LOCUS       V047482                11113 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047482
VERSION     V047482
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5967
                     /label="VCPIP1(NM_025054)"
                     /note="VCPIP1(NM_025054)"
                     /gene="VCPIP1"
     CDS             5974..6039
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6083..6294
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6307..6575
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6602..7201
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7208..7796
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7870..8103
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8175..8296
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8315..8450
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8488..8504)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8512..8528
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8536..8566)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8581..8602
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8890..9478)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9649..10509)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10510..10614)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11088..11104
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtctcag ccgccgccgc cgccgcctcc gttgccgccg
     2341 ccacctcctc cccctgaggc tccacagact ccgtcgtcct tggcgtcggc ggctgcttcg
     2401 ggggggcttt tgaagcggag agaccggaga atcctttccg ggagctgccc ggatccgaag
     2461 tgtcaggcgc gtctattttt cccggcctcc ggttctgtca gcatcgagtg taccgagtgc
     2521 ggccagcggc acgagcagca acagctgctg ggggttgagg aggtgaccga cccggacgta
     2581 gtgctacaca acctgctgcg gaacgcgctg ctcggggtta cgggggcacc caagaagaac
     2641 acggaactgg taaaggtgat gggcctttcc aactatcact gcaaattgtt gtcgcccata
     2701 ttagctcgct atggaatgga caaacagaca ggccgggcca agcttctccg ggacatgaac
     2761 cagggcgaac tgttcgattg cgccttactg ggtgaccgcg ccttcctcat agaaccagag
     2821 catgttaaca ctgtgggcta tggcaaggac cgctccggaa gcctcctgta tttgcatgac
     2881 actctggagg acattaagcg ggccaataaa agccaggaat gtctcattcc agtgcatgtg
     2941 gacggggatg gacactgctt ggtgcatgct gtgtctcggg ctctagtagg ccgagagctc
     3001 ttctggcatg ccttaagaga gaatcttaaa cagcactttc agcagcacct ggcccgatat
     3061 caagctctgt tccatgactt cattgatgct gctgagtggg aggacattat caatgagtgt
     3121 gaccctctgt ttgtaccacc tgagggtgtt cccttgggcc tgaggaatat ccacatattt
     3181 ggtcttgcca atgtgctaca tcgtcctatt attctgttag attccctcag tggcatgaga
     3241 agctctggtg attattcagc cacctttcta cctgggctca tccctgcaga gaagtgcact
     3301 gggaaagatg gtcatttgaa caaaccaatc tgtattgcat ggagcagctc cggtagaaac
     3361 cattatatcc ccttggtagg cataaaaggg gctgctttgc ccaaactgcc tatgaatttg
     3421 cttcctaaag catggggtgt gcctcaggac cttattaaaa agtacataaa acttgaagag
     3481 gatggtggtt gtgttattgg aggtgacaga agtttgcaag ataaatactt acttaggctt
     3541 gttgctgcta tggaagaagt ctttatggac aaacatggta tccatcctag tttggttgct
     3601 gatgtccatc agtatttcta cagaaggact ggagtgatag gagttcagcc tgaggaagtc
     3661 acagcagctg ctaaaaaagc agtaatggat aatcgccttc acaaatgttt gctctgtggt
     3721 gccctttctg aacttcatgt tcctccagag tggttggctc ctggagggaa attgtataac
     3781 ctggcaaaaa gtactcatgg acagctgagg actgacaaaa attacagctt tcccttgaac
     3841 aatttggttt gctcatatga ttcagtgaaa gatgttctgg taccagacta tggaatgagt
     3901 aacctaacag cttgtaattg gtgccatggc acatctgtgc gaaaggtcag aggagatggg
     3961 tctattgtgt atttggatgg agacagaact aattctaggt ccactggtgg caaatgtggt
     4021 tgtggattca aacacttttg ggatggtaag gagtatgaca atctaccaga agctttccct
     4081 attactttag aatggggtgg aagagtggtc agagaaacag tatattggtt ccagtatgaa
     4141 agtgattcat ctttgaatag taatgtttac gatgttgcaa tgaaacttgt taccaagcac
     4201 tttccaggtg aatttgggag tgaaatccta gttcagaaag ttgtccacac tatattgcat
     4261 cagactgcca aaaagaatcc cgatgattat actcctgtaa atatagatgg tgctcacgcc
     4321 caaagagttg gagatgttca aggacaagaa tcagagtctc agctcccaac taaaattatt
     4381 cttactggac agaaaacaaa aactttgcac aaggaggagt taaacatgag taaaactgaa
     4441 agaactattc aacagaatat tacggaacag gcttctgtaa tgcagaaacg gaaaacagag
     4501 aagttaaaac aagaacaaaa agggcaaccc aggactgttt ctcccagtac cattcgtgat
     4561 ggtccatcct ctgcacctgc tacacctacc aaggctccct attcaccgac aacttctaag
     4621 gagaagaaga tccgaatcac aactaatgat ggacgacagt ccatggttac ccttaagtct
     4681 tcaacaacct tttttgaact tcaggaaagt atagccagag aattcaacat tcctccatat
     4741 ttacagtgta ttcgatacgg gtttcctcct aaagagttaa tgccaccaca ggcaggaatg
     4801 gaaaaggaac cagttccttt acagcatggc gacagaatta caatagaaat tctaaaaagt
     4861 aaagctgaag gtggtcagtc tgctgcagca cactcagccc acactgtgaa acaagaagat
     4921 attgctgtta ctggtaaact gtcatctaag gaacttcagg agcaagctga aaaagaaatg
     4981 tactccttgt gtcttttagc aacattaatg ggagaagatg tatggtctta tgcaaaggga
     5041 cttcctcaca tgtttcagca gggtggtgta ttctacagta ttatgaagaa aaccatgggt
     5101 atggctgatg gcaagcattg tacttttcca catctgcctg gcaaaacctt tgtctataat
     5161 gcttctgaag atagactgga attgtgtgtg gatgctgcag gacatttccc cattggtcct
     5221 gatgttgaag atttagttaa agaggctgta agtcaggttc gagcagaggc tactacaaga
     5281 agtagggaat caagtccctc acatgggcta ttaaaactag gtagtggtgg agtagtgaaa
     5341 aagaaatctg agcaacttca taacgtaact gcctttcagg gaaaagggca ttctttagga
     5401 actgcatctg gtaacccaca ccttgatcca agagctaggg aaacttcagt tgtaagaaag
     5461 cataatacag ggacagactt tagtaatagt tccactaaaa cagagccttc tgtattcaca
     5521 gcttcttcta gtaatagtga gcttattcga atagctcctg gagtagtaac aatgagagac
     5581 ggcaggcagc ttgatcctga tttggttgag gcccagcgaa aaaaattgca ggaaatggtt
