LOCUS       V036577                10153 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036577
VERSION     V036577
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4179
                     /label="Slc6a6(NM_009320)"
                     /note="Slc6a6(NM_009320)"
                     /gene="Slc6a6"
     CDS             4186..4251
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4295..4506
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4519..4787
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4816..4825
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4846..5508
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5578..5631
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5632..6231
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6232..6820
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6894..7127
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7199..7320
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7360..7495
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7528..7544)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7552..7568
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7576..7606)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7621..7642
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7930..8518)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8689..9549)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9550..9654)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10128..10144
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccacgaagga gaagctgcaa
     2341 tgtctgaaag acttccacaa agacatcctg aagccttctc cagggaagag cccaggcaca
     2401 cggcctgaag atgaggcgga cgggaagccc cctcagaggg agaagtggtc cagcaagatc
     2461 gactttgtgc tgtctgtggc cggaggcttc gtgggtttgg gcaacgtctg gcgtttcccg
     2521 tacctctgct acaaaaatgg tggaggtgcg ttcctcatac cgtattttat tttcctgttt
     2581 gggagcggcc tgcctgtgtt tttcttggag gtcatcatag gccagtacac atcagaaggg
     2641 ggcatcacct gctgggagaa gatctgtcct ttgttctctg gcattggcta cgcatccatc
     2701 gtcattgtgt ccctcctgaa cgtgtactac atcgtcatcc tggcctgggc cacatactac
     2761 ctattccact ctttccagaa ggatcttccc tgggcccact gcaaccatag ctggaacaca
     2821 ccacagtgca tggaggacac cctgcgtagg aacgagagtc actgggtctc ccttagcact
     2881 gccaacttca cctcacccgt catcgagttc tgggagcgca atgtgctcag cctgtcctcc
     2941 ggaatcgaca acccaggcag tctgaaatgg gacctcgcgc tctgcctcct cttagtctgg
     3001 ctcgtctgtt ttttctgcat ctggaagggt gttcgatcca caggcaaggt tgtctacttc
     3061 accgctactt tcccgtttgc catgcttctg gtgctgctgg tccgtggact gaccctgcca
     3121 ggtgctggtg aaggcatcaa attctacctg taccctgaca tcagccgcct tggggaccca
     3181 caggtgtgga tcgacgctgg aactcagata ttcttttcct acgcaatctg cctgggggcc
     3241 atgacctcac tgggaagcta taacaagtac aagtataact cgtacaggga ctgtatgctg
     3301 ctgggatgcc tgaacagtgg taccagtttt gtgtctggct tcgcaatttt ttccatcctg
     3361 ggcttcatgg cacaagagca aggggtggac attgctgatg tggctgagtc aggtcctggc
     3421 ttggccttca ttgcctaccc aaaagctgta accatgatgc cgctgcccac cttttggtct
     3481 attctgtttt tcattatgct cctcttgctt ggactggaca gccagtttgt tgaagtcgaa
     3541 ggacagatca catccttggt tgatctttac ccgtccttcc taaggaaggg ttatcgtcgg
     3601 gaaatcttca tagccatctt gtgtagcatc agctacctgc tggggctgac gatggtgacg
     3661 gagggtggca tgtatgtgtt tcaactcttt gactactatg cagctagtgg tgtatgcctt
     3721 ttgtgggttg cattctttga atgttttgtt attgcctgga tatatggcgg tgataactta
     3781 tatgacggta ttgaggacat gattggctat cggcctgggc cctggatgaa gtacagctgg
     3841 gctgtcatca ctccagctct ttgtgttgga tgtttcgtct tctcgcttgt caagtatgta
     3901 cccctgacct acaacaaagt gtaccggtac ccggattggg caattgggct gggctggggc
     3961 ctggcccttt cctccatgct gtgtatcccc ttggtcattg tcatcctcct ctgccggacg
     4021 gagggaccgc tccgcgtgag aatcaaatac ctgataaccc ccagggagcc caaccgctgg
     4081 gctgtggagc gtgaaggggc cacacccttt cactcccgag taaccctcat gaacggcgca
     4141 ctcatgaaac ccagtcacgt cattgtggag accatgatgg gatccgacta caaagaccat
     4201 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     4261 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     4321 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     4381 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     4441 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     4501 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     4561 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     4621 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     4681 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     4741 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     4801 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     4861 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     4921 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     4981 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     5041 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     5101 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     5161 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     5221 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     5281 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     5341 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     5401 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     5461 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     5521 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     5581 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     5641 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5701 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5761 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5821 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5881 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5941 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6001 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6061 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6121 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6181 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     6241 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6301 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6361 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6421 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6481 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6541 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6601 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6661 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6721 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6781 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     6841 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6901 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     6961 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7021 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7081 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7141 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7321 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7441 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     7501 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     7561 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7621 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7681 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7741 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7801 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7861 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7921 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7981 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8041 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8101 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8161 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8221 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8281 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8341 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8401 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8461 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8521 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8581 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8641 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8701 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8761 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8821 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8881 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8941 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9001 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9061 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9121 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9181 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9241 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9301 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9361 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9421 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9481 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9541 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9601 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9661 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9721 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     9781 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     9841 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     9901 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9961 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10021 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10081 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10141 cagtgccaag ctg
//
