LOCUS       V022858                11926 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022858
VERSION     V022858
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6021
                     /label="TAOK2(NM_016151)"
                     /note="TAOK2(NM_016151)"
                     /gene="TAOK2"
     promoter        6085..6296
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6309..6577
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6606..6615
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6636..7298
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7368..7421
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7422..8021
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8022..8609
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8683..8916
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8988..9109
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9128..9263
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9301..9317)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9325..9341
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9349..9379)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9394..9415
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9703..10291)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10462..11322)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11323..11427)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11901..11917
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cagctggggg ccgggccggg
     2341 agcctgaagg acccagatgt ggctgagctc ttcttcaagg atgacccaga aaagctcttc
     2401 tctgacctcc gggaaattgg ccatggcagc tttggagccg tatactttgc ccgggatgtc
     2461 cggaatagtg aggtggtggc catcaagaag atgtcctaca gtgggaagca gtccaatgag
     2521 aaatggcaag acatcatcaa ggaggtgcgg ttcttacaga agctccggca tcccaacacc
     2581 attcagtacc ggggctgtta cctgagggag cacacggctt ggctggtaat ggagtattgc
     2641 ctgggctcag cttctgacct tctagaagtg cacaagaaac cccttcagga ggtagagatc
     2701 gcagctgtga cccacggggc gcttcagggc ctggcatatc tgcactccca caacatgatc
     2761 catagggatg tgaaggctgg aaacatcctg ctgtcagagc cagggttagt gaagctaggg
     2821 gactttggtt ctgcgtccat catggcacct gccaactcct tcgtgggcac cccatactgg
     2881 atggcacccg aggtgatcct ggccatggat gaggggcagt acgatggcaa agtggacgtc
     2941 tggtccttgg ggataacctg catcgagctg gctgaacgga aaccaccgct ctttaacatg
     3001 aatgcgatga gtgccttata ccacattgca cagaacgaat cccccgtgct ccagtcagga
     3061 cactggtctg agtacttccg gaattttgtc gactcctgtc ttcagaaaat ccctcaagac
     3121 agaccaacct cagaggttct cctgaagcac cgctttgtgc tccgggagcg gccacccaca
     3181 gtcatcatgg acctgatcca gaggaccaag gatgccgtgc gggagctgga caacctgcag
     3241 taccgcaaga tgaagaagat cctgttccaa gaggcaccca acggccctgg tgccgaggcc
     3301 ccagaggagg aagaggaggc cgagccctac atgcaccggg ccgggactct gaccagcctc
     3361 gagagtagcc actcagtgcc cagcatgtcc atcagcgcct ccagccagag cagctccgtc
     3421 aacagcctag cagatgcctc agacaacgag gaagaggagg aggaggagga ggaagaggag
     3481 gaggaggaag aaggccctga agcccgggag atggccatga tgcaggaggg ggagcacaca
     3541 gtcacctctc acagctccat tatccaccgg ctgccgggct ctgacaacct atatgatgac
     3601 ccctaccagc cagagataac ccccagccct ctccagccgc ctgcagcccc agctcccact
     3661 tccaccacct cttccgcccg ccgccgggcc tactgccgta accgagacca ctttgccacc
     3721 atccgaaccg cctccctggt cagccgtcag atccaggagc atgagcagga ctctgcgctg
     3781 cgggagcagc tgagcggcta taagcggatg cgacgacagc accagaagca gctgctggcc
     3841 ctggagtcac ggctgagggg tgaacgggag gagcacagtg cacggctgca gcgggagctt
     3901 gaggcgcagc gggctggctt tggggcagag gcagaaaagc tggcccggcg gcaccaggcc
     3961 ataggtgaga aggaggcacg agctgcccag gccgaggagc ggaagttcca gcagcacatc
     4021 cttgggcagc agaagaagga gctggctgcc ctgctggagg cacagaagcg gacctacaaa
     4081 cttcgcaagg aacagctgaa ggaggagctc caggagaacc ccagcactcc caagcgggag
     4141 aaggccgagt ggctgctgcg gcagaaggag cagctccagc agtgccaggc ggaggaggaa
     4201 gcagggctgc tgcggcggca gcgccagtac tttgagctgc agtgtcgcca gtacaagcgc
     4261 aagatgttgc tggctcggca cagcctggac caggacctgc tgcgggagga cctgaacaag
     4321 aagcagaccc agaaggactt ggagtgtgca ctgctgcttc ggcagcacga ggccacgcgg
     4381 gagctggagc tgcggcagct ccaggccgtg cagcgcacgc gggctgagct cacccgcctg
     4441 cagcaccaga cggagctggg caaccagctg gagtacaaca agcggcgtga gcaagagttg
     4501 cggcagaagc atgcggccca ggttcgccag cagcccaaga gcctcaaagt acgtgcaggc
     4561 cagcgccccc cgggccttcc actccccatt cctggggctc tgggcccacc caacacaggc
     4621 acccctatag aacagcagcc ctgctcacct ggccaggagg cagtcctgga ccaaagaatg
     4681 cttggcgagg aggaggaagc agttggagag agaaggattc tgggaaagga aggggccact
     4741 ttggagccca agcagcagag gattctgggg gaagaatcag gagcccctag tcccagtcca
     4801 caaaaacatg ggagcctggt tgatgaggaa gtttggggtc tgcctgagga gatagaggag
     4861 cttagggtgc cctcccttgt accccaggag aggagcattg ttggccagga ggaggctggg
     4921 acatggagct tgtgggggaa ggaggatgag agtcttctgg atgaggagtt tgagcttggc
     4981 tgggtccagg gcccagcact gactcccgtc cctgaggagg aggaagaaga ggaagagggg
     5041 gctccgattg ggacccctag ggatcctgga gatggttgtc cttcccccga catccctcct
     5101 gaaccccctc caacacacct gaggccctgc cctgccagcc agctccctgg actcctgtcc
     5161 catggcctcc tggccggcct ctcctttgca gtggggtcct cctctggcct cctgcccctc
     5221 ctgctgctgc tgctgcttcc attgctggca gcccagggtg ggggtggcct gcaggcagcg
     5281 ctgctggccc ttgaggtggg gctggtgggt ctgggggcct cctacctgct cctttgtaca
     5341 gccctgcacc tgccctccag tcttttccta ctcctggccc agggtaccgc actgggggcc
     5401 gtcctgggcc tgagctggcg ccgaggcctc atgggtgttc ccctgggcct tggagctgcc
     5461 tggctcttag cttggccagg cctagctcta cctctggtgg ctatggcagc ggggggcaga
     5521 tgggtgcggc agcagggccc ccgggtgcgc cggggcatat ctcgactctg gttgcgggtt
     5581 ctgctgcgcc tgtcacccat ggccttccgg gccctgcagg gctgtggggc tgtgggggac
