LOCUS V045373 10924 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045373 VERSION V045373 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4950 /label="Sf3b2(NM_030109)" /note="Sf3b2(NM_030109)" /gene="Sf3b2" CDS 4957..5022 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5066..5277 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5290..5558 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5587..5596 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5617..6279 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6349..6402 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6403..7002 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7003..7591 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7665..7898 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7970..8091 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8131..8266 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8299..8315) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8323..8339 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8347..8377) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8392..8413 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8701..9289) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9460..10320) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10321..10425) /gene="bla" /label="AmpR promoter" primer_bind 10899..10915 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcggagca tcccgaacct 2341 cccaaaggag agttgcagtt gccaccgcct ccacctccag gccactatgg agcctgggct 2401 gcgcaggagc ttcaggccag attggcagag ataggagcgc cgatccaggg gagtcgcgag 2461 gagttagtag agcggctgca gacctacacc cgtcagacgg gcattgtcct gaatcgacca 2521 gttttgagag gagaagatgg ggacaaagcg gctcctcctc ctatgtcagc acagctgtct 2581 gggattccta tgccaccacc acctatggga cttccccctc tacagcctcc tccaccacct 2641 ccaccacctc caccaggcct tggccttggc tttcctatgg ctcacccacc aaatctgggg 2701 cccccaccac ctcttcgagt gggtgagcct gtggcgctgt cagaagaaga acggctgaag 2761 ctggcacagc agcaggcagc tttgctgatg cagcaagagg agcgtgccaa gcaggcagct 2821 gtgttaatgg agcaggaacg gcagcaggag atcgccaaga tgggcactgc agtccctcgg 2881 ccgcctcagg atatggggca gctgggtgtc cgtactcctc tggggcctcg cgtagctgct 2941 ccagtaggcc ctgtggttcc cacgcctacg gttttgccca tgggggcacc tgttcctcgc 3001 cctcggggtc cccccccacc ccctggagat gagaacagag agatggatga tccttctgtg 3061 ggccccaaga tcccccaggc tttggaaaag attcttcagc tgaaggagag ccggcaggaa 3121 gagatgaact ctcagcagga ggaggaggaa atggagacag atactcgctc atctctggga 3181 cagtcagcat cagagactga ggaggacacg gtgtccatat ccaaaaaaga gaaaaaccga 3241 aagcgtcgga accgaaagaa gaagaaaaag ccacagcggg tgcgggcagc ctcatcagag 3301 agctctgggg accgagagaa ggactccggt cggtcccgcg gctcggaccc ccctgctgct 3361 gacgtggaga ttgagtatgt gaccgaggag cctgagatct acgagcccaa tttcatcttc 3421 ttcaagagga tttttgaggc tttcaagcta actgatgatg tgaagaagga aaaagaaaag 3481 gagccagaga agcttgacaa gatggagagc tctgcagtcc ccaagaagaa gggctttgag 3541 gaggagcaca aggacagtga cgatgacagc agtgatgatg agcaggaaaa gaagccggaa 3601 gcccctaaac tgtcgaagaa gaagttgcgc cgaatgaatc gttttactgt ggcagaactc 3661 aagcagctgg tggcccggcc tgatgttgtg gagatgcatg acgtgacggc acaggacccc 3721 aagctcttgg tccacctcaa ggccacccgg aactccgtcc ctgtgccacg ccactggtgt 3781 tttaagcgca agtaccttca aggcaaacgg ggcattgaga agcccccctt tgagctgcca 3841 gacttcatca aacgcacagg cattcaggaa atgagagagg ccctgcagga gaaggaagaa 3901 caaaaaacca tgaagtcaaa aatgagagag aaggttcggc cgaagatggg gaagatcgac 3961 attgactacc agaagctaca cgatgccttc ttcaagtggc agaccaagcc gaaactcacc 4021 atccatgggg acctttatta tgaggggaag gagtttgaga cacggctgaa ggagaagaag 4081 cctggagatt tatctgatga gctaaggatt tccttaggaa tgccagtagg acctaatgcc 4141 cacaaagtcc ctcctccgtg gctgattgcc atgcagcgat acgggccacc cccatcctac 4201 ccaaacctga aaatccctgg actgaattca cctatccctg agagctgctc ctttggatac 4261 catgctggtg gctggggcaa acccccagtg gatgagactg ggaagccgct ctatggggac 4321 gtgtttggaa ccaatgctgc tgagtttcag accaaaacgg aagaagaaga aattgaccgg 4381 accccgtggg gggaactgga accatctgat gaagaatcct cagaagagga ggaggaggag 4441 gaaagcgatg aagacaagcc agatgagact ggcttcatca cacctgcaga cagcggcctc 4501 atcactcccg gaggattctc atccgtgcca gctgggatgg agacccctga actcattgag 4561 ctgaggaaga agaagatcga agaggcgatg gatggaagtg agacacctca gttattcact 4621 gtattgccag agaagagaac agccactgtt gggggagcca tgatgggatc cacccacatt 4681 tatgacatgt ccacggttat gagccggaag ggcccagccc ctgaattgca aggtgtggaa 4741 gtagcgctgg cgcctgagga attggagttg gaccccatgg ccatgacgca gaagtacgag 4801 gagcacgttc gggagcagca ggcacaagta gagaaggaag acttcagcga catggtggct 4861 gagcatgctg ctaagcagaa gcaaaagaaa cggaaagctc agccccagga cagccgtggg 4921 ggcagcaaga aatacaagga gttcaaattt ggatccgact acaaagacca tgacggtgat 4981 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc 5041 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 5101 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 5161 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 5221 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 5281 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 5341 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 5401 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 5461 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 5521 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 5581 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 5641 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 5701 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 5761 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 5821 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 5881 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 5941 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 6001 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 6061 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 6121 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 6181 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 6241 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 6301 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 6361 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 6421 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 6481 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 6541 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 6601 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 6661 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 6721 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 6781 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 6841 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6901 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6961 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 7021 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 7081 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 7141 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 7201 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 7261 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 7321 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 7381 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg 7441 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 7501 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 7561 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 7621 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7681 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7741 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7801 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7861 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7921 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 8101 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 8161 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 8221 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 8281 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 8341 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 8401 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 8461 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 8521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 8581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 8641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 8701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 8761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 8821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 8881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 8941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 9001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 9061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 9121 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 9181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 9241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 9301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 9361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 9421 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 9481 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 9541 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 9601 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 9661 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 9721 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 9781 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 9841 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 9901 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 9961 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 10021 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 10081 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 10141 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 10201 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 10261 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 10321 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 10381 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 10441 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 10501 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 10561 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 10621 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 10681 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 10741 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 10801 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 10861 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 10921 gctg //