LOCUS       V035805                11581 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035805
VERSION     V035805
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2291..3940
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
                     /label="luciferase"
     CDS             3977..5641
                     /label="Slc1a5(NM_009201)"
                     /note="Slc1a5(NM_009201)"
                     /gene="Slc1a5"
     CDS             5648..5674
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5678..5704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5708..5734
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5779..5990
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6003..6271
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6306..7013
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             7023..7076
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7077..7676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7677..8264
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8338..8571
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8643..8764
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8783..8918
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8956..8972)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8980..8996
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9004..9034)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9049..9070
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9358..9946)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10117..10977)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10978..11082)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11556..11572
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct
     2341 tgaggatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
     2401 tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt
     2461 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
     2521 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
     2581 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
     2641 tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt
     2701 gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga
     2761 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa
     2821 tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa
     2881 ctcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt
     2941 cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat
     3001 tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat
     3061 atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct
     3121 tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa
     3181 aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc
     3241 acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg
     3301 acaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga
     3361 taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga
     3421 taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat
     3481 tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg
     3541 gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg
     3601 cttgaagtct ttaattaaat acaaaggata ccaggtggcc cccgctgaat tggagtcgat
     3661 attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc
     3721 cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga
     3781 gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt
     3841 gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga
     3901 gatcctcata aaggccaaga agggcggaaa gtccaaattg gaattcggtg gaggcggatc
     3961 aggaggcggt ggatctatgg cagtggatcc ccctaaggct gaccccaaag gggtagtagc
     4021 ggtggattcc accgcgaacg gtggccccgc gctgggatcc agagaggacc agagtgcgaa
     4081 agcaggtggt tgctgcggtt cccgtgaccg ggtgcgccgc tgcattcgcg ccaacctgct
     4141 ggtgctgctc acggtggctg cggtggtggc tggcgtgggg ctggggctgg gggtctcggc
     4201 ggcgggcggt gctgacgcgc tgggtcccgc gcgcttgacc gctttcgcct tcccgggaga
     4261 gctgctgctg cgtctgctga agatgatcat cctgccgctc gtggtgtgca gcctgatcgg
     4321 aggtgcagcc agcttggacc ctagcgcgct cggtcgtgtg ggcgcctggg cgctgctctt
     4381 tttcctggtc accacactgc tcgcgtcggc gctcggcgtg ggtttggccc tggcgctgaa
     4441 gccgggcgcc gccgttaccg ccatcacctc catcaacgac tctgttgtag acccctgtgc
     4501 ccgcagtgca ccaaccaaag aggtgctgga ttcctttcta gatctcgtga ggaatatttt
     4561 cccctccaat ctggtgtctg ctgccttccg ctcttttgct acctcatatg aacccaaaga
     4621 caactcatgt aaaataccgc aatcctgtat ccagcgggag atcaattcaa ccatggtcca
     4681 gcttctctgt gaggtggagg gaatgaacat cctgggcctg gtggtcttcg ctatcgtctt
     4741 tggtgtggct ctgcggaagc tggggcccga gggtgagctg ctcattcgtt tcttcaactc
     4801 cttcaatgat gccaccatgg tcctggtctc ctggattatg tggtacgcac ccgttggaat
     4861 cctgttcctg gtggccagca agattgtgga gatgaaagac gtccgccagc tcttcatcag
     4921 cctcggcaaa tacattctgt gctgcctgct gggccacgcc atccacgggc tcctggttct
     4981 gcctctcatc tacttcctct tcacccgcaa aaatccctat cgattcctgt ggggcatcat
     5041 gacacccctg gccactgctt tcgggacctc ttctagctct gccaccttgc ctctgatgat
     5101 gaagtgtgta gaggagaaga atggtgtggc caaacacatc agccggttca tcctacccat
     5161 cggcgccacg gtcaacatgg acggggcggc gctgttccag tgtgtggcgg cagtgttcat
     5221 cgcacaacta aacggggtgt ccctggactt cgtgaagatc atcaccatcc tggtcacagc
     5281 cacagcatcc agtgtcggtg cggcagggat ccctgcaggg ggcgtcctca ccctcgccat
     5341 catcctggaa gcagtcagcc tgcctgtgaa ggacatctcc ttgatcttgg ccgtggactg
     5401 gctagtggac aggtcctgta ctgtcctcaa cgtggaaggt gatgcttttg gggctggact
     5461 gcttcagagt tacgtggatc gaaccaagat gccgagctca gagcccgaat tgatccaggt
     5521 gaagaacgag gtgtctctga atccactgcc cctcgccaca gaggagggga atcccctcct
     5581 gaaacagtac cagggaccca ccggggactc cagtgccacg ttcgaaaagg aatctgtcat
     5641 gggatcctac ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta
     5701 tgctagctat ccttatgatg tcccagacta cgcataggcg gccgcgaagg atctgcgatc
     5761 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     5821 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     5881 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     5941 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     6001 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     6061 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     6121 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     6181 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     6241 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg
     6301 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     6361 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     6421 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     6481 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     6541 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     6601 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     6661 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     6721 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     6781 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     6841 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     6901 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     6961 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggatctc
     7021 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga
     7081 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     7141 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     7201 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     7261 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     7321 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     7381 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     7441 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     7501 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     7561 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     7621 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc
     7681 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7741 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7801 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7861 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7921 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7981 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8041 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     8101 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8161 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc
     8221 ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac
     8281 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8341 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     8401 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8461 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8521 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8581 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8641 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8701 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8761 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     8821 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8881 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     8941 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     9001 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     9061 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     9121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9781 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11101 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    11161 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    11221 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    11281 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    11341 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    11401 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    11461 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    11521 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    11581 g
//