     5641 cggggtctgt ttgcactgta ccccaaaacc aacaaggatg gcttccgcag ccgcctgccc
     5701 gtccctgggc cccggcggcg taatccccgc accacccaac acccattagc tctgttggca
     5761 agggtctggg tcctgtgcaa gggctggaac tggcgtctgg cacgggccag ccagggttta
     5821 gcatcccact tgcccccgtg ggccatccac acactggcca gctggggcct gcttcggggt
     5881 gaacggccca cccgaatccc ccggctacta ccacgcagcc agcgccagct agggccccct
     5941 gcctcccgcc agccactgcc agggactcta gccgggcgga ggtcacgcac ccgccagtcc
     6001 cgggccctgc ccccctggag gtaggatccc tgcgatatcc agcgcggccg cgaaggatct
     6061 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     6121 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
     6181 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     6241 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga
     6301 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc
     6361 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg
     6421 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga
     6481 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg
     6541 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc
     6601 tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca
     6661 tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca
     6721 agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct
     6781 acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct
     6841 acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga
     6901 agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg
     6961 tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg
     7021 acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc
     7081 tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg
     7141 tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc
     7201 ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg
     7261 agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca
     7321 attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa
     7381 gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca
     7441 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     7501 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     7561 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     7621 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     7681 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     7741 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     7801 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     7861 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7921 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     7981 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca
     8041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     8101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     8161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     8221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     8461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8521 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga
     8581 gtcggatctc cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     8641 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     8701 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8761 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8821 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8881 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     8941 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     9001 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9061 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     9121 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9181 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9241 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     9301 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9361 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9421 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10141 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10201 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10261 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10321 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10381 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10441 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10501 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10561 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10621 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10681 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10741 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10801 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10861 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10921 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10981 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11041 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11101 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11161 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11221 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11281 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11341 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11401 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11461 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11521 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11581 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11641 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11701 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11761 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11821 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11881 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
