LOCUS       V032419                10574 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032419
VERSION     V032419
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        1960..2171
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             2184..2452
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             2498..4720
                     /label="Atg7(NM_001253717)"
                     /note="Atg7(NM_001253717)"
                     /gene="Atg7"
     promoter        4733..5232
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5277..5939
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6009..6062
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6063..6662
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6669..7257
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7331..7564
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7636..7757
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7776..7911
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7949..7965)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7973..7989
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7997..8027)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8042..8063
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8351..8939)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9110..9970)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9971..10075)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10549..10565
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg catacaggcc tctggaaaat
     2521 tcccacgagc acagtggtga ggccaactcc acactgcttt cctgccatga ggctttcctc
     2581 atctggatgt gagcacctga cagtgaggct ggtaagaaca gtagccatgg gggaccctgg
     2641 actggccaag ttgcagttcg ccccctttaa tagtgccctg gacgttggct tctggcacga
     2701 actgacccag aagaagttga acgagtaccg cctggacgag gcacccaaag acatcaaggg
     2761 ctattactac aatggtgact ctgctggtct gcccacccgc ttgacgttgg agttcagtgc
     2821 ttttgacatg agtgcctcca cgcctgccca ctgctgcccg gccatgggaa ccctgcacaa
     2881 caccaacaca cttgaggctt ttaagacagc agacaagaag ctccttctgg agcagtcagc
     2941 aaatgagatc tgggaagcca taaagtcagg tgctgctctc gaaaacccca tgctcctcaa
     3001 caagtttctg ctcctgacct tcgcggacct aaagaagtac cacttctact actggttttg
     3061 ctgccccgcc ctctgtcttc ctgagagcat ccctctaatc cggggacctg tgagcttgga
     3121 tcaaaggctt tcaccaaaac agatccaggc cctggagcat gcctatgatg atctgtgtcg
     3181 agccgaaggc gtcacggccc tgccctactt cttattcaag tacgatgacg acactgttct
     3241 ggtctccttg ctcaaacact acagtgattt cttccaaggt caaaggacaa agataacagt
     3301 tggtgtgtac gatccctgta acctagccca gtaccctgga tggcctttga ggaatttttt
     3361 ggtcctggca gcccacagat ggagcggcag tttccagtcc gttgaagtcc tctgctttcg
     3421 ggaccgcacc atgcagggag ctagagacgt gacacatagc atcatctttg aagtgaaact
     3481 tccagaaatg gcatttagcc cagattgtcc taaagctgtt ggctgggaga agaaccagaa
     3541 aggaggcatg ggtccgagga tggtgaacct cagtggatgt atggacccca aaaggctggc
     3601 tgagtcatct gtggatctga atctcaagct gatgtgctgg cgattggtcc ccaccttgga
     3661 cttggacaag gtcgtgtctg tcaagtgcct gctgctggga gctggtacct tggggtgtaa
     3721 tgtggctagg acactgatgg gctggggcgt cagacatgtc acctttgtgg ataacgccaa
     3781 gatctcctac tccaatcccg tgaggcagcc tctgtatgaa tttgaagatt gtctaggggg
     3841 tggcaagccc aaggccctgg ctgcagcaga gcggctacag aaaatatttc ccggagtgaa
     3901 tgccagaggg ttcaacatga gcatccccat gccaggacac cctgtgaact tctctgacgt
     3961 cacgatggag caggcccgca gagatgtgga gcagctggag cagctcattg ataaccatga
     4021 tgtcatcttc ctgctaatgg acaccaggga gagccggtgg cttcctactg ttattgcagc
     4081 cagcaagcga aagctggtca tcaacgctgc cttggggttt gatacctttg ttgtcatgag
     4141 acatggcctg aagaaaccca agcagcaggg agccggagac ctctgcccaa gccatcttgt
     4201 agcacctgct gacctgggct cctcactttt tgccaacatc cctggataca agcttggctg
     4261 ctacttctgc aatgatgtgg tggctccagg agattcaacc agagaccgga ctctggacca
     4321 gcagtgcaca gtgagccgcc caggcctggc cgtgattgca ggtgccctgg ctgtggagct
     4381 gatggtctct gtcctgcagc atcctgaggg gggctacgcc atcgccagca gcagtgatga
     4441 ccgcatgaat gagcctccca cctcgctggg acttgtgcct caccagatcc ggggttttct
     4501 gtcacggttc gataatgttc ttcctgtcag cctggcattt gataaatgta cagcctgttc
     4561 acccaaagtt cttgatcagt acgagcgaga aggattcacc ttcctagcga aggtttttaa
     4621 ctcctcacat tccttcttag aagacttgac cggtcttacc ctgctccatc aagagaccca
     4681 agctgctgag atctgggaca tgagtgacga ggagactgtc tgagcggccg ctgggtaggg
     4741 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     4801 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     4861 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     4921 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     4981 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     5041 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     5101 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     5161 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     5221 ccgggccttt cgacctgcag cccaagctta ccatggagag cgacgagagc ggcctgcccg
     5281 ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg
     5341 gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag
     5401 gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact
     5461 tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct
     5521 acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct
     5581 accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg
     5641 aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc
     5701 accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg
     5761 gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca
     5821 gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca
     5881 acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca
     5941 gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg
     6001 gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     6061 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     6121 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     6181 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     6241 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     6301 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     6361 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     6421 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     6481 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6541 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6601 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     6661 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6721 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6781 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6841 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6901 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6961 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7021 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7081 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7141 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7201 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7261 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7321 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     7381 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7441 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7501 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7561 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7621 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7681 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7741 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     7801 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7861 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     7921 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7981 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8041 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8101 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8161 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8221 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8281 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8341 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8401 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8461 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8521 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8581 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8641 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8701 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8761 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8821 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8881 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8941 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9001 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9061 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10081 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10141 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10201 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10261 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10321 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10381 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    10441 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10501 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10561 ccagtgccaa gctg
//
