LOCUS V046356 10865 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046356 VERSION V046356 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2311..2320 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2320..2391 /codon_start=1 /product="three tandem FLAG® epitope tags" /transl_table=1 /translation="DYKDDDDKDYKDDDDKDYKDDDDK" /label="3xFLAG" CDS 2398..4914 /label="TRPV1(NM_018727)" /note="TRPV1(NM_018727)" /gene="TRPV1" CDS 4930..4950 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /transl_table=1 /translation="ENLYFQG" /label="TEV site" CDS 4957..4980 /codon_start=1 /product="8xHis affinity tag" /transl_table=1 /translation="HHHHHHHH" /label="8xHis" promoter 5024..5235 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5248..5516 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5545..5554 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5575..6237 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6307..6360 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6361..6960 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6961..7548 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7622..7855 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7927..8048 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8067..8202 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8240..8256) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8264..8280 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8288..8318) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8333..8354 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8642..9230) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9401..10261) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10262..10366) /gene="bla" /label="AmpR promoter" primer_bind 10840..10856 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg actacaagga tgacgatgac 2341 aaggattaca aagacgacga tgataaggac tataaggatg atgacgacaa agaattcatg 2401 aagaaatgga gcagcacaga cttgggggca gctgcggacc cactccaaaa ggacacctgc 2461 ccagaccccc tggatggaga ccctaactcc aggccacctc cagccaagcc ccagctctcc 2521 acggccaaga gccgcacccg gctctttggg aagggtgact cggaggaggc tttcccggtg 2581 gattgccctc acgaggaagg tgagctggac tcctgcccga ccatcacagt cagccctgtt 2641 atcaccatcc agaggccagg agacggcccc accggtgcca ggctgctgtc ccaggactct 2701 gtcgccgcca gcaccgagaa gaccctcagg ctctatgatc gcaggagtat ctttgaagcc 2761 gttgctcaga ataactgcca ggatctggag agcctgctgc tcttcctgca gaagagcaag 2821 aagcacctca cagacaacga gttcaaagac cctgagacag ggaagacctg tctgctgaaa 2881 gccatgctca acctgcacga cggacagaac accaccatcc ccctgctcct ggagatcgcg 2941 cggcaaacgg acagcctgaa ggagcttgtc aacgccagct acacggacag ctactacaag 3001 ggccagacag cactgcacat cgccatcgag agacgcaaca tggccctggt gaccctcctg 3061 gtggagaacg gagcagacgt ccaggctgcg gcccatgggg acttctttaa gaaaaccaaa 3121 gggcggcctg gattctactt cggtgaactg cccctgtccc tggccgcgtg caccaaccag 3181 ctgggcatcg tgaagttcct gctgcagaac tcctggcaga cggccgacat cagcgccagg 3241 gactcggtgg gcaacacggt gctgcacgcc ctggtggagg tggccgacaa cacggccgac 3301 aacacgaagt ttgtgacgag catgtacaat gagattctga tgctgggggc caaactgcac 3361 ccgacgctga agctggagga gctcaccaac aagaagggaa tgacgccgct ggctctggca 3421 gctgggaccg ggaagatcgg ggtcttggcc tatattctcc agcgggagat ccaggagccc 3481 gagtgcaggc acctgtccag gaagttcacc gagtgggcct acgggcccgt gcactcctcg 3541 ctgtacgacc tgtcctgcat cgacacctgc gagaagaact cggtgctgga ggtgatcgcc 3601 tacagcagca gcgagacccc taatcgccac gacatgctct tggtggagcc gctgaaccga 3661 ctcctgcagg acaagtggga cagattcgtc aagcgcatct tctacttcaa cttcctggtc 3721 tactgcctgt acatgatcat cttcaccatg gctgcctact acaggcccgt ggatggcttg 3781 cctcccttta agatggaaaa aactggagac tatttccgag ttactggaga gatcctgtct 3841 gtgttaggag gagtctactt ctttttccga gggattcagt atttcctgca gaggcggccg 3901 tcgatgaaga ccctgtttgt ggacagctac agtgagatgc ttttctttct gcagtcactg 3961 ttcatgctgg ccaccgtggt gctgtacttc agccacctca aggagtatgt ggcttccatg 4021 gtattctccc tggccttggg ctggaccaac atgctctact acacccgcgg tttccagcag 4081 atgggcatct atgccgtcat gatagagaag atgatcctga gagacctgtg ccgtttcatg 4141 tttgtctaca tcgtcttctt gttcgggttt tccacagcgg tggtgacgct gattgaagac 4201 gggaagaatg actccctgcc gtctgagtcc acgtcgcaca ggtggcgggg gcctgcctgc 4261 aggccccccg atagctccta caacagcctg tactccacct gcctggagct gttcaagttc 4321 accatcggca tgggcgacct ggagttcact gagaactatg acttcaaggc tgtcttcatc 4381 atcctgctgc tggcctatgt aattctcacc tacatcctcc tgctcaacat gctcatcgcc 4441 ctcatgggtg agactgtcaa caagatcgca caggagagca agaacatctg gaagctgcag 4501 agagccatca ccatcctgga cacggagaag agcttcctta agtgcatgag gaaggccttc 4561 cgctcaggca agctgctgca ggtggggtac acacctgatg gcaaggacga ctaccggtgg 4621 tgcttcaggg tggacgaggt gaactggacc acctggaaca ccaacgtggg catcatcaac 4681 gaagacccgg gcaactgtga gggcgtcaag cgcaccctga gcttctccct gcggtcaagc 4741 agagtttcag gcagacactg gaagaacttt gccctggtcc cccttttaag agaggcaagt 4801 gctcgagata ggcagtctgc tcagcccgag gaagtttatc tgcgacagtt ttcagggtct 4861 ctgaagccag aggacgctga ggtcttcaag agtcctgccg cttccgggga gaagggtgga 4921 ggcggatcag agaatctcta cttccaggga ggatcccacc atcaccatca ccatcaccat 4981 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 5041 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 5101 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 5161 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 5221 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 5281 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 5341 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 5401 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 5461 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 5521 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 5581 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 5641 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 5701 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 5761 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 5821 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 5881 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 5941 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 6001 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 6061 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 6121 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 6181 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 6241 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 6301 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 6361 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6421 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6481 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6541 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6601 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6661 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6721 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6781 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6841 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6901 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6961 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 7021 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 7081 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 7141 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 7201 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 7261 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 7321 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 7381 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 7441 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 7501 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 7561 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 7621 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 7681 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 7741 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7801 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 7861 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 7921 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7981 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 8041 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 8101 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 8161 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 8221 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 8281 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 8341 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 8401 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 8461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 8521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 8581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 8641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 8701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 8761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 8821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 8881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 8941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 9001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 9061 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 9121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 9181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 9241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 9301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 9361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 9421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 9481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 9541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 9601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 9661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 9721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 9781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 9841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 9901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 9961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 10021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 10081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 10141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 10201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 10261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 10321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 10381 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 10441 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 10501 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 10561 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 10621 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 10681 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 10741 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 10801 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 10861 agctg //