LOCUS       V034396                10183 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034396
VERSION     V034396
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5013
                     /label="TLR3(NM_003265)"
                     /note="TLR3(NM_003265)"
                     /gene="TLR3"
     CDS             5020..5109
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5153..5364
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5377..5645
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5672..6271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6278..6866
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6940..7173
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7245..7366
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7385..7520
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7558..7574)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7582..7598
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7606..7636)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7651..7672
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7960..8548)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8719..9579)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9580..9684)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10158..10174
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgagacag actttgcctt gtatctactt ttgggggggc
     2341 cttttgccct ttgggatgct gtgtgcatcc tccaccacca agtgcactgt tagccatgaa
     2401 gttgctgact gcagccacct gaagttgact caggtacccg atgatctacc cacaaacata
     2461 acagtgttga accttaccca taatcaactc agaagattac cagccgccaa cttcacaagg
     2521 tatagccagc taactagctt ggatgtagga tttaacacca tctcaaaact ggagccagaa
     2581 ttgtgccaga aacttcccat gttaaaagtt ttgaacctcc agcacaatga gctatctcaa
     2641 ctttctgata aaacctttgc cttctgcacg aatttgactg aactccatct catgtccaac
     2701 tcaatccaga aaattaaaaa taatcccttt gtcaagcaga agaatttaat cacattagat
     2761 ctgtctcata atggcttgtc atctacaaaa ttaggaactc aggttcagct ggaaaatctc
     2821 caagagcttc tattatcaaa caataaaatt caagcgctaa aaagtgaaga actggatatc
     2881 tttgccaatt catctttaaa aaaattagag ttgtcatcga atcaaattaa agagttttct
     2941 ccagggtgtt ttcacgcaat tggaagatta tttggcctct ttctgaacaa tgtccagctg
     3001 ggtcccagcc ttacagagaa gctatgtttg gaattagcaa acacaagcat tcggaatctg
     3061 tctctgagta acagccagct gtccaccacc agcaatacaa ctttcttggg actaaagtgg
     3121 acaaatctca ctatgctcga tctttcctac aacaacttaa atgtggttgg taacgattcc
     3181 tttgcttggc ttccacaact agaatatttc ttcctagagt ataataatat acagcatttg
     3241 ttttctcact ctttgcacgg gcttttcaat gtgaggtacc tgaatttgaa acggtctttt
     3301 actaaacaaa gtatttccct tgcctcactc cccaagattg atgatttttc ttttcagtgg
     3361 ctaaaatgtt tggagcacct taacatggaa gataatgata ttccaggcat aaaaagcaat
     3421 atgttcacag gattgataaa cctgaaatac ttaagtctat ccaactcctt tacaagtttg
     3481 cgaactttga caaatgaaac atttgtatca cttgctcatt ctcccttaca catactcaac
     3541 ctaaccaaga ataaaatctc aaaaatagag agtgatgctt tctcttggtt gggccaccta
     3601 gaagtacttg acctgggcct taatgaaatt gggcaagaac tcacaggcca ggaatggaga
     3661 ggtctagaaa atattttcga aatctatctt tcctacaaca agtacctgca gctgactagg
     3721 aactcctttg ccttggtccc aagccttcaa cgactgatgc tccgaagggt ggcccttaaa
     3781 aatgtggata gctctccttc accattccag cctcttcgta acttgaccat tctggatcta
     3841 agcaacaaca acatagccaa cataaatgat gacatgttgg agggtcttga gaaactagaa
     3901 attctcgatt tgcagcataa caacttagca cggctctgga aacacgcaaa ccctggtggt
     3961 cccatttatt tcctaaaggg tctgtctcac ctccacatcc ttaacttgga gtccaacggc
     4021 tttgacgaga tcccagttga ggtcttcaag gatttatttg aactaaagat catcgattta
     4081 ggattgaata atttaaacac acttccagca tctgtcttta ataatcaggt gtctctaaag
     4141 tcattgaacc ttcagaagaa tctcataaca tccgttgaga agaaggtttt cgggccagct
     4201 ttcaggaacc tgactgagtt agatatgcgc tttaatccct ttgattgcac gtgtgaaagt
     4261 attgcctggt ttgttaattg gattaacgag acccatacca acatccctga gctgtcaagc
     4321 cactaccttt gcaacactcc acctcactat catgggttcc cagtgagact ttttgataca
     4381 tcatcttgca aagacagtgc cccctttgaa ctctttttca tgatcaatac cagtatcctg
     4441 ttgattttta tctttattgt acttctcatc cactttgagg gctggaggat atctttttat
     4501 tggaatgttt cagtacatcg agttcttggt ttcaaagaaa tagacagaca gacagaacag
     4561 tttgaatatg cagcatatat aattcatgcc tataaagata aggattgggt ctgggaacat
     4621 ttctcttcaa tggaaaagga agaccaatct ctcaaatttt gtctggaaga aagggacttt
     4681 gaggcgggtg tttttgaact agaagcaatt gttaacagca tcaaaagaag cagaaaaatt
     4741 atttttgtta taacacacca tctattaaaa gacccattat gcaaaagatt caaggtacat
     4801 catgcagttc aacaagctat tgaacaaaat ctggattcca ttatattggt tttccttgag
     4861 gagattccag attataaact gaaccatgca ctctgtttgc gaagaggaat gtttaaatct
     4921 cactgcatct tgaactggcc agttcagaaa gaacggatag gtgcctttcg tcataaattg
     4981 caagtagcac ttggatccaa aaactctgta catggatccg aacaaaaact aatatcagag
     5041 gaagatttgg aacagaaact aatcagtgaa gaagatttag agcaaaagtt aatttccgag
     5101 gaggacttat agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     5161 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     5221 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     5281 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     5341 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     5401 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     5461 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5521 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     5581 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     5641 agatccaagc tgtgaccggc gcctacgcta gatgaccgag tacaagccca cggtgcgcct
     5701 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     5761 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     5821 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5881 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5941 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6001 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6061 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6121 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6181 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6241 ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat
     6301 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6361 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6421 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6481 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6541 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6601 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6661 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6721 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6781 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6841 gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag
     6901 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6961 aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7021 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7081 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7141 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7201 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     7261 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7321 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     7381 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7441 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7501 aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc
     7561 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7621 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7681 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7741 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7801 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7861 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7921 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7981 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8041 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8101 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8161 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8221 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8281 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8341 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8401 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8461 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8521 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8581 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8641 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8701 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8761 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8821 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8881 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8941 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9001 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9061 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9121 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9181 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9241 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9301 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9361 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9421 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9481 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9541 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9601 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9661 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9721 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     9781 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     9841 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     9901 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9961 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    10021 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10081 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    10141 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
