LOCUS       V036815                10340 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036815
VERSION     V036815
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2296..2305
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2305..2370
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2371..2649
                     /codon_start=1
                     /gene=""
                     /gene_synonym=""
                     /note="binds synthetic ligands such as&nbsp;AP21967"
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /protein_id=""
                     /transl_table=1
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
                     /label="FRB*"
     CDS             2656..4545
                     /label="TRIM25(NM_005082)"
                     /note="TRIM25(NM_005082)"
                     /gene="TRIM25"
     CDS             4552..5262
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        5310..5521
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5534..5802
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5829..6428
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6435..7023
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7097..7330
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7402..7523
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7542..7677
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7715..7731)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7739..7755
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7763..7793)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7808..7829
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8117..8705)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8876..9736)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9737..9841)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10315..10331
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggactac aaagaccatg acggtgatta taaagatcat
     2341 gacatcgact acaaggatga cgatgacaag atcctctggc atgagatgtg gcatgaaggc
     2401 ctggaagagg catctcgttt gtactttggg gaaaggaacg tgaaaggcat gtttgaggtg
     2461 ctggagccct tgcatgctat gatggaacgg ggcccccaga ctctgaagga aacatccttt
     2521 aatcaggcct atggtcgaga tttaatggag gcccaagagt ggtgcaggaa gtacatgaaa
     2581 tcagggaatg tcaaggacct cctccaagcc tgggacctct attatcatgt gttccgacga
     2641 atctcaaagg gatccatggc agagctgtgc cccctggccg aggagctgtc gtgctccatc
     2701 tgcctggagc ccttcaagga gccggtcacc actccgtgcg gccacaactt ctgcgggtcg
     2761 tgcctgaatg agacgtgggc agtccagggc tcgccatacc tgtgcccgca gtgccgcgcc
     2821 gtctaccagg cgcgaccgca gctgcacaag aacacggtgc tgtgcaacgt ggtggagcag
     2881 ttcctgcagg ccgacctggc ccgggagcca cccgccgacg tctggacgcc gcccgcccgc
     2941 gcctctgcac ccagcccgaa tgcccaggtg gcctgcgacc actgcctgaa ggaggccgcc
     3001 gtgaagacgt gcttggtgtg catggcctcc ttctgtcagg agcacctgca gccgcacttc
     3061 gacagccccg ccttccagga ccacccgctg cagccgcccg ttcgcgacct gttgcgccgc
     3121 aaatgttccc agcacaatcg gctgcgggaa tttttctgcc ccgagcacag cgagtgcatc
     3181 tgccacatct gcctggtgga gcataagacc tgctctcccg cgtccctgag ccaggccagc
     3241 gccgacctgg aggccaccct gaggcacaaa ctaactgtca tgtacagtca gatcaacggg
     3301 gcgtcgagag cactggatga tgtgagaaac aggcagcagg atgtgcggat gactgcaaac
     3361 agaaaggtgg agcagctaca acaagaatac acggaaatga aggctctctt ggacgcctca
     3421 gagaccacct cgacaaggaa gataaaggaa gaggagaaga gggtcaacag caagtttgac
     3481 accatttatc agattctcct caagaagaag agtgagatcc agaccttgaa ggaggagatt
     3541 gaacagagcc tgaccaagag ggatgagttc gagtttctgg agaaagcatc aaaactgcga
     3601 ggaatctcaa caaagccagt ctacatcccc gaggtggaac tgaaccacaa gctgataaaa
     3661 ggcatccacc agagcaccat agacctcaaa aacgagctga agcagtgcat cgggcggctc
     3721 caggagccca cccccagttc aggtgaccct ggagagcatg acccagcgtc cacacacaaa
     3781 tccacacgcc ctgtgaagaa ggtctccaaa gaggaaaaga aatccaagaa acctccccct
     3841 gtccctgcct tacccagcaa gcttcccacg tttggagccc cggaacagtt agtggattta
     3901 aaacaagctg gcttggaggc tgcagccaaa gccaccagct cacatccgaa ctcaacatct
     3961 ctcaaggcca aggtgctgga gaccttcctg gccaagtcca gacctgagct cctggagtat
     4021 tacattaaag tcatcctgga ctacaacacc gcccacaaca aagtggctct gtcagagtgc
     4081 tatacagtag cttctgtggc tgagatgcct cagaactacc ggccgcatcc ccagaggttc
     4141 acatactgct ctcaggtgct gggcctgcac tgctacaaga aggggatcca ctactgggag
     4201 gtggagctgc agaagaacaa cttctgtggg gtaggcatct gctacggaag catgaaccgg
     4261 cagggcccag aaagcaggct cggccgcaac agcgcctcct ggtgcgtgga gtggttcaac
     4321 accaagatct ctgcctggca caataacgtg gagaaaaccc tgccctccac caaggccacg
     4381 cgggtgggcg tgcttctcaa ctgtgaccac ggctttgtca tcttcttcgc tgttgccgac
     4441 aaggtccacc tgatgtataa gttcagggtg gactttactg aggctttgta cccggctttc
     4501 tgggtatttt ctgctggtgc cacactctcc atctgctccc ccaagggatc catggtgagc
     4561 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     4621 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     4681 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     4741 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     4801 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     4861 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     4921 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     4981 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     5041 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     5101 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     5161 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     5221 ggccgccact ccaccggcgg catggacgag ctgtacaagt gaggatccgc ggccgcgaag
     5281 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     5341 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     5401 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     5461 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     5521 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     5581 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     5641 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     5701 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     5761 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     5821 tacgctagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     5881 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5941 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6001 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6061 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6121 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6181 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6241 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6301 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6361 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6421 gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6481 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6541 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6601 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6661 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6721 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6781 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6841 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6901 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6961 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7021 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7081 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     7141 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7201 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7261 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7321 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7381 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7441 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7501 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7561 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7621 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7681 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     7741 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7801 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7861 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7921 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7981 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8521 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9841 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9901 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     9961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    10021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    10081 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    10141 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    10201 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    10261 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    10321 cgacggccag tgccaagctg
//