     5641 tcttctattc aggcttcaat ggacaggcac cttcgggatc aaagtacaga gcagtcacca
     5701 tctgatcttc ctcaaaggaa aacagaagtt gtgagttctt ctgcaaagtc tgggagtctt
     5761 cagactggtt tgcctgaatc ttttccttta actggtggta ctgaaaattt gaatacagaa
     5821 acaactgatg gctgtgtagc agatgcactg ggagcagcct ttgccacaag gtcaaaagca
     5881 caaaggggaa attccgtgga ggagcttgaa gagatggata gtcaagatgc tgagatgact
     5941 aacacaactg agccaatgga tcactctgga tccgactaca aagaccatga cggtgattat
     6001 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     6061 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     6121 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     6181 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6241 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     6301 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     6361 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     6421 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     6481 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     6541 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     6601 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     6661 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     6721 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6781 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6841 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6901 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6961 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7021 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7081 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7141 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7201 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7261 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7321 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7381 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7441 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7501 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7561 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7621 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7681 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7741 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7801 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7861 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     7921 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7981 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8041 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8101 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8161 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8221 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8281 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     8341 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8401 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     8461 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8521 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8581 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8641 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8701 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8761 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8821 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8881 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8941 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9001 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9061 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9121 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9181 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9241 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9301 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9361 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9421 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9481 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9541 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9601 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9661 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9721 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9781 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9841 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9901 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9961 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10021 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10081 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10141 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10201 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10261 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10321 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10381 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10441 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10501 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10561 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10621 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10681 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10741 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10801 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10861 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10921 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10981 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    11041 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11101 cagtgccaag ctg
//
