LOCUS V019256 13622 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019256 VERSION V019256 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2005..2208 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2297..7732 /label="VIRMA(NM_015496)" /note="VIRMA(NM_015496)" /gene="VIRMA" promoter 7781..7992 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 8005..8273 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 8302..8311 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 8332..8994 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 9064..9117 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 9118..9717 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 9718..10305 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 10379..10612 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 10684..10805 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 10824..10959 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(10997..11013) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 11021..11037 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(11045..11075) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 11090..11111 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(11399..11987) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(12158..13018) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(13019..13123) /gene="bla" /label="AmpR promoter" primer_bind 13597..13613 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgg cggtggactc ggcgatggag ctgttatttt tagatacttt 2341 taaacacccg agcgctgagc aaagttctca tatagatgtg gttcgttttc catgtgtggt 2401 ttatatcaat gaagtccgag tcataccccc aggagtaaga gcccatagca gtctgccaga 2461 caatagagca tatggagaga catctcccca tacatttcaa ttagacttat tcttcaacaa 2521 tgtaagcaaa ccaagtgccc ctgttttcga taggttggga agcctggaat atgatgagaa 2581 tacttccatc atctttagac ctaactcaaa ggtgaatact gatggtctgg tgctaagagg 2641 ctggtataac tgtctgacac tggcaatata tggatcagtg gatagagtga taagtcatga 2701 cagagactct ccaccaccac cacctccacc gccaccacct ccccagccac aaccaagttt 2761 gaaaaggaat ccaaaacatg ctgatgggga gaaagaagat cagtttaatg gaagccctcc 2821 aagaccacag ccaaggggac caagaactcc tccaggaccc cctccacctg atgatgatga 2881 agatgatcct gtgcctctgc cagtgtctgg tgacaaggaa gaggatgctc ctcatagaga 2941 agattacttt gagcccattt ctcctgatcg gaattctgtt ccccaggaag ggcaatattc 3001 tgatgaagga gaagtagaag aggaacaaca agaagaagga gaagaagatg aagatgatgt 3061 ggatgtagag gaagaagagg atgaggatga ggatgatcga cgaacagtag acagtattcc 3121 tgaggaggaa gaggaagatg aagaggaaga aggtgaagag gatgaagaag gtgaagggga 3181 tgatggttat gaacaaattt ccagtgatga agatggaatt gctgacttgg aacgtgaaac 3241 atttaagtat ccaaactttg atgttgaata cactgctgaa gacttagctt cagttcctcc 3301 tatgacatat gatccatatg acagggagct tgtaccactc ttatacttca gttgtccata 3361 caagactact tttgaaattg aaatcagtag aatgaaggat caaggtccag ataaagaaaa 3421 ttcaggggca atcgaagcct cagtgaagtt aacagaactc ttagatttgt atagagaaga 3481 tagaggtgca aaatgggtaa cagctttaga agaaattcca agtttaataa taaaagggtt 3541 aagctatttg caattgaaaa acacaaaaca agactccctt ggccagttgg tagactggac 3601 catgcaagct ttaaatttac aagtagcgct tcgccaacct atcgccttaa atgttcgaca 3661 gctcaaagct gggaccaaat tagtgtcctc actagcagaa tgtggggctc aaggagttac 3721 aggactgcta caagcaggag tgatcagtgg attatttgaa cttctgtttg ctgatcacgt 3781 atcatcttct cttaagttaa atgcttttaa agctttggac agtgtcatta gtatgacaga 3841 aggaatggaa gcttttttaa gaggtaggca gaatgaaaaa agtggttatc aaaagcttct 3901 ggaactcata cttttagatc agactgtgag ggttgttact gctggttcag ctattctcca 3961 aaaatgccat ttctatgaag tcttgtcaga gattaaaaga cttggtgacc atttagcaga 4021 gaagacttca tctcttccta accacagtga acctgatcac gacacagatg ctggacttga 4081 gagaacaaac ccagaatatg aaaatgaggt ggaagcttct atggatatgg atcttttgga 4141 atcctcaaat ataagtgaag gggaaataga aaggcttatt aacctcctag aagaagtttt 4201 tcatttaatg gaaactgccc ctcatacaat gatccaacaa cctgttaagt ctttcccaac 4261 gatggcacga attactggac ctccagagag ggatgatcca taccctgttc tctttagata 4321 tcttcacagt catcacttct tggagttggt taccttgctt ctgtcaattc cagtaacaag 4381 tgctcaccct ggtgtgctgc aagccacaaa agatgttttg aagtttcttg cacagtcaca 4441 gaagggtctt ctttttttta tgtcggaata tgaagcaaca aatttattga tccgagctct 4501 gtgtcacttt tatgatcaag atgaggagga aggtctccaa tctgatggtg ttattgatga 4561 tgcatttgcc ttgtggctac aggactcaac acagacattg caatgtatta cagaactgtt 4621 cagccatttt cagcgttgta cagccagtga agaaacagac cattcagatc tcttgggaac 4681 cctgcacaat ctttatttga ttacttttaa tcctgtggga agatcagctg ttggccatgt 4741 ttttagtctg gagaaaaatc tccaaagtct tattactcta atggagtact attccaaaga 4801 agccttgggt gattccaaat ctaagaagtc agtagcttat aattacgcat gcatacttat 4861 tttggtggtg gttcagtctt ccagtgatgt tcaaatgcta gaacaacatg cagcatctct 