LOCUS       V032114                11039 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032114
VERSION     V032114
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5871
                     /label="WAPL(NM_015045)"
                     /note="WAPL(NM_015045)"
                     /gene="WAPL"
     CDS             5878..5904
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5908..5934
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5938..5964
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6009..6220
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6233..6501
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6528..7127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7134..7722
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7796..8029
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8101..8222
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8241..8376
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8414..8430)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8438..8454
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8462..8492)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8507..8528
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8816..9404)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9575..10435)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10436..10540)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11014..11030
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgacatcc agatttggga aaacatacag taggaaaggt
     2341 ggaaatggca gttcaaaatt cgatgaagtc ttttccaaca aacggactac ccttagcaca
     2401 aaatggggag agaccacatt tatggctaaa ttagggcaga agaggcccaa tttcaaacca
     2461 gatatccaag aaattccgaa gaaacctaaa gtggaagaag aaagtactgg agatcctttt
     2521 ggatttgata gtgatgatga gtctctacca gtttcttcaa agaatttagc ccaggttaag
     2581 tgttcctctt attcagaatc tagtgaagct gctcagttgg aagaggtcac ttcagtactt
     2641 gaagctaata gcaaaattag tcatgtggtc gttgaagaca ctgtcgtttc tgataaatgc
     2701 ttccctttgg aggacacttt acttgggaaa gaaaagagca caaaccgaat tgtagaagat
     2761 gatgcaagca taagtagctg taataaatta ataacttcag ataaagtgga gaattttcat
     2821 gaagaacatg aaaagaatag tcaccatatt cacaaaaatg ctgatgacag tactaagaaa
     2881 cccaatgcag aaactacagt ggcttctgaa atcaaggaaa caaatgatac ttggaactcc
     2941 cagtttggga aaaggccaga atcaccatca gaaatatctc caatcaaggg atctgttaga
     3001 actggtttgt ttgaatggga taatgatttt gaagatatca gatcagaaga ctgtatttta
     3061 agtttggata gtgatcccct tttggagatg aaggatgacg attttaaaaa tcgattggaa
     3121 aatctgaatg aagccattga ggaagatatt gtacaaagtg ttcttaggcc aaccaactgt
     3181 aggacgtact gtagggccaa taaaacgaaa tcctcccaag gagcatcaaa ttttgataag
     3241 ctgatggacg gcaccagtca ggccttagcc aaagcaaaca gtgaatcgag taaagatggc
     3301 ctgaatcagg caaagaaagg gggtgtaagt tgtgggacca gttttagagg gacagttgga
     3361 cggactagag attacactgt tttacatcca tcttgcttgt cagtttgtaa tgttaccata
     3421 caggatacta tggaacgcag catggatgag ttcactgcat ccactcctgc agatttggga
     3481 gaagctggtc gtctcagaaa aaaggcagat attgcaactt ctaagactac tactagattt
     3541 cgacctagta atactaaatc caaaaaggat gttaaacttg aattttttgg ttttgaagat
     3601 catgagacag gaggtgatga aggaggttct ggaagttcta attacaaaat taagtatttt
     3661 ggctttgatg atctcagtga aagcgaagat gatgaagatg atgactgtca agtagaaaga
     3721 aagacaagca aaaaaagaac taaaacagct ccatcaccct ccttgcagcc tcccccagaa
     3781 agcaatgata attcccagga cagtcagtct ggtactaaca atgcagaaaa cttggatttt
     3841 acagaggact tgcctggtgt gcctgaaagt gtgaagaagc ccataaataa acaaggagat
     3901 aaatcaaagg aaaataccag aaagattttt agtggcccca aacggtcacc cacaaaagct
     3961 gtatataatg ccagacattg gaatcatcca gattcagaag aactgcctgg gccaccagta
     4021 gtaaaacctc agagtgtcac agtgaggctg tcttcaaagg aaccaaatca aaaagatgat
     4081 ggagttttta aggctcctgc accaccatcc aaagtgataa aaactgtgac aatacctact
     4141 cagccctacc aagatatagt tactgcactg aaatgcagac gagaagacaa agaattatat
     4201 actgttgttc agcacgtgaa gcacttcaac gatgttgtag aatttggtga aaatcaagag
     4261 ttcactgatg acattgagta cttgttaagt ggcttaaaga gcactcagcc tctaaacaca
     4321 cgttgcctta gtgttattag cttggctact aaatgtgcca tgcccagttt tcgaatgcac
     4381 ctgagagcac atgggatggt agcaatggtc tttaaaacct tggatgattc ccagcaccat
     4441 cagaatctgt ccctctgtac agctgccctc atgtatatac tgagtagaga tcgtttgaac
     4501 atggatcttg atagagctag cttagatcta atgattcgac ttttggaact ggaacaagat
     4561 gcttcatcag ccaagctact gaatgaaaaa gacatgaaca aaattaaaga aaaaatccga
     4621 aggctctgtg aaactgtaca caacaagcat cttgatctag aaaatataac gactgggcat
     4681 ttagctatgg agacattatt atcccttact tctaaacgag caggagactg gtttaaagaa
     4741 gaactccggc ttttgggtgg tctggatcat attgtagata aagtaaaaga atgtgtggat
     4801 catttaagta gagatgagga tgaagagaaa ctggtagcct cactatgggg agcagagaga
     4861 tgtttacgag ttttagaaag tgtaactgtg cataatcccg aaaatcaaag ctacttgata
     4921 gcatataaag attcccaact tattgtttca tcagctaaag cattacagca ttgtgaagaa
     4981 ctgattcagc agtacaaccg tgctgaggac agcatatgct tagctgacag taagcctctg
