LOCUS       V031880                10981 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031880
VERSION     V031880
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5811
                     /label="THBS1(NM_003246)"
                     /note="THBS1(NM_003246)"
                     /gene="THBS1"
     CDS             5818..5907
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5951..6162
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6175..6443
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6470..7069
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7076..7664
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7738..7971
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8043..8164
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8183..8318
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8356..8372)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8380..8396
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8404..8434)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8449..8470
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8758..9346)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9517..10377)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10378..10482)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10956..10972
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggggctg gcctggggac taggcgtcct gttcctgatg
     2341 catgtgtgtg gcaccaaccg cattccagag tctggcggag acaacagcgt gtttgacatc
     2401 tttgaactca ccggggccgc ccgcaagggg tctgggcgcc gactggtgaa gggccccgac
     2461 ccttccagcc cagctttccg catcgaggat gccaacctga tcccccctgt gcctgatgac
     2521 aagttccaag acctggtgga tgctgtgcgg gcagaaaagg gtttcctcct tctggcatcc
     2581 ctgaggcaga tgaagaagac ccggggcacg ctgctggccc tggagcggaa agaccactct
     2641 ggccaggtct tcagcgtggt gtccaatggc aaggcgggca ccctggacct cagcctgacc
     2701 gtccaaggaa agcagcacgt ggtgtctgtg gaagaagctc tcctggcaac cggccagtgg
     2761 aagagcatca ccctgtttgt gcaggaagac agggcccagc tgtacatcga ctgtgaaaag
     2821 atggagaatg ctgagttgga cgtccccatc caaagcgtct tcaccagaga cctggccagc
     2881 atcgccagac tccgcatcgc aaaggggggc gtcaatgaca atttccaggg ggtgctgcag
     2941 aatgtgaggt ttgtctttgg aaccacacca gaagacatcc tcaggaacaa aggctgctcc
     3001 agctctacca gtgtcctcct cacccttgac aacaacgtgg tgaatggttc cagccctgcc
     3061 atccgcacta actacattgg ccacaagaca aaggacttgc aagccatctg cggcatctcc
     3121 tgtgatgagc tgtccagcat ggtcctggaa ctcaggggcc tgcgcaccat tgtgaccacg
     3181 ctgcaggaca gcatccgcaa agtgactgaa gagaacaaag agttggccaa tgagctgagg
     3241 cggcctcccc tatgctatca caacggagtt cagtacagaa ataacgagga atggactgtt
     3301 gatagctgca ctgagtgtca ctgtcagaac tcagttacca tctgcaaaaa ggtgtcctgc
     3361 cccatcatgc cctgctccaa tgccacagtt cctgatggag aatgctgtcc tcgctgttgg
     3421 cccagcgact ctgcggacga tggctggtct ccatggtccg agtggacctc ctgttctacg
     3481 agctgtggca atggaattca gcagcgcggc cgctcctgcg atagcctcaa caaccgatgt
     3541 gagggctcct cggtccagac acggacctgc cacattcagg agtgtgacaa gagatttaaa
     3601 caggatggtg gctggagcca ctggtccccg tggtcatctt gttctgtgac atgtggtgat
     3661 ggtgtgatca caaggatccg gctctgcaac tctcccagcc cccagatgaa cgggaaaccc
     3721 tgtgaaggcg aagcgcggga gaccaaagcc tgcaagaaag acgcctgccc catcaatgga
     3781 ggctggggtc cttggtcacc atgggacatc tgttctgtca cctgtggagg aggggtacag
     3841 aaacgtagtc gtctctgcaa caaccccaca ccccagtttg gaggcaagga ctgcgttggt
     3901 gatgtaacag aaaaccagat ctgcaacaag caggactgtc caattgatgg atgcctgtcc
     3961 aatccctgct ttgccggcgt gaagtgtact agctaccctg atggcagctg gaaatgtggt
     4021 gcttgtcccc ctggttacag tggaaatggc atccagtgca cagatgttga tgagtgcaaa
     4081 gaagtgcctg atgcctgctt caaccacaat ggagagcacc ggtgtgagaa cacggacccc
     4141 ggctacaact gcctgccctg ccccccacgc ttcaccggct cacagccctt cggccagggt
     4201 gtcgaacatg ccacggccaa caaacaggtg tgcaagcccc gtaacccctg cacggatggg
     4261 acccacgact gcaacaagaa cgccaagtgc aactacctgg gccactatag cgaccccatg
     4321 taccgctgcg agtgcaagcc tggctacgct ggcaatggca tcatctgcgg ggaggacaca
     4381 gacctggatg gctggcccaa tgagaacctg gtgtgcgtgg ccaatgcgac ttaccactgc
     4441 aaaaaggata attgccccaa ccttcccaac tcagggcagg aagactatga caaggatgga
     4501 attggtgatg cctgtgatga tgacgatgac aatgataaaa ttccagatga cagggacaac
     4561 tgtccattcc attacaaccc agctcagtat gactatgaca gagatgatgt gggagaccgc
     4621 tgtgacaact gtccctacaa ccacaaccca gatcaggcag acacagacaa caatggggaa
     4681 ggagacgcct gtgctgcaga cattgatgga gacggtatcc tcaatgaacg ggacaactgc
     4741 cagtacgtct acaatgtgga ccagagagac actgatatgg atggggttgg agatcagtgt
     4801 gacaattgcc ccttggaaca caatccggat cagctggact ctgactcaga ccgcattgga
     4861 gatacctgtg acaacaatca ggatattgat gaagatggcc accagaacaa tctggacaac
     4921 tgtccctatg tgcccaatgc caaccaggct gaccatgaca aagatggcaa gggagatgcc
     4981 tgtgaccacg atgatgacaa cgatggcatt cctgatgaca aggacaactg cagactcgtg
     5041 cccaatcccg accagaagga ctctgacggc gatggtcgag gtgatgcctg caaagatgat
     5101 tttgaccatg acagtgtgcc agacatcgat gacatctgtc ctgagaatgt tgacatcagt
     5161 gagaccgatt tccgccgatt ccagatgatt cctctggacc ccaaagggac atcccaaaat
