcacccc ttgggtcggc cacccactcg atccccgctg tctgtggtca agcaggaggc
     4381 ctcaagtgat gaagaagcct tcctgttctc aggagaggat gatgtcactg atcctgaggc
     4441 cttgagatcg ctgctgtccc tgcagtggaa gaacaaggcg gccagcttcc aagccgagag
     4501 gaagttcaat gcggcagccg ccctcagtga gccctactgt gccatctgta ccctcttcta
     4561 cccctacagc cagtcagtac agacagagcg ggacagtgct gtccagccgc cctccaaaag
     4621 tggccagagg acacggccac tgatccctga gatgtgcttc acttcaagtg gggagaacac
     4681 agagccgctg cctgctaact cctacgtcgg tgaggacggc accagtccac tcatctcctg
     4741 tgcccactgc tgcctgcagg tccatgccag ttgctatggt gtccggcctg agttggccaa
     4801 agagggctgg acatgttccc ggtgtgcggc ccacgcttgg actgcggaat gctgtttgtg
     4861 caacctgcgg gggggagcgc tacagaggac cacagagcac aggtggattc acgtgatctg
     4921 cgccattgca gtccctgagg tacgattcct caatgtgatt gaacgcaacc cagtggacgt
     4981 cagtgccatc cctgagcagc gatggaaact gaaatgcatt tactgccgca agcgtatgaa
     5041 gagagtgtcg ggtgcctgca tccagtgctc ctatgagcac tgctccacat ccttccatgt
     5101 gacctgtgcg catgctgctg gtgtcctcat ggaacccgat gactggccct atgtggtgtc
     5161 catcacctgc ctcaagcacc gggccagcgg agcagggggc cagctcctgc gcacagtgtc
     5221 cctgggccag atcgtcatca ccaagaaccg caatgggctc tactatcgct gccgggtcat
     5281 tggcaccact gcgcagacat tctacgaggt caactttgac gatggctcct acagtgacaa
     5341 cctgtaccca gagagcatca cgagcagaga ctgcctacgg ctagggccac ctcctgaggg
     5401 cgagctggtg gagcttcggt ggacagacgg taatctgtac agagccaggt ttatctccat
     5461 ggccaccagc ctcatctacc aggtagagtt tgaggatggg tctcagctga ctgtgaagcg
     5521 tggggacatc ttcacactgg aagaggagct gcccaagagg gtgcgttccc ggctgtcact
     5581 gagcactggc acaccacagg agcccagctt ctctggagac gatgtgaagg ctgccaagcg
     5641 cccacgggtg gcctcagtgc tggccaccac cactgaggac acagggcgta gcccagaata
     5701 cctgtccttc atggagagcc tgctgcaggc acagggccgg cctggagcac ccttctaggg
     5761 atccgcggcc gctgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     5821 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     5881 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     5941 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     6001 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     6061 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     6121 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     6181 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     6241 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatggaga
     6301 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg
     6361 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca
     6421 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga
     6481 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc
     6541 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg
     6601 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg
     6661 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca
     6721 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc
     6781 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact
     6841 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc
     6901 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca
     6961 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca
     7021 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg
     7081 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     7141 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     7201 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     7261 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     7321 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     7381 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     7441 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     7501 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     7561 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     7621 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     7681 tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa
     7741 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7801 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7861 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7921 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7981 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8041 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8101 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8161 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8221 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8281 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     8341 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     8401 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     8461 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8521 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8581 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8641 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8701 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8761 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8821 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8881 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8941 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     9001 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9061 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9121 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9181 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9241 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9301 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9361 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9421 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9481 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9541 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9601 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9661 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9721 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9781 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9841 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9901 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9961 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10021 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10081 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10141 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10201 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10261 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10321 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10381 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10441 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10501 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10561 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10621 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10681 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10741 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10801 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10861 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10921 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10981 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11041 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11101 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    11161 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    11221 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    11281 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    11341 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    11401 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    11461 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    11521 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    11581 cacgacgttg taaaacgacg gccagtgcca agctg
//
