LOCUS       V030843                11107 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030843
VERSION     V030843
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5133
                     /label="Trak1(NM_001134565)"
                     /note="Trak1(NM_001134565)"
                     /gene="Trak1"
     CDS             5140..5205
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5249..5460
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5473..5741
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5770..5779
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5800..6462
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6532..6585
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6586..7185
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7186..7774
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7848..8081
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8153..8274
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8314..8449
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8482..8498)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8506..8522
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8530..8560)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8575..8596
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8884..9472)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9643..10503)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10504..10608)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11082..11098
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cattggcgat tcagctcagg
     2341 cagccctcca gggcccaacc tctgccggga ctcgaccaca cactggctgg gacaaactcc
     2401 tgtgacgtgt gcaacagcac caaccttcca gaagtggaga tcattagcct gctggaggag
     2461 cagctgcccc actacaagct aagagcggac accatctatg gttatgacca cgatgactgg
     2521 ctgcacacgc ccctcatctc cccagatgcc aacatcgacc tcacaaccga gcagatcgaa
     2581 gagacgctga agtacttcct tctatgtgcc gaaagagttg gccagatgac taagacatac
     2641 aatgacattg atgctgtcac aaggcttctt gaggagaaag agcgggattt ggagctggct
     2701 gcgaggatcg gccagtcatt gttgaagaag aacaagaccc taactgagag gaacgaactg
     2761 ctggaagagc aggtggagca catccgggaa gaggtgtctc agctccgcca tgagctgtcc
     2821 atgaaggatg agctgcttca gttctacacc agtgccgcgg aggagagtga gcctgagtct
     2881 gtctgctcaa ccccgctgaa gaggaacgag tcatcctcct ccgtccagaa ttacttccac
     2941 ctggactctc ttcagaagaa gctgaaggac ctcgaagagg agaacgtcgt acttcgatct
     3001 gaggcttgtc agctgaagac ggagaccatc acctatgagg agaaggagca gcagctggtg
     3061 aacgactgtg tgaaggagct gagggatgct aacgtccaga ttgcgagcat ctctgaggag
     3121 ctggccaaga agacggaaga tgccgcccgc cagcaggagg agatcacgca cctgctgtcg
     3181 caaatcgtgg acttgcagaa gaaggcgaaa tcctgtgcgg tggaaaatga agaacttgtc
     3241 cagcacctgg gcgctgccaa ggatgcgcag cgccaactca cagccgagct tcgagagctg
     3301 gaggataagt acgccgaatg catggagatg cttcatgagg ctcaggagga actgaagaac
     3361 ctgcggaaca agacgatgcc cacatcccgg cgctaccact ccctgggcct gttccccatg
     3421 gactccttgg ctgccgaaat tgagggaacc atgcgcaagg agctgcagct ggaagagccg
     3481 gagtccccag acatcaccca ccagaagcgt gtctttgaga cggtgagaaa tgtcaatcaa
     3541 gtggtcaagc agagatcgct aaccccttcc cccatgaaca tcccgggctc caaccagtct
     3601 tcagccatga actccctcct gtccagctgc gtcagcaccc cccgttccag cttctacggc
     3661 agcgatgtca gccacgtggt cctcgataac aaaaccaaca gcatcctcct ggaaactgag
     3721 gcagccgacc tggggaatga ggagcacaat aagaagccgg gcacaccagg cacaccaggc
     3781 tcccatgacc tggagacggc acttaggcgg ctatccctgc gccgagagaa ctacctatct
     3841 gagcggaggt tcttcgagga ggagcaggag aggaagctgc gagagctggc tgagaaggga
     3901 gagctgcaca gtggctcgct cacgcccacc gagagcatca tgtccctggg cacacactca
     3961 cgcttctccg agttcacggg cttctccggc atgtccttca gcagccgctc ctacctgccc
     4021 gagaagctgc agatcgtgaa gccactcgaa ggttcagcca cacttcacca ttggcagcaa
     4081 ttggcccagc ctcacctcgg gggcatcctg gacccccgcc ctggtgtggt caccaagggc
     4141 ttccggactt tggacgttga cctggatgaa gtgtactgcc ttaacgactt tgaggaagat
     4201 gacacaggtg accacatttc tctggcgggc ctagctacct ccacaacaat tcagcacatg
     4261 gagacctcag cgcaccaccc tgggaagtgc atgtcacaga ccaactcgac tttcaccttt
     4321 accacctgcc gcatcctgca cccttcagac gagctcacgc gggtcacgcc aagccttaac
     4381 tcagccccag ctccatcttg tagcagcacc agccacttga aatccacgcc ggtggccacg
     4441 ccatgcaccc cccggagact gagcctggcc gagtccttca caaacgtccg cgagtccacg
     4501 acaaccatga gcacgtccct ggggctggtg tggttgctga aggaacgagg catctcagcg
     4561 gctgtgtacg acccccagag ttgggacaga gccggcaggg gctccctcct ccactcttat
     4621 acccctagga tggctgtgat cccgtccacc ccaccaaatt cccctatgca gacgccctcg
     4681 gcctccccgc cctccttcga gttcaagtgc acgagccctc cctacaacaa cttcctggct
     4741 tccaagcctg ccagctccat cctgagggag gtgagggaga agaagcacgt gaggagcagt
     4801 gagagccaga cggacgtgtc tgtctccaac cttaaccttg tggacaaagt caggaggttt
     4861 ggggtggcca gagtggtgaa ctcaggacga gcccacatcc ccaccttgac tgaggatcag
     4921 ggacctctcc tctgtggacc gaccgggcca gcacacgctc ttgtccctgg gggcctggtc
     4981 cccgagggac tgcctcttgg gtgccccagt ggcatccgac ggaatcgcag cttccccacc
     5041 atggtcgggt ccagcgtgca gatgagggct cctgtaatcc tcacctctgg catcttaatg
     5101 ggtgctaagc tccccaaaca gactagcttg cggggatccg actacaaaga ccatgacggt
     5161 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5221 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5281 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5341 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5401 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5461 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5521 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5581 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5641 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5701 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5761 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5821 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5881 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5941 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     6001 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6061 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6121 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6181 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6241 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6301 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6361 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6421 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6481 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6541 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6601 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6661 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6721 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6781 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6841 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6901 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6961 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7021 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7081 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7141 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7201 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7261 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7321 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7381 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7441 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7501 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7561 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7621 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7681 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7741 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7801 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7861 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7921 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7981 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8041 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8101 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8281 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8341 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8401 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     8461 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9301 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10621 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10681 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10741 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10801 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10861 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10921 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10981 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11041 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11101 caagctg
//
