LOCUS       V026465                11005 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026465
VERSION     V026465
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5031
                     /label="Zfyve28(NM_001015039)"
                     /note="Zfyve28(NM_001015039)"
                     /gene="Zfyve28"
     CDS             5038..5103
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5147..5358
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5371..5639
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5668..5677
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5698..6360
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6430..6483
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6484..7083
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7084..7672
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7746..7979
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8051..8172
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8212..8347
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8380..8396)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8404..8420
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8428..8458)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8473..8494
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8782..9370)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9541..10401)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10402..10506)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10980..10996
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga tgaacagatt ccgaaagtgg
     2341 ctgtacaaac ccaagaggtc agacccacaa ctgctggccc agttctacta tgctgatgag
     2401 gagctgaacc aggtggctgc agagctggac agcctggatg ggcggaagga gccccagagg
     2461 tgcacactgc ttgtcagcca gttccgctcc tgtcaggaca atgtattaaa catcattaat
     2521 cagatcatgg aggagtgtat ccctcaggac cgtgcccctc gggatttctg cgtcaagttt
     2581 ccagaggaga tccggcatga caacttggct ggccagctgt ggttcggggc tgagtgcctg
     2641 gcagctggct ccatcatcat gaatcgggag ctggagagca tggccatgcg gccactggcc
     2701 aaggagctga cacgcagcct ggaggacgtg cggggcactc tccgtgacca ggcactccga
     2761 gacctcaaca cttacacaga gaagatgcgg gaggcactga ggcgctttga tgtcctgttt
     2821 gctgagtttg aactgagcta tgtctcagcc atggtgccag tgaagtcccc cagggagtac
     2881 tatgtgcagc aggaggtcat cgtgctgttc tgtgagacag tggagagagc cttggatttt
     2941 gggtatctga cccaggacat gatcgacgac tacgaacccg ccctcatgtt caccattcct
     3001 cggctggcca ttgtgtgtgg ccttgtggtc tatgcagatg gacccctgaa cttggaccgg
     3061 aaggtggagg acatgtctga gctgttccgg cccttccaca cgttgctgcg gaaaataagg
     3121 gatctgctgc aggcgctgac ggaggaggag ctgcacacac tggagcggag cctctgtgtt
     3181 tctcaggatg tggagctccc catccgggca gacacccagg cacccagtgc cctggcaccc
     3241 accttttctg catctcttcc tcccgaggag acactttcag ccagtgccaa caacccagag
     3301 gcagaactgg cctgctcaat gcagtatgat gatcaggagt tggaggaact cagccgcatg
     3361 gtccacaggg ctggggatga gatgtcttct ctgctttcac cacccagtgc ctgccagtcc
     3421 ccggcacata ggccaggctc agaggctagc cctcgagggg aggcctctcc agccagagcc
     3481 cggctgaaat caggcagtga tgaggaggaa agggtgttct tcatggatga tgtggaggtg
     3541 acagagtctc ctgccaggcc tgaatcccca gggaacacat ttgagttgac gcagggtaat
     3601 gcccagcaga gaggacaaga tggccagagt ggagaggtgg gggtcgaggc acctgccctg
     3661 gtaaaggagg aagactggag caacaataat gttgagggtg acaagataaa gctggccagc
     3721 ctcatgggct ccacttcctg cagctgccta gactcacagc tctacctgga cggctgggag
     3781 gtgagtgcag aggatgctga gactgcagag atgattgcac accggaccgg gggaatgaaa
     3841 ctgtcagcca cagtcatctt caaccccaag tctcccactt cccaggactc agctgtggca
     3901 gcccaagaag ccccaggcca tggtacttcc cctttggagc ccagggcaga ggggacaggg
     3961 gacaattcac acaaactcag cactacagcc accaactgcc tcctccattc ctgtgtgtgc
     4021 tgtgggagct gtggggacag cagggacgat gctgtagagc gcctgcggga gaagtgtggc
     4081 ccaggaagtg tcatcagtgc ctctaaccct tctgtaagct tagccaaggg tggtgacaaa
     4141 gagcccgaga gaatagatga ggcccagcct tctgatgtca ccctgcctgc agaagatgcc
     4201 tccaacaggc aggagcccaa agcccctgct tccagcaagt gcctggcaca cacctcaggg
     4261 cctcaggtgg acacagcgag caggttgcaa ggagagggtg aggtcaaagg ccagccagag
     4321 ccagaggcca gaaagcagga ccctgagaag tcccctgtgg tcagcgggga cagtccaaga
     4381 ggggatgtgg cccagacaga acaccagcac ctgttgggct ccagctccac ggttggttcc
     4441 tgctccttag acaacaccag gttggatgtg gccactgctg ccatgcctgt cacccccatg
     4501 gccaccagag agaagatccg gtccaggttc catggcagcc atgacctgat ccaccgcctg
     4561 tttgtctgca tttcaggtgt agcagaccag ctacagacga actatgctag cgacctgaga
     4621 agtattctca aaacactgtt tgaggtcatg gccaccaagc cagaaacaga tgacaaagag
     4681 aagctaaaga aggtaactca gaccctgcgg agtgcagcct tggaggactg tgcactgtgc
     4741 caggagactg tgtcatcctc agaactggca gccaagaccc gagatgggga ctttgaagac
     4801 cctcctgagt gggtgccaga tgaggcctgt ggcttctgca cctcctgtaa agctcccttc
     4861 accgtcatcc gcaggaagca ccactgtcgc agctgtggta agatcttctg ttcccgctgc
     4921 tcatcccact cggcgccgct tccgcgctac ggacaggtga agcccgtccg tgtgtgtaca
     4981 cactgctaca tgttccacgt cacacccttc tacagtgaca agacgggcat gggatccgac
     5041 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     5101 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     5161 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     5221 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5281 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5341 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     5401 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     5461 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     5521 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     5581 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     5641 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     5701 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     5761 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     5821 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     5881 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     5941 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     6001 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     6061 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     6121 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     6181 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     6241 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     6301 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     6361 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     6421 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     6481 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6541 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6601 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6661 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6721 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6781 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6841 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6901 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6961 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7021 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7081 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7141 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7201 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7261 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7321 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7381 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7441 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7501 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7561 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7621 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     7681 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7741 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     7801 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7861 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7921 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7981 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8041 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8101 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8161 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     8221 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8281 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8341 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     8401 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8461 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8521 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8581 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8641 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8701 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8761 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9181 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     9241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9301 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9421 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9481 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9541 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9601 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9661 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9721 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9781 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9841 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9901 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9961 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10021 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10081 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10141 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10201 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10261 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10321 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10381 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10441 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10501 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10561 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    10621 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10681 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10741 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10801 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    10861 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10921 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10981 taaaacgacg gccagtgcca agctg
//
