LOCUS V045870 9665 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045870 VERSION V045870 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..3666 /label="TFAP2C(NM_003222)" /note="TFAP2C(NM_003222)" /gene="TFAP2C" CDS 3673..3699 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3703..3729 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3733..3759 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3824..4035 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4048..4316 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 4345..4354 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4375..5037 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5107..5160 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5161..5760 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5761..6348 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6422..6655 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6727..6848 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6867..7002 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7040..7056) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7064..7080 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7088..7118) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7133..7154 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7442..8030) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8201..9061) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9062..9166) /gene="bla" /label="AmpR promoter" primer_bind 9640..9656 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tgtggaaaat aaccgataat 2341 gtcaagtacg aagaggactg cgaggatcgc cacgacggga gcagcaatgg gaatccgcgg 2401 gtcccccacc tctcctccgc cgggcagcac ctctacagcc ccgcgccacc cctctcccac 2461 actggagtcg ccgaatatca gccgccaccc tactttcccc ctccctacca gcagctggcc 2521 tactcccagt cggccgaccc ctactcgcat ctgggggaag cgtacgccgc cgccatcaac 2581 cccctgcacc agccggcgcc cacaggcagc cagcagcagg cctggcccgg ccgccagagc 2641 caggagggag cggggctgcc ctcgcaccac gggcgcccgg ccggcctact gccccacctc 2701 tccgggctgg aggcgggcgc ggtgagcgcc cgcagggatg cctaccgccg ctccgacctg 2761 ctgctgcccc acgcacacgc cctggatgcc gcgggcctgg ccgagaacct ggggctccac 2821 gacatgcctc accagatgga cgaggtgcag aatgtcgacg accagcacct gttgctgcac 2881 gatcagacag tcattcgcaa aggtcccatt tccatgacca agaaccctct gaacctcccc 2941 tgtcagaagg agctggtggg ggccgtaatg aaccccactg aggtcttctg ctcagtccct 3001 ggaagattgt cgctcctcag ctctacgtct aaatacaaag tgacagtggc tgaagtacag 3061 aggcgactgt ccccacctga atgcttaaat gcctcgttac tgggaggtgt tctcagaaga 3121 gccaaatcga aaaatggagg ccggtccttg cgggagaagt tggacaagat tgggttgaat 3181 cttccggccg ggaggcggaa agccgctcat gtgactctcc tgacatcctt agtagaaggt 3241 gaagctgttc atttggctag ggactttgcc tatgtctgtg aagccgaatt tcctagtaaa 3301 ccagtggcag aatatttaac cagacctcat cttggaggac gaaatgagat ggcagctagg 3361 aagaacatgc tattggcggc ccagcaactg tgtaaagaat tcacagaact tctcagccaa 3421 gaccggacac cccatgggac cagcaggctc gccccagtct tggagacgaa catacagaac 3481 tgcttgtctc atttcagcct gattacccac gggtttggca gccaggccat ctgtgccgcg 3541 gtgtctgccc tgcagaacta catcaaagaa gccctgattg tcatagacaa atcctacatg 3601 aaccctggag accagagtcc agctgattct aacaaaaccc tggagaaaat ggagaaacac 3661 aggaaactcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct 3721 gattatgcta gctatcctta tgatgtccca gactacgcat agggatccct gcgatatcca 3781 gcgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3841 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 3901 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 3961 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4021 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4081 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4141 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4201 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4261 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4321 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4381 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4441 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4501 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4561 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4621 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4681 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4741 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 4801 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 4861 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 4921 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 4981 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5041 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5101 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5161 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5221 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5281 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5341 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5401 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5461 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5521 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5581 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5641 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5701 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5761 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5821 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5881 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 5941 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6001 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6061 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6121 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 6181 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6241 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 6301 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 6361 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 6421 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 6481 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6541 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6601 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6661 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 6721 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 6781 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 6841 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 6901 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 6961 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 7021 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 7081 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 7141 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 7201 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 7261 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7321 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7381 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7441 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7501 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7561 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7621 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7681 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7741 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7801 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7861 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 7921 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7981 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 8041 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 8101 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 8161 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 8221 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 8281 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 8341 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8401 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8461 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8521 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8581 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8641 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8701 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8761 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8821 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8881 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8941 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9001 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 9061 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 9121 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 9181 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 9241 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 9301 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 9361 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 9421 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 9481 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9541 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 9601 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 9661 agctg //