LOCUS       V028387                11013 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028387
VERSION     V028387
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2288..2297
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2297..2362
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2369..5938
                     /label="TJP2(NM_004817)"
                     /note="TJP2(NM_004817)"
                     /gene="TJP2"
     promoter        5983..6194
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6207..6475
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6502..7101
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7108..7696
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7770..8003
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8075..8196
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8215..8350
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8388..8404)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8412..8428
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8436..8466)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8481..8502
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8790..9378)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9549..10409)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10410..10514)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10988..11004
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc accatggact acaaagacca tgacggtgat tataaagatc atgacatcga
     2341 ctacaaggat gacgatgaca aggaattcat gccggtgcga ggagaccgcg ggtttccacc
     2401 ccggcgggag ctgtcaggtt ggctccgcgc cccaggcatg gaagagctga tatgggaaca
     2461 gtacactgtg accctacaaa aggattccaa aagaggattt ggaattgcag tgtccggagg
     2521 cagagacaac ccccactttg aaaatggaga aacgtcaatt gtcatttctg atgtgctccc
     2581 gggtgggcct gctgatgggc tgctccaaga aaatgacaga gtggtcatgg tcaatggcac
     2641 ccccatggag gatgtgcttc attcgtttgc agttcagcag ctcagaaaaa gtgggaaggt
     2701 cgctgctatt gtggtcaaga ggccccggaa ggtccaggtg gccgcacttc aggccagccc
     2761 tcccctggat caggatgacc gggcttttga ggtgatggac gagtttgatg gcagaagttt
     2821 ccggagtggc tacagcgaga ggagccggct gaacagccat ggggggcgca gccgcagctg
     2881 ggaggacagc ccggaaaggg ggcgtcccca tgagcgggcc cggagccggg agcgggacct
     2941 cagccgggac cggagccgtg gccggagcct ggagcggggc ctggaccaag accatgcgcg
     3001 cacccgagac cgcagccgtg gccggagcct ggagcggggc ctggaccacg actttgggcc
     3061 atcccgggac cgggaccgtg accgcagccg cggccggagc attgaccagg actacgagcg
     3121 agcctatcac cgggcctacg acccagacta cgagcgggcc tacagcccgg agtacaggcg
     3181 cggggcccgc cacgatgccc gctctcgggg accccgaagc cgcagccgcg agcacccgca
     3241 ctcacggagc cccagccccg agcctagggg gcggccgggg cccatcgggg tcctcctgat
     3301 gaaaagcaga gcgaacgaag agtatggtct ccggcttggg agtcagatct tcgtaaagga
     3361 aatgacccga acgggtctgg caactaaaga tggcaacctt cacgaaggag acataattct
     3421 caagatcaat gggactgtaa ctgagaacat gtctttaacg gatgctcgaa aattgataga
     3481 aaagtcaaga ggaaaactac agctagtggt gttgagagac agccagcaga ccctcatcaa
     3541 catcccgtca ttaaatgaca gtgactcaga aatagaagat atttcagaaa tagagtcaaa
     3601 ccgatcattt tctccagagg agagacgtca tcagtattct gattatgatt atcattcctc
     3661 aagtgagaag ctgaaggaaa ggccaagttc cagagaggac acgccgagca gattgtccag
     3721 gatgggtgcg acacccactc cctttaagtc cacaggggat attgcaggca cagttgtccc
     3781 agagaccaac aaggaaccca gataccaaga ggacccccca gctcctcaac caaaagcagc
     3841 cccgagaact tttcttcgtc ctagtcctga agatgaagca atatatggcc ctaataccaa
     3901 aatggtaagg ttcaagaagg gagacagcgt gggcctccgg ttggctggtg gcaatgatgt
     3961 cgggatattt gttgctggca ttcaagaagg gacctcggcg gagcaggagg gccttcaaga
     4021 aggagaccag attctgaagg tgaacacaca ggatttcaga ggattagtgc gggaggatgc
     4081 cgttctctac ctgttagaaa tccctaaagg tgaaatggtg accattttag ctcagagccg
     4141 agccgatgtg tatagagaca tcctggcttg tggcagaggg gattcgtttt ttataagaag
     4201 ccactttgaa tgtgagaagg aaactccaca gagcctggcc ttcaccagag gggaggtctt
     4261 ccgagtggta gacacactgt atgacggcaa gctgggcaac tggctggctg tgaggattgg
     4321 gaacgagttg gagaaaggct taatccccaa caagagcaga gctgaacaaa tggccagtgt
     4381 tcaaaatgcc cagagagaca acgctgggga ccgggcagat ttctggagaa tgcgtggcca
     4441 gaggtctggg gtgaagaaga acctgaggaa aagtcgggaa gacctcacag ctgttgtgtc
     4501 tgtcagcacc aagttcccag cttatgagag ggttttgctg cgagaagctg gtttcaagag
     4561 acctgtggtc ttattcggcc ccatagctga tatagcaatg gaaaaattgg ctaatgagtt
     4621 acctgactgg tttcaaactg ctaaaacgga accaaaagat gcaggatctg agaaatccac
     4681 tggagtggtc cggttaaata ccgtgaggca aattattgaa caggataagc atgcactact