     5221 gaccctaact gggttgtacg ccatcagggt aaagaactcg tccagactgt caactgtgat
     5281 cctggactcg ctgtaggtta tgatgagttt aatgctgtgg acttcagtgg caccttcttc
     5341 atcaacaccg aaagggacga tgactatgct ggatttgtct ttggctacca gtccagcagc
     5401 cgcttttatg ttgtgatgtg gaagcaagtc acccagtcct actgggacac caaccccacg
     5461 agggctcagg gatactcggg cctttctgtg aaagttgtaa actccaccac agggcctggc
     5521 gagcacctgc ggaacgccct gtggcacaca ggaaacaccc ctggccaggt gcgcaccctg
     5581 tggcatgacc ctcgtcacat aggctggaaa gatttcaccg cctacagatg gcgtctcagc
     5641 cacaggccaa agacgggttt cattagagtg gtgatgtatg aagggaagaa aatcatggct
     5701 gactcaggac ccatctatga taaaacctat gctggtggta gactagggtt gtttgtcttc
     5761 tctcaagaaa tggtgttctt ctctgacctg aaatacgaat gtagagatcc cggatccgaa
     5821 caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga agatttagag
     5881 caaaagttaa tttccgagga ggacttatag cggccgcgaa ggatctgcga tcgctccggt
     5941 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6001 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6061 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6121 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     6181 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     6241 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     6301 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     6361 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6421 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta
     6481 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     6541 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     6601 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6661 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6721 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6781 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6841 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6901 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6961 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     7021 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca
     7081 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7141 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7201 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7261 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7321 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7381 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7441 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     7501 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7561 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7621 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac
     7681 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7741 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     7801 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7861 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7921 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7981 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8041 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8101 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8161 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     8221 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8281 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     8341 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8401 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8461 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8521 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8941 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9181 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9601 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9661 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9721 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9781 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9841 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9901 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9961 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10021 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10081 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10141 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10201 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10261 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10321 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10381 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10441 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10501 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10561 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10621 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10681 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10741 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10801 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10861 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10921 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    10981 g
//
