LOCUS       V044498                10186 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044498
VERSION     V044498
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4212
                     /label="ZDHHC17(NM_015336)"
                     /note="ZDHHC17(NM_015336)"
                     /gene="ZDHHC17"
     CDS             4219..4284
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4328..4539
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4552..4820
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4849..4858
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4879..5541
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5611..5664
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5665..6264
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6265..6853
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6927..7160
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7232..7353
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7393..7528
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7561..7577)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7585..7601
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7609..7639)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7654..7675
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7963..8551)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8722..9582)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9583..9687)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10161..10177
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc agcgggagga gggatttaac
     2341 accaagatgg cggacggccc ggatgagtac gataccgaag cgggctgtgt gccccttctc
     2401 cacccagagg aaatcaaacc ccaaagccat tataaccatg gatatggtga acctcttgga
     2461 cggaaaactc atattgatga ttacagcaca tgggacatag tcaaggctac acaatatgga
     2521 atatatgaac gctgtcgaga attggtggaa gcaggttatg atgtacggca accggacaaa
     2581 gaaaatgtta ccctcctcca ttgggctgcc atcaataaca gaatagattt agtcaaatac
     2641 tatatttcga aaggtgctat tgtggatcaa cttggagggg acctgaattc aactccattg
     2701 cactgggcca caagacaagg ccatctatcc atggttgtgc aactaatgaa atatggtgca
     2761 gatccttcat taattgatgg agaaggatgt agctgtattc atctggctgc tcagttcgga
     2821 catacctcaa ttgttgctta tctcatagca aaaggacagg atgtagatat gatggatcag
     2881 aatggaatga cgcctttaat gtgggcagca tatagaacac atagtgtgga tccaactaga
     2941 ttgcttttaa cattcaatgt ttcagttaac cttggtgaca agtatcacaa aaacactgct
     3001 ctgcattggg cagtgctagc agggaatacc acagtcatta gccttcttct ggaagctgga
     3061 gctaatgttg atgcccagaa tatcaagggc gaatcagcgc ttgatttggc aaaacagaga
     3121 aaaaatgtgt ggatgatcaa ccacttacaa gaggcaaggc aagcaaaagg atatgacaat
     3181 ccgtccttcc ttagaaagct gaaagctgat aaggaatttc ggcagaaagt aatgttagga
     3241 actcctttcc tagttatttg gctggttggg tttatagcag acctaaatat tgattcttgg
     3301 ctcattaaag ggctaatgta tggtggtgtt tgggctacag tacagtttct ttcaaaatcc
     3361 tttttcgatc attcaatgca tagtgcattg ccccttggga tatatttggc aaccaaattc
     3421 tggatgtatg tgacgtggtt cttctggttt tggaatgatc tcaacttttt atttatccat
     3481 cttccattcc ttgccaatag tgttgcactt ttctacaatt ttggaaaatc ttggaaatca
     3541 gatccaggga ttattaaagc aacagaagag caaaagaaaa agacaatagt tgaacttgca
     3601 gagacaggaa gtctggacct cagtatattc tgcagtacct gtttgatacg aaaaccggtg
     3661 aggtccaaac attgtggtgt gtgcaaccgc tgtatagcaa aatttgatca tcattgccca
     3721 tgggtgggta actgtgtagg tgcaggcaac catagatatt ttatgggcta cctattcttc
     3781 ttgcttttta tgatctgctg gatgatttat ggttgtatat cttactgggg actccactgt
     3841 gagaccactt acaccaagga tggattttgg acatacatta ctcagattgc cacgtgttca
     3901 ccttggatgt tttggatgtt cctgaacagt gttttccact tcatgtgggt ggctgtatta
     3961 ctcatgtgtc agatgtacca gatatcatgt ttaggtatta ctacaaatga aagaatgaat
     4021 gccaggagat acaagcactt taaagtcaca acaacgtcta ttgaaagccc attcaaccat
     4081 ggatgtgtaa gaaatattat agacttcttt gaatttcgat gctgtggcct ctttcgtcct
     4141 gttatcgtgg actggaccag gcagtataca atagaatatg accaaatatc aggatctggg
     4201 taccagctgg tgggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     4261 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     4321 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4381 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4441 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4501 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     4561 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     4621 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     4681 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     4741 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     4801 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     4861 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     4921 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     4981 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     5041 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     5101 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     5161 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     5221 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     5281 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     5341 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     5401 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     5461 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     5521 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     5581 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     5641 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     5701 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     5761 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     5821 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     5881 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     5941 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6001 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6061 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6121 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6181 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6241 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     6301 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6361 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6421 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6481 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6541 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6601 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6661 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6721 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6781 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6841 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6901 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     6961 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7021 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7081 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7141 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7201 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7261 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7321 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7381 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7441 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7501 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     7561 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7621 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7681 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7741 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7801 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7861 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7921 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7981 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8041 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8101 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8161 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8221 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8281 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8341 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8401 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8461 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8521 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8581 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8641 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8701 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8761 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8821 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8881 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8941 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9001 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9061 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9121 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9181 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9241 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9301 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9361 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9421 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9481 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9541 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9601 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9661 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9721 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9781 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     9841 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     9901 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     9961 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10021 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10081 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    10141 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
