LOCUS       V024986                10842 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024986
VERSION     V024986
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..5696
                     /EC_number=""
                     /codon_start=1
                     /gene="TERT"
                     /note="prevents telomere shortening and allows
                     postsenescent cells to proliferate beyond crisis (Counter
                     et al., 1998); also known as EST2"
                     /product="human telomerase reverse transcriptase, the
                     catalytic subunit of telomerase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
                     /label="hTERT"
     CDS             5703..5768
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5812..6023
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6036..6304
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6331..6930
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6937..7525
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7599..7832
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7904..8025
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8044..8179
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8217..8233)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8241..8257
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8265..8295)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8310..8331
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8619..9207)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9378..10238)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10239..10343)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10817..10833
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atgccgcgcg ctccccgctg ccgagccgtg cgctccctgc
     2341 tgcgcagcca ctaccgcgag gtgctgccgc tggccacgtt cgtgcggcgc ctggggcccc
     2401 agggctggcg gctggtgcag cgcggggacc cggcggcttt ccgcgcgctg gtggcccagt
     2461 gcctggtgtg cgtgccctgg gacgcacggc cgccccccgc cgccccctcc ttccgccagg
     2521 tgtcctgcct gaaggagctg gtggcccgag tgctgcagag gctgtgcgag cgcggcgcga
     2581 agaacgtgct ggccttcggc ttcgcgctgc tggacggggc ccgcgggggc ccccccgagg
     2641 ccttcaccac cagcgtgcgc agctacctgc ccaacacggt gaccgacgca ctgcggggga
     2701 gcggggcgtg ggggctgctg ctgcgccgcg tgggcgacga cgtgctggtt cacctgctgg
     2761 cacgctgcgc gctctttgtg ctggtggctc ccagctgcgc ctaccaggtg tgcgggccgc
     2821 cgctgtacca gctcggcgct gccactcagg cccggccccc gccacacgct agtggacccc
     2881 gaaggcgtct gggatgcgaa cgggcctgga accatagcgt cagggaggcc ggggtccccc
     2941 tgggcctgcc agccccgggt gcgaggaggc gcgggggcag tgccagccga agtctgccgt
     3001 tgcccaagag gcccaggcgt ggcgctgccc ctgagccgga gcggacgccc gttgggcagg
     3061 ggtcctgggc ccacccgggc aggacgcgtg gaccgagtga ccgtggtttc tgtgtggtgt
     3121 cacctgccag acccgccgaa gaagccacct ctttggaggg tgcgctctct ggcacgcgcc
     3181 actcccaccc atccgtgggc cgccagcacc acgcgggccc cccatccaca tcgcggccac
     3241 cacgtccctg ggacacgcct tgtcccccgg tgtacgccga gaccaagcac ttcctctact
     3301 cctcaggcga caaggagcag ctgcggccct ccttcctact cagctctctg aggcccagcc
     3361 tgactggcgc tcggaggctc gtggagacca tctttctggg ttccaggccc tggatgccag
     3421 ggactccccg caggttgccc cgcctgcccc agcgctactg gcaaatgcgg cccctgtttc
     3481 tggagctgct tgggaaccac gcgcagtgcc cctacggggt gctcctcaag acgcactgcc
     3541 cgctgcgagc tgcggtcacc ccagcagccg gtgtctgtgc ccgggagaag ccccagggct
     3601 ctgtggcggc ccccgaggag gaggacacag acccccgtcg cctggtgcag ctgctccgcc
     3661 agcacagcag cccctggcag gtgtacggct tcgtgcgggc ctgcctgcgc cggctggtgc
     3721 ccccaggcct ctggggctcc aggcacaacg aacgccgctt cctcaggaac accaagaagt
     3781 tcatctccct ggggaagcat gccaagctct cgctgcagga gctgacgtgg aagatgagcg
     3841 tgcgggactg cgcttggctg cgcaggagcc caggggttgg ctgtgttccg gccgcagagc
     3901 accgtctgcg tgaggagatc ctggccaagt tcctgcactg gctgatgagt gtgtacgtcg
     3961 tcgagctgct caggtctttc ttttatgtca cggagaccac gtttcaaaag aacaggctct
     4021 ttttctaccg gaagagtgtc tggagcaagt tgcaaagcat tggaatcaga cagcacttga
     4081 agagggtgca gctgcgggag ctgtcggaag cagaggtcag gcagcatcgg gaagccaggc
     4141 ccgccctgct gacgtccaga ctccgcttca tccccaagcc tgacgggctg cggccgattg
     4201 tgaacatgga ctacgtcgtg ggagccagaa cgttccgcag agaaaagagg gccgagcgtc
     4261 tcacctcgag ggtgaaggca ctgttcagcg tgctcaacta cgagcgggcg cggcgccccg
     4321 gcctcctggg cgcctctgtg ctgggcctgg acgatatcca cagggcctgg cgcaccttcg
     4381 tgctgcgtgt gcgggcccag gacccgccgc ctgagctgta ctttgtcaag gtggatgtga
     4441 cgggcgcgta cgacaccatc ccccaggaca ggctcacgga ggtcatcgcc agcatcatca
     4501 aaccccagaa cacgtactgc gtgcgtcggt atgccgtggt ccagaaggcc gcccatgggc
     4561 acgtccgcaa ggccttcaag agccacgtct ctaccttgac agacctccag ccgtacatgc
     4621 gacagttcgt ggctcacctg caggagacca gcccgctgag ggatgccgtc gtcatcgagc
     4681 agagctcctc cctgaatgag gccagcagtg gcctcttcga cgtcttccta cgcttcatgt
     4741 gccaccacgc cgtgcgcatc aggggcaagt cctacgtcca gtgccagggg atcccgcagg
     4801 gctccatcct ctccacgctg ctctgcagcc tgtgctacgg cgacatggag aacaagctgt
     4861 ttgcggggat tcggcgggac gggctgctcc tgcgtttggt ggatgatttc ttgttggtga
     4921 cacctcacct cacccacgcg aaaaccttcc tcaggaccct ggtccgaggt gtccctgagt