4921 cttgaagctt tgtaaagcag atgaaaataa tgctaaattg caagaacttg gcaagtggct 4981 tgaacctctg aaaaacctta gatttgaaat taactgcatc ccaaacttaa ttgagtatgt 5041 taagcagaat atcgataact tgatgacccc agaaggagtt ggccttacca ctgccttacg 5101 tgttctctgt aatgttgcat gcccaccacc tcctgttgaa ggtcaacaga aagatctgaa 5161 atggaatctt gccgttattc agcttttttc tgctgaagga atggacacgt ttattcgagt 5221 tctgcaaaaa ttgaacagta ttctgactca gccttggagg ctccatgtca acatggggac 5281 tacccttcac agagttacta ctatttcaat ggctcgctgc acactcactc ttcttaaaac 5341 tatgttaacg gaactcctga gaggtggatc ctttgagttt aaggacatgc gtgttccttc 5401 agcgcttgtt actttacata tgctcctgtg ctctatcccc ctctcaggtc gtttggatag 5461 tgatgaacag aaaattcaga atgatatcat tgatatttta ctgactttta cacaaggagt 5521 taatgaaaaa ctcacaatct cagaagagac tctggccaat aatacttggt ctttaatgtt 5581 aaaagaagtt ctttcttcaa tcttgaaggt tcctgaagga tttttttctg gactcatact 5641 cctttcagag ctgctgcctc ttccattgcc catgcaaaca actcaggtta ttgagccaca 5701 tgatatatca gtggcactca acacccgaaa attgtggagc atgcaccttc atgttcaagc 5761 aaagttgctc caagaaatag ttcgctcttt ctctggcaca acctgccagc ccattcaaca 5821 tatgttacgg cgtatttgtg ttcaattgtg tgaccttgcc tcaccaactg cacttctgat 5881 tatgagaact gtgttggatt tgattgtaga agacttgcaa agcacttcag aagataaaga 5941 aaaacagtat actagccaaa ccaccaggtt gcttgctctt cttgatgctc tggcttcaca 6001 caaagcttgt aaattagcta ttttgcatct aattaatgga actattaaag gtgatgaaag 6061 atatgcagag atattccagg atcttttagc tttggtgcgg tctcctggag acagtgttat 6121 tcgccaacag tgtgttgaat atgtcacatc cattttgcag tctctctgtg atcaggacat 6181 tgcacttatc ttaccaagct cttctgaagg ttctatttct gaactggagc agctctccaa 6241 ttctctacca aataaagaat tgatgacctc aatctgtgac tgtctgttgg ctacgctagc 6301 taactctgag agcagttaca actgtttact gacatgtgtc agaacaatga tgtttcttgc 6361 agagcatgat tatggattat ttcatttaaa aagttcttta aggaaaaaca gtagtgctct 6421 gcatagttta ctgaaacgag tggtcagcac atttagtaag gacacaggag agcttgcatc 6481 ttcattttta gaatttatga gacaaattct taactctgac acaattggat gctgtggaga 6541 tgataatggt ctcatggaag tagagggagc tcatacatca cggacgatga gtattaatgc 6601 tgcagagtta aaacagcttc tacaaagcaa agaagaaagt ccagaaaatt tgttccttga 6661 actagagaag cttgttttgg aacattcaaa agatgatgac aatctggatt ctttgttgga 6721 cagtgtagtt ggacttaagc agatgctgga gtcatcaggt gaccctttac ctctcagtga 6781 ccaggatgta gaaccagtac tttcagctcc agaatctctt cagaatctgt ttaacaatag 6841 gactgcctat gtgcttgctg atgtcatgga tgatcagttg aaatctatgt ggttcactcc 6901 atttcaggct gaagagatag atacagatct ggatttggta aaggttgact taattgaact 6961 ctctgaaaaa tgctgtagtg actttgattt gcactcagaa ttagagcgct catttttgtc 7021 agaaccatca tctccaggaa gaaccaagac tactaaagga ttcaaacttg ggaagcacaa 7081 gcatgagacc tttataacgt caagtggaaa atctgaatac attgaacctg ccaaaagagc 7141 tcatgttgtg ccaccaccaa gaggaagggg caggggagga tttggacagg gtatacgacc 7201 tcatgatatt tttcgtcaga gaaaacagaa cacaagtaga ccaccatcta tgcatgtgga 7261 tgactttgtt gctgctgaaa gtaaagaagt ggttcctcaa gatggaatac ctccaccaaa 7321 acggccactc aaagtatcac agaagatttc ttcccgtggt gggttttcag gcaatagagg 7381 aggacggggt gctttccaca gtcagaatag gtttttcaca ccacctgctt caaaaggaaa 7441 ctacagtcgt cgggaaggaa caagaggctc cagttggagt gctcagaata ctcctcgagg 7501 aaattacaat gaaagtcgtg gaggccagag caattttaac agaggccctc ttccaccatt 7561 acgacccctt agttctacag gttaccgccc aagtcctcgg gaccgtgctt ctagaggtcg 7621 tgggggactt ggaccttcct gggctagtgc aaatagcggc agtggaggct caagaggaaa 7681 gtttgttagt ggaggcagtg gtagaggtcg tcatgtacgc tcctttacac gataggatcc 7741 gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 7801 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 7861 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 7921 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 7981 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 8041 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 8101 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 8161 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 8221 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 8281 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 8341 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 8401 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 8461 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 8521 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 8581 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 8641 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 8701 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 8761 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 8821 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 8881 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 8941 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 9001 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 9061 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 9121 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 9181 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 9241 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 9301 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 9361 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 9421 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 9481 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 9541 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 9601 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 9661 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 9721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 9781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 9841 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 9901 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 9961 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 10021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 10081 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 10141 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 10201 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc 10261 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga 10321 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 10381 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 10441 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 10501 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 10561 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 10621 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 10681 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 10741 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 10801 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 10861 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 10921 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 10981 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 11041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 11101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 11161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 11221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 11281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 11341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 11401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 11461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 11521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 11581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 11641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 11701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 11761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 11821 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 11881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 11941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 12001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 12061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 12121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 12181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 12241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 12301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 12361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 12421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 12481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 12541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 12601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 12661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 12721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 12781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 12841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 12901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 12961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 13021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 13081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 13141 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 13201 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 13261 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 13321 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 13381 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 13441 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 13501 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 13561 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 13621 tg //