     4741 ggatgtgact ccgaaagctg tggacctgtt gaattacacc cagtggttcc caattgtgat
     4801 ttttttcaac ccagactcca gacaaggtgt caaaaccatg agacaaaggt taaatccaac
     4861 gtccaacaaa agttctcgaa agttatttga tcaagccaac aagcttaaaa aaacgtgtgc
     4921 acaccttttt acagctacaa tcaacctaaa ttcagccaat gatagctggt ttggcagctt
     4981 aaaggacact attcagcatc agcaaggaga agcggtttgg gtctctgaag gaaagatgga
     5041 agggatggat gatgaccccg aagaccgcat gtcctactta accgccatgg gcgcggacta
     5101 tctgagttgc gacagccgcc tcatcagtga ctttgaagac acggacggtg aaggaggcgc
     5161 ctacactgac aatgagctgg atgagccagc cgaggagccg ctggtgtcgt ccatcacccg
     5221 ctcctcggag ccggtgcagc acgaggagag cataaggaaa cccagcccag agccacgagc
     5281 tcagatgagg agggctgcta gcagcgatca acttagggac aatagcccgc ccccagcatt
     5341 caagccagag ccgcccaagg ccaaaaccca gaacaaagaa gaatcctatg acttctccaa
     5401 atcctatgaa tataagtcaa acccctctgc cgttgctggt aatgaaactc ctggggcatc
     5461 taccaaaggt tatcctcctc ctgttgcagc aaaacctacc tttgggcggt ctatactgaa
     5521 gccctccact cccatccctc ctcaagaggg tgaggaggtg ggagagagca gtgaggagca
     5581 agataatgct cccaaatcag tcctgggcaa agtcaaaata tttgagaaga tggatcacaa
     5641 ggccaggtta cagagaatgc aggagctcca ggaagcacag aatgcaagga tcgaaattgc
     5701 ccagaagcat cctgatatct atgcagttcc aatcaaaacg cacaagccag accctggcac
     5761 gccccagcac acgagttcca gaccccctga gccacagaaa gctccttcca gaccttatca
     5821 ggataccaga ggaagttatg gcagtgatgc cgaggaggag gagtaccgcc agcagctgtc
     5881 agaacactcc aagcgcggtt actatggcca gtctgcccga taccgggaca cagaattata
     5941 ggcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     6001 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     6061 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     6121 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     6181 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     6241 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     6301 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     6361 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     6421 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     6481 tgtgaccggc gcctacgcta gatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6541 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6601 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6661 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6721 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6781 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6841 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6901 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6961 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7021 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7081 acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     7141 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7201 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7261 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7321 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7381 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7441 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7501 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7561 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7621 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7681 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7741 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata
     7801 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7861 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7921 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7981 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8041 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8161 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8341 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt
     8401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8581 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     9241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10501 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    10561 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    10621 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    10681 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10741 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    10801 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    10861 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10921 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10981 cgacgttgta aaacgacggc cagtgccaag ctg
//