     4981 atggctgcgt ggtgaacttg cggaagacag tggtgaactt ccctgtagaa gacgaggccc
     5041 tgggtggcac ggcttttgtt cagatgccgg cccacggcct attcccctgg tgcggcctgc
     5101 tgctggatac ccggaccctg gaggtgcaga gcgactactc cagctatgcc cggacctcca
     5161 tcagagccag tctcaccttc aaccgcggct tcaaggctgg gaggaacatg cgtcgcaaac
     5221 tctttggggt cttgcggctg aagtgtcaca gcctgtttct ggatttgcag gtgaacagcc
     5281 tccagacggt gtgcaccaac atctacaaga tcctcctgct gcaggcgtac aggtttcacg
     5341 catgtgtgct gcagctccca tttcatcagc aagtttggaa gaaccccaca tttttcctgc
     5401 gcgtcatctc tgacacggcc tccctctgct actccatcct gaaagccaag aacgcaggga
     5461 tgtcgctggg ggccaagggc gccgccggcc ctctgccctc cgaggccgtg cagtggctgt
     5521 gccaccaagc attcctgctc aagctgactc gacaccgtgt cacctacgtg ccactcctgg
     5581 ggtcactcag gacagcccag acgcagctga gtcggaagct cccggggacg acgctgactg
     5641 ccctggaggc cgcagccaac ccggcactgc cctcagactt caagaccatc ctggacggat
     5701 ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
     5761 atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc
     5821 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct
     5881 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     5941 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     6001 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg
     6061 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg
     6121 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac
     6181 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt
     6241 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca
     6301 gatccaagct gtgaccggcg cctacgctag atgaccgagt acaagcccac ggtgcgcctc
     6361 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6421 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     6481 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6541 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6601 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6661 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6721 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6781 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6841 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6901 tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga ttacaaaatt
     6961 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7021 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7081 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7141 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7201 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7261 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7321 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7381 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7441 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7501 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     7561 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7621 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7681 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7741 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7801 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7861 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7921 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7981 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8041 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8101 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8161 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     8221 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8281 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8341 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8401 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9361 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9421 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9481 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9541 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9601 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9661 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9721 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9781 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9841 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9901 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9961 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10021 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10081 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10141 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10201 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10261 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10321 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    10381 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    10441 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    10501 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    10561 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    10621 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    10681 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    10741 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    10801 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
