LOCUS       V043313                11233 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043313
VERSION     V043313
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5259
                     /label="USP15(NM_001252078)"
                     /note="USP15(NM_001252078)"
                     /gene="USP15"
     CDS             5266..5331
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5375..5586
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5599..5867
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5896..5905
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5926..6588
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6658..6711
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6712..7311
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7312..7900
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7974..8207
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8279..8400
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8440..8575
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8608..8624)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8632..8648
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8656..8686)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8701..8722
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9010..9598)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9769..10629)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10630..10734)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11208..11224
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggaaggcgg agcggcggat
     2341 ctggacaccc agcggtctga catcgcgacg ctgctcaaaa cctcgctccg gaaaggggac
     2401 acctggtacc tagtcgatag tcgctggttc aaacagtgga aaaaatatgt tggctttgac
     2461 agttgggaca aataccagat gggagatcaa aatgtgtatc ctggacccat tgataactct
     2521 ggacttctca aagatggtga tgcccagtca cttaaggaac accttattga tgaattggat
     2581 tacatactgt tgccaactga aggttggaat aaacttgtca gctggtacac attgatggaa
     2641 ggtcaagagc caatagcacg aaaggtggtt gaacagggta tgtttgtaaa gcactgcaaa
     2701 gtagaagtat atctcacaga attgaagcta tgtgaaaatg gaaacatgaa taatgttgta
     2761 actcgaagat ttagcaaagc tgacacaata gatacaattg aaaaggaaat aagaaaaatc
     2821 ttcagtattc cagatgaaaa ggagaccaga ttgtggaaca aatacatgag taacacattt
     2881 gaaccactga ataaaccaga cagcaccatt caggatgctg gtttatacca aggacaggta
     2941 ttagtgatag aacagaaaaa tgaagatgga acatggccaa ggggtccttc tactcctaag
     3001 tccccaggtg catccaattt ttcaacttta ccaaagatct ctccttcatc tctatcaaat
     3061 aattataaca acatgaacaa cagaaatgtg aaaaactcaa attactgtct tccatcatat
     3121 accgcttata agaactatga ttattcggaa cctggaagaa acaatgaaca gccaggcctc
     3181 tgtggcctaa gtaacttggg aaatacgtgt ttcatgaact cagctattca gtgtttgagc
     3241 aacacacctc cacttactga gtatttcctc aatgataagt atcaagaaga actgaatttt
     3301 gacaatccct taggaatgag aggtgaaata gctaaatctt atgccgaact gatcaagcaa
     3361 atgtggtctg gaaagtttag ctacgtcacc ccaagagcct ttaagacaca ggtaggacgt
     3421 tttgcacctc agttctctgg atatcagcag caagactgtc aagaactgtt agctttccta
     3481 ttagatggat tacatgagga tttgaataga attaggaaaa aaccatatat acaattaaaa
     3541 gatgcagatg gaaggccaga taaggtggtt gccgaagaag cctgggaaaa ccatttaaaa
     3601 cgaaatgatt ctatcatagt agatatattt catggccttt tcaaatcaac tttagtttgt
     3661 cctgagtgtg ctaagatttc agtaacattt gatccttttt gttacttgac acttccattg
     3721 cccatgaaaa aagaacgcac cttggaagtt tacttagtta gaatggatcc acttaccaaa
     3781 cctatgcagt acaaagtggt tgtccccaaa attggaaaca tattagatct ttgtacagca
     3841 ttgtctgctt tgtcaggaat acctgcagat aagatgatag ttactgatat atacaatcat
     3901 agatttcaca gaatattcgc tatggatgaa aaccttagta gtattatgga acgggatgat
     3961 atttatgtgt ttgaaattaa catcaatagg acagaagata cagagcacgt gattattcct
     4021 gtttgcctaa gagaaaaatt cagacactcg agttataccc accatactgg ttcttcactt
     4081 tttggtcagc cctttcttat ggctgtacca cgaaacaata ctgaagacaa actttataat
     4141 ctcctgctct tgagaatgtg ccgatatgtc aaaatatcta ctgaaactga agaaactgaa
     4201 ggatccctac actgctgtaa ggaccaaaat attaatggga atggcccaaa tggcatacat
     4261 gaagaaggct caccaagtga aatggaaaca gatgagccag atgatgaatc cagccaggat
     4321 caagaacttc cctcagagaa tgaaaacagt cagtctgaag attcagttgg aggagataat
     4381 gattctgaaa atggattatg tactgaggat acttgcaaag gtcaactcac gggacacaaa
     4441 aaacgattgt ttacattcca gttcaacaac ttaggcaata ctgatatcaa ctacatcaaa
     4501 gatgatacca ggcatataag atttgatgat aggcagctta ggctagatga aagatctttt
     4561 cttgctttgg attgggatcc tgatttgaaa aaaagatatt ttgatgaaaa tgctgctgag
     4621 gactttgaaa aacatgaaag tgtggagtat aaacctccta aaaaaccctt tgtgaaatta
     4681 aaagattgca ttgaactttt tacaacaaaa gaaaagctag gtgctgaaga tccctggtat
     4741 tgtccgaatt gtaaagaaca tcagcaagcc acaaagaaat tggatttatg gtccctgcct
     4801 ccagtacttg tagtacatct caagcgattt tcttacagtc gatacatgag agacaagttg
     4861 gataccttag ttgattttcc tatcaatgac ttggatatgt cggaattctt aattaatcca
     4921 aatgcaggtc cttgccgcta taatctgatt gctgtttcca accactatgg agggatggga
     4981 ggaggacact atactgcttt tgcaaaaaat aaagatgatg gaaaatggta ctattttgat
     5041 gacagtagtg tctccactgc atctgaagac caaattgtgt ccaaagcagc atatgtactc
     5101 ttctaccaga gacaagacac tttcagtgga actggctttt ttcctcttga ccgagaaact
     5161 aaaggtgctt cagctgccac tggcatccca ttagaaagtg atgaagatag caatgataat
     5221 gacaatgata tagaaaatga aaactgtatg cacactaacg gatccgacta caaagaccat
     5281 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     5341 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5401 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5461 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5521 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5581 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     5641 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5701 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5761 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5821 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5881 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     5941 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     6001 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     6061 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     6121 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     6181 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     6241 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     6301 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     6361 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     6421 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     6481 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     6541 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     6601 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     6661 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     6721 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6781 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6841 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6901 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6961 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7021 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7081 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7141 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7201 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7261 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     7321 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7381 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7441 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7501 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7561 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7621 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7681 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7741 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7801 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7861 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     7921 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7981 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     8041 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8101 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8161 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8221 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8281 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8341 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8401 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     8461 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8521 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     8581 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     8641 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8701 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8761 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8821 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8881 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8941 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9001 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9061 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9121 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9181 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9241 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9301 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9361 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9421 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9481 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9541 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9601 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9661 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9721 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9781 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9841 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9901 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9961 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10021 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10081 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10141 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10201 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10261 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10321 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10381 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10441 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10501 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10561 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10621 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10681 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10741 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10801 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10861 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10921 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10981 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    11041 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    11101 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    11161 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11221 cagtgccaag ctg
//