     5041 cctcaccaga atgtaactaa ccatgtaggc aaagcagtgg aggactgcat gagggccatc
     5101 atcggggtgt tgcttaattt aactaatgat aatgagtggg gcagcaccaa aacaggagag
     5161 caggacggtc tcataggcac agcgctgaac tgtgtgcttc aggttccaaa gtacctacct
     5221 caggagcaga gatttgatat tcgagtgctg ggcttaggtc tgctgataaa tctagtggag
     5281 tatagtgctc ggaatcggca ctgtcttgtc aacatggaaa catcgtgctc ttttgattct
     5341 tccatctgta gtggagaagg ggatgatagt ttaaggatag gtggacaagt tcatgctgtc
     5401 caggctttag tgcagctatt ccttgagcga gagcgggcag cccagctagc agaaagtaaa
     5461 acagatgagt tgatcaaaga tgctcccacc actcagcatg ataagagtgg agagtggcaa
     5521 gaaacaagtg gagaaataca gtgggtgtca actgaaaaga ctgatggtac agaagagaaa
     5581 cataagaagg aggaggagga tgaagaactt gacctcaata aagcccttca gcatgccggc
     5641 aaacacatgg aggattgcat tgtggcctcc tacacagcac tacttcttgg gtgtctctgc
     5701 caggaaagtc caatcaatgt aaccactgtg cgggaatatc tgccagaagg agacttttca
     5761 ataatgacag agatgctcaa aaaatttttg agttttatga atctcacttg tgctgttgga
     5821 acaactggcc agaaatctat ctctagagtg attgaatatt tggaacattg cggatcctac
     5881 ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta tgctagctat
     5941 ccttatgatg tcccagacta cgcataggcg gccgcgaagg atctgcgatc gctccggtgc
     6001 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     6061 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     6121 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     6181 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg
     6241 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
     6301 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
     6361 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
     6421 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
     6481 tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagatg accgagtaca
     6541 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     6601 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     6661 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6721 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6781 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6841 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6901 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6961 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7021 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7081 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac
     7141 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7201 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7261 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7321 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7381 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7441 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7501 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7561 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7621 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7681 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca
     7741 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7801 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
     7861 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7921 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7981 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8041 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8101 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8161 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8221 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
     8281 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8341 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
     8401 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8461 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8521 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8581 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8641 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8701 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8761 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8821 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8881 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8941 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9001 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9061 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9241 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10561 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    10621 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    10681 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    10741 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    10801 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    10861 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    10921 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    10981 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
