LOCUS       V018654                11124 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018654
VERSION     V018654
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5215
                     /label="TRIM37(NM_001353084)"
                     /note="TRIM37(NM_001353084)"
                     /gene="TRIM37"
     CDS             5216..5233
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     promoter        5283..5494
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5507..5775
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5804..5813
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5834..6496
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6566..6619
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6620..7219
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7220..7807
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7881..8114
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8186..8307
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8326..8461
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8499..8515)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8523..8539
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8547..8577)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8592..8613
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8901..9489)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9660..10520)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10521..10625)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11099..11115
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg atgaacagag cgtggagagc attgctgagg ttttccgatg
     2341 tttcatttgt atggagaaat tgcgggatgc acgcctgtgt cctcattgct ccaaactgtg
     2401 ttgtttcagc tgtattaggc gctggctgac agagcagaga gctcaatgtc ctcattgccg
     2461 tgctccactc cagctacgag aactagtaaa ttgtcgttgg gcagaagaag taacacaaca
     2521 gcttgatact cttcaactct gcagtctcac caaacatgaa gaaaatgaaa aggacaaatg
     2581 tgaaaatcac catgaaaaac ttagtgtatt ttgctggact tgtaagaagt gtatctgcca
     2641 tcagtgtgca ctttggggag gaatgcatgg cggacatacc tttaaacctt tggcagaaat
     2701 ttatgagcaa cacgtcacta aagtgaatga agaggtagcc aaacttcgtc ggcgtctcat
     2761 ggaactgatc agcttagttc aagaagtgga aaggaatgta gaagctgtaa gaaatgcaaa
     2821 agatgagcgt gttcgggaaa ttaggaatgc agtggagatg atgattgcac ggttagacac
     2881 acagctgaag aataagctta taacactgat gggtcagaag acatctctaa cccaagaaac
     2941 agagcttttg gaatccttac ttcaggaggt ggagcaccag ttgcggtctt gtagtaagag
     3001 tgagttgata tctaagagct cagagatcct tatgatgttt cagcaagttc atcggaagcc
     3061 catggcatct tttgttacca ctcctgttcc accagacttt accagtgaat tagtgccatc
     3121 ttacgattca gctacttttg ttttagagaa tttcagcact ttgcgtcaga gagcagatcc
     3181 tgtttacagt ccacctcttc aagtttcagg actttgctgg aggttaaaag tttacccaga
     3241 tggaaatgga gttgtgcgag gttactactt atctgtgttt ctggagctct cagctggctt
     3301 gcctgaaact tctaaatatg aatatcgtgt agagatggtt caccagtcct gtaatgatcc
     3361 tacaaaaaat atcattcgag aatttgcatc tgactttgaa gttggagaat gctggggcta
     3421 taatagattt ttccgtttgg acttactcgc aaatgaagga tacttgaatc cacaaaatga
     3481 tacagtgatt ttaaggtttc aggtacgttc accaactttc tttcaaaaat cccgggacca
     3541 gcattggtac attactcagt tggaagctgc acagactagt tatatccaac aaataaacaa
     3601 ccttaaagag agacttacta ttgagctgtc tcgaactcag aagtcaagag atttgtcacc
     3661 accagataac catcttagcc cccaaaatga tgatgctctg gagacacgag ctaagaagtc
     3721 tgcatgctct gacatgcttc tcgaaggtgg tcctactaca gcttctgtaa gagaggccaa
     3781 agaggatgaa gaagatgagg agaagattca gaatgaagat tatcatcacg agctttcaga
     3841 tggagatctg gatctggatc ttgtttatga ggatgaagta aatcagctcg atggcagcag
     3901 ttcctctgct agttccacag caacaagtaa tacagaagaa aatgatattg atgaagaaac
     3961 tatgtctgga gaaaatgatg tggaatataa caacatggaa ttagaagagg gagaactcat
     4021 ggaagatgca gctgctgcag gacccgcagg tagtagccat ggttatgtgg gttccagtag
     4081 tagaatatca agaagaacac atttatgctc cgctgctacc agtagtttac tagacattga
     4141 tccattaatt ttaatacatt tgttggacct taaggaccgg agcagtatag aaaatttgtg
     4201 gggcttacag cctcgcccac ctgcttcact tctgcagccc acagcatcat attctcgaaa
     4261 agataaagac caaaggaagc aacaggcaat gtggcgagtg ccctctgatt taaagatgct
     4321 aaaaagactc aaaactcaaa tggccgaagt tcgatgtatg aaaactgatg taaagaatac
     4381 actttcagaa ataaaaagca gcagtgctgc ttctggagac atgcagacaa gccttttttc
     4441 tgctgaccag gcagctctgg ctgcatgtgg aactgaaaac tctggcagat tgcaggattt
     4501 gggaatggaa ctcctggcaa agtcatcagt tgccaattgt tacatacgaa actccacaaa
     4561 taagaagagt aattcgccca agccagctcg atccagtgta gcaggtagtc tatcacttcg
     4621 aagagcagtg gaccctggag aaaatagtcg ttcaaaggga gactgtcaga ctctgtctga
     4681 aggctcccca ggaagctctc agtctgggag caggcacagt tctccccgag ccttgataca
     4741 tggcagtatc ggtgatattc tgccaaaaac tgaagaccgg cagtgtaaag ctttggattc
     4801 agatgctgtt gtggttgcag ttttcagtgg cttgcctgcg gttgagaaaa ggaggaaaat
     4861 ggtcaccttg ggggctaatg ctaaaggagg tcatctggaa ggactgcaga tgactgattt
     4921 ggaaaataat tctgaaactg gagagttaca gcctgtacta cctgaaggag cttcagctgc
     4981 ccctgaagaa gaaacatgtt ctccttcctt cctgccagga atgagtagcg acagtgacat
     5041 tgaatgtgac actgagaatg aggagcagga agagcatacc agtgtgggcg ggtttcacga
     5101 ctccttcatg gtcatgacac agcccccgga tgaagataca cattccagtt ttcctgatgg
     5161 tgaacaaata ggccctgaag atctcagctt caatacagat gaaaatagtg gaaggcatca
     5221 ccatcatcac cattgaggat ccgcggccgc aaggatctgc gatcgctccg gtgcccgtca
     5281 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     5341 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     5401 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     5461 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     5521 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     5581 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     5641 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     5701 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     5761 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga
     5821 cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt
     5881 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa
     5941 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg
     6001 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc
     6061 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct
     6121 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt
     6181 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc
     6241 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac
     6301 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa
     6361 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt
     6421 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac
     6481 ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc
     6541 cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt
     6601 ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     6661 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6721 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6781 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6841 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6901 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6961 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     7021 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     7081 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7141 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     7201 ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7261 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7321 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7381 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7441 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7501 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7561 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7621 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7681 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7741 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc
     7801 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7861 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     7921 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7981 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8041 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8101 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8161 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8281 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8341 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8401 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8461 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     8521 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8581 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8641 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8701 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10621 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    10681 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    10741 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    10801 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    10861 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    10921 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
    10981 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    11041 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    11101 aaaacgacgg ccagtgccaa gctg
//
