LOCUS       V047273                10566 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047273
VERSION     V047273
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4648
                     /label="Sp4(NM_009239)"
                     /note="Sp4(NM_009239)"
                     /gene="Sp4"
     CDS             4655..4720
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4764..4975
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4988..5256
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5291..5998
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6008..6061
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6062..6661
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6662..7249
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7323..7556
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7628..7749
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7768..7903
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7941..7957)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7965..7981
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7989..8019)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8034..8055
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8343..8931)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9102..9962)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9963..10067)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10541..10557
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgagcga tcagaagaag gaggaggagg aggaggcggc
     2341 agcggcgatg gctacagaag gagggaaaac ctctgagcca gagaataaca ataaaaaacc
     2401 caaaacctca ggctcccagg actctcagcc ctctcctctg gctttactgg cagctacttg
     2461 cagcaaaata gggactcctg gtgaaaatca agcaactgga caacagcaga ttattataga
     2521 tccaagccaa ggattggtgc aacttcagaa tcagccacag cagctagaac tggtgacgac
     2581 gcagcttgct ggaaacgctt ggcaacttgt tgcctccact cctcctgctt caaaagaaaa
     2641 taacgtttct cagccagctt ctagttcatc tagttcttcc agcagtaata acgggagttc
     2701 ctctcctaca aaaactaaat caggtaatcc ttccacccct aatcaatttc aagtcataca
     2761 agtacaaaac ccaagcggta gtgtacagta ccaagtgatc ccccaacttc agacggtgga
     2821 aggccagcaa attcaaatca acccaactag tagttcatct ctacaggatt tgcagggtca
     2881 aattcagctc atttctgcag gtaataatca agctatcctc acggctgcta acaggacagc
     2941 ttctgggaat atccttgctc aaaacctggc aaatcagaca gttccagtac aaattagacc
     3001 tggcgtttca ataccattgc aattgcagac ccttcctggt actcaggctc aagttgtaac
     3061 aaccctacca attaacattg gaggagtgac tttagctttg ccagtaataa ataacgtgac
     3121 tgctggagga gggactggac aagtcggcca gcctactact actactgaca gtgggacttc
     3181 caatgggaat cagttagttt ctacgcccac caccagcact gctcccgcca gtactatgcc
     3241 tgaatcgccc tcctcctcta ctacctgcac aaccaccgca tcaacaactc tgacgagcag
     3301 tgacacatta gtgagctcag cagatacagg ccagtatgca agcacatcag ccagtagttc
     3361 tgaacgcacc attgaagaac cacagacacc tgctgctact gagtctgaag cccagagctc
     3421 cagccagctt cagtccaacg gaattcagaa tgcacaagac cagtcaaatt cccttcaaca
     3481 agtacagatt gtaggccagc ctatcctgca gcaaatccag atccagcagc ctcagcagca
     3541 gattattcaa gctatcccac cacagtcatt tcagctccag tcagggcaga caattcagac
     3601 catccagcag cagcctttgc agaatgttca acttcaagca gtaaacccga ctcaggtact
     3661 tatcagggct ccaactttaa caccttcagg gcaaatcagc tggcaaactg tccaggtcca
     3721 gaatattcaa agtctttcaa atttgcaagt tcagaatgct gggttatccc agcagttaac
     3781 catcacccca gtgtcttcaa gtggtggcac aactcttgct cagattgctc ctgtggctgt
     3841 tgctggtgcc ccaataactt tgaatactgc ccagcttgca tcagtgccta accttcagac
     3901 agtgagcgtt gccaacctgg gtgctgcggg tgttcaagtt cagggagttc cagtaacaat
     3961 cactagtgtt gcaggtcaac agcaaggaca ggatggagtg aaagtccaac aagctactat
     4021 agctcctgta acggtggcag ttggagggat tgctaatgcg acaataggtg ctgttagtcc
     4081 tgaccaactc acacaagtgc atttgcagca aggccagcag acctctgatg cagaggtgca
     4141 acctggcaag aggctccgaa gagttgcctg ctcctgtcct aactgtaggg aaggagaagg
     4201 aaggggcagc agtgaaccag gaaagaagaa gcagcatgtt tgccacattg aagggtgtgg
     4261 gaaagtatat ggcaagacct ctcacctccg agcacatctc cgatggcaca ctggagaaag
     4321 accgttcata tgcaactgga tgttttgtgg caaaagattc acaaggagcg atgagcttca
     4381 gagacacaga aggacccaca caggtgaaaa gagatttgaa tgtccagagt gttctaaaag
     4441 gtttatgcgg agtgatcacc tatccaagca tgtcaaaact caccagaata agaaaggagg
     4501 tgggacagct cttgccattg ttacctccgg agaactggac tcatctgtta cagaggtgct
     4561 tggctctcca cggattgtca cggttgccgc catttcccaa gattcgaatc cagcaactcc
     4621 caatgtttca acaaacatgg aagaattcgg atccgactac aaagaccatg acggtgatta
     4681 taaagatcat gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg
     4741 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4801 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     4861 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4921 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     4981 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     5041 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     5101 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     5161 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     5221 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     5281 agacgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     5341 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     5401 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     5461 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     5521 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     5581 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     5641 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     5701 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     5761 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     5821 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     5881 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     5941 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg
     6001 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     6061 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6361 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6661 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6721 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6781 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6841 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6901 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6961 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7021 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7081 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7141 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7201 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt
     7261 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7321 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     7381 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7441 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7501 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7561 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7621 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7741 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     7801 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7861 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     7921 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7981 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8041 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8101 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8161 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8221 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8761 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10081 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    10141 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    10201 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    10261 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    10321 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10381 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    10441 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    10501 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    10561 aagctg
//
