LOCUS       V046356                10865 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046356
VERSION     V046356
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2311..2320
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2320..2391
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2398..4914
                     /label="TRPV1(NM_018727)"
                     /note="TRPV1(NM_018727)"
                     /gene="TRPV1"
     CDS             4930..4950
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /transl_table=1
                     /translation="ENLYFQG"
                     /label="TEV site"
     CDS             4957..4980
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     promoter        5024..5235
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5248..5516
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5545..5554
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5575..6237
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6307..6360
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6361..6960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6961..7548
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7622..7855
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7927..8048
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8067..8202
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8240..8256)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8264..8280
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8288..8318)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8333..8354
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8642..9230)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9401..10261)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10262..10366)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10840..10856
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg actacaagga tgacgatgac
     2341 aaggattaca aagacgacga tgataaggac tataaggatg atgacgacaa agaattcatg
     2401 aagaaatgga gcagcacaga cttgggggca gctgcggacc cactccaaaa ggacacctgc
     2461 ccagaccccc tggatggaga ccctaactcc aggccacctc cagccaagcc ccagctctcc
     2521 acggccaaga gccgcacccg gctctttggg aagggtgact cggaggaggc tttcccggtg
     2581 gattgccctc acgaggaagg tgagctggac tcctgcccga ccatcacagt cagccctgtt
     2641 atcaccatcc agaggccagg agacggcccc accggtgcca ggctgctgtc ccaggactct
     2701 gtcgccgcca gcaccgagaa gaccctcagg ctctatgatc gcaggagtat ctttgaagcc
     2761 gttgctcaga ataactgcca ggatctggag agcctgctgc tcttcctgca gaagagcaag
     2821 aagcacctca cagacaacga gttcaaagac cctgagacag ggaagacctg tctgctgaaa
     2881 gccatgctca acctgcacga cggacagaac accaccatcc ccctgctcct ggagatcgcg
     2941 cggcaaacgg acagcctgaa ggagcttgtc aacgccagct acacggacag ctactacaag
     3001 ggccagacag cactgcacat cgccatcgag agacgcaaca tggccctggt gaccctcctg
     3061 gtggagaacg gagcagacgt ccaggctgcg gcccatgggg acttctttaa gaaaaccaaa
     3121 gggcggcctg gattctactt cggtgaactg cccctgtccc tggccgcgtg caccaaccag
     3181 ctgggcatcg tgaagttcct gctgcagaac tcctggcaga cggccgacat cagcgccagg
     3241 gactcggtgg gcaacacggt gctgcacgcc ctggtggagg tggccgacaa cacggccgac
     3301 aacacgaagt ttgtgacgag catgtacaat gagattctga tgctgggggc caaactgcac
     3361 ccgacgctga agctggagga gctcaccaac aagaagggaa tgacgccgct ggctctggca
     3421 gctgggaccg ggaagatcgg ggtcttggcc tatattctcc agcgggagat ccaggagccc
     3481 gagtgcaggc acctgtccag gaagttcacc gagtgggcct acgggcccgt gcactcctcg
     3541 ctgtacgacc tgtcctgcat cgacacctgc gagaagaact cggtgctgga ggtgatcgcc
     3601 tacagcagca gcgagacccc taatcgccac gacatgctct tggtggagcc gctgaaccga
     3661 ctcctgcagg acaagtggga cagattcgtc aagcgcatct tctacttcaa cttcctggtc
     3721 tactgcctgt acatgatcat cttcaccatg gctgcctact acaggcccgt ggatggcttg
     3781 cctcccttta agatggaaaa aactggagac tatttccgag ttactggaga gatcctgtct
     3841 gtgttaggag gagtctactt ctttttccga gggattcagt atttcctgca gaggcggccg
     3901 tcgatgaaga ccctgtttgt ggacagctac agtgagatgc ttttctttct gcagtcactg
     3961 ttcatgctgg ccaccgtggt gctgtacttc agccacctca aggagtatgt ggcttccatg
     4021 gtattctccc tggccttggg ctggaccaac atgctctact acacccgcgg tttccagcag
     4081 atgggcatct atgccgtcat gatagagaag atgatcctga gagacctgtg ccgtttcatg
     4141 tttgtctaca tcgtcttctt gttcgggttt tccacagcgg tggtgacgct gattgaagac
     4201 gggaagaatg actccctgcc gtctgagtcc acgtcgcaca ggtggcgggg gcctgcctgc
     4261 aggccccccg atagctccta caacagcctg tactccacct gcctggagct gttcaagttc
     4321 accatcggca tgggcgacct ggagttcact gagaactatg acttcaaggc tgtcttcatc
     4381 atcctgctgc tggcctatgt aattctcacc tacatcctcc tgctcaacat gctcatcgcc
     4441 ctcatgggtg agactgtcaa caagatcgca caggagagca agaacatctg gaagctgcag
     4501 agagccatca ccatcctgga cacggagaag agcttcctta agtgcatgag gaaggccttc
     4561 cgctcaggca agctgctgca ggtggggtac acacctgatg gcaaggacga ctaccggtgg
     4621 tgcttcaggg tggacgaggt gaactggacc acctggaaca ccaacgtggg catcatcaac
     4681 gaagacccgg gcaactgtga gggcgtcaag cgcaccctga gcttctccct gcggtcaagc
     4741 agagtttcag gcagacactg gaagaacttt gccctggtcc cccttttaag agaggcaagt
     4801 gctcgagata ggcagtctgc tcagcccgag gaagtttatc tgcgacagtt ttcagggtct
     4861 ctgaagccag aggacgctga ggtcttcaag agtcctgccg cttccgggga gaagggtgga
     4921 ggcggatcag agaatctcta cttccaggga ggatcccacc atcaccatca ccatcaccat
     4981 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5041 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     5101 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5161 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5221 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5281 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5341 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5401 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5461 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5521 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5581 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5641 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5701 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5761 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5821 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5881 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     5941 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     6001 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     6061 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     6121 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     6181 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     6241 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     6301 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6361 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6421 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6481 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6541 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6601 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6661 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6721 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6781 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6841 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6901 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6961 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7021 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7081 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7141 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7201 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7261 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7321 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7381 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7441 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc
     7501 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta
     7561 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7621 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     7681 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7741 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7801 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7861 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7921 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7981 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8041 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     8101 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8161 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     8221 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8281 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8341 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8401 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9061 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10381 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10441 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10501 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10561 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    10621 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10681 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    10741 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    10801 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    10861 agctg
//